We narrowed to 569 results for: CIT

1. Making CRISPR Plasmids Using Fragmid

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   Blog Post

   ...which you can also deposit with Addgene!) We’re excited to help the Doench lab share this new tool and ...

2. Progress Towards a PAM-Free CRISPR

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   ...and base editing applications, with high editing capacity in both contexts for NRN PAMs and to a lesser ...

3. CRISPR in the Clinic

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   ...Clinical trials are underway to explore CRISPR’s capacity for both classes of edits in cancer therapy. Blood...

4. High Complexity Golden Gate Assembly with NEB’s DAD

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   ...can find for those parameters. For even more specificity, you can exclude specific sequences or set a ...

5. Viral Vectors 101: Virus Safety

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   ... or 1% iodine are useful. Some organizations or cities may require a higher percentage of the decontaminate...

6. How to Prepare for an Industry Interview

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   ...ve worked hard to earn this opportunity and are excited for this next step. But wait, now you feel a little...

7. Adapting Toehold Switches to Detect Zika Virus

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   ... virus. Our original proof-of-concept work was exciting because it enabled gene circuit-based sensors ...

8. 5 Tips for Troubleshooting Viral Transductions

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   ... efficiency in cells that are sensitive to the toxicity of polybrene (e.g., hematopoietic or primary cells...

9. Plasmids 101: FLEx Vectors

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   ...FLEx switch takes advantage of the orientation specificity of the site-specific recombinases (SSRs) Cre ...

10. Experimenting in an Open Source lab: from CRISPR to Cats

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    ...computer model. When I first heard about it, I got excited from a computer scientist perspective. But, the...

11. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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    ...accelerator program who want us to succeed. We’re excited to see what the next few months have in store for...

12. Tips for a 1st time AAV user (by a Rookie AAV user)

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    ... a few different concentrations of virus, or multiplicity of infections (MOIs), to determine the optimal...

13. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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    ...using different Type IIS restriction enzyme specificities at each step. Factors such as enzyme efficiency...

14. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    ...Drosophila can be used to push your research in new and exciting directions. Drosophila are very easy to manipulate...

15. Truncated gRNAs for Regulating Gene Expression

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    ...mismatch tolerance and, consequently, increased specificity relative to the more commonly employed 20nt gRNAs...

16. Hot Plasmids: Winter 2025

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    ... and subthreshold events, including miniature excitatory postsynaptic potentials (mEPSPs) (Hao and Lee...

17. Magnetic Control of Proteins: More than a Dream

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    ...might help formulate some hypotheses. They are excited to see other researchers from a range of fields...

18. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    ...example of the emission spectra for two fluorophores excited by the violet laser, BV421 and BV711. These “rainbow...

19. Hot Plasmids and Viral Preps - May 2021

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    ...Flp-dependent DREADDs viral vectors. Both inhibitory and excitatory DREADDS from the Gether lab are now available...

20. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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    ... locus. The two methods had similar rates of specificity for the DNA sequence targeted in the experiment...