We narrowed to 702 results for: abo.1

... to just that particular assay.      Fig. 1: A native protein (left) is denatured, allowing the...naturally.  Sample Denaturing  First, let’s think about induced conformation changes. Several types of assays...immunoimaging assays. It can also elucidate information about what a given protein is doing or changing in different...

...do researchers track fluorescently labeled cells? 1. Epifluorescence microscopy This technique uses a ...of the immune system and its dynamics. References 1. Peters, Nathan C., et al. "In vivo imaging reveals...fluorescence microscope where the light source is mounted above (epi) the specimen and the excitation light passes...technique that allows imaging of living tissue up to about one millimeter in depth. Two low-energy photons ...response, but the study of these dynamics was still laborious as epifluorescence microscopy wasn’t able to give...skin. Two-photon intravital microscopy. The video above depicts the infiltration of neutrophils expressing...

...defunct (but fun) plug-in called Maps.  Figure 1: See a starfish? This is actually a map of Joanne ...know better. Networking is not about how many contacts you have; it’s about building genuine relationships...doing with nothing else to talk about. I know absolutely nothing about them and they don’t know me. It...relationships with people you care about knowing. How do you follow up with new connections and build the relationship...your new relationships Here are some ways to go about strengthening a new relationship with authenticity... on LinkedIn and always include a personal note about where you met and what you discussed. Do this in...such as FiercePharma), or sign up to be alerted about their new publications via Pubmed, so you can congratulate...

...help you decide if a master’s might be for you. 1. Are you interested in master’s-level jobs? Ultimately... Resources Resources on the Addgene blog Learn about career planning resources What Do I Do Now? Academic...

...occur, but good performers move around easily  Table 1: Taking it a Step Further - Big Company v. Small Company...students Collaborations may be less “complicated” - for example, easy to initiate a collaboration without...up the pace Constant worry about grants and renewals What is different about non-academia? Scientific...make a decision about something like this without sufficient data? Starting to learn about the diverse options...with scientists just like you What is different about academia? Publishing in peer reviewed journals ...without having to worry about proprietary information Work hours are long, but there is more flexibility with...paths to pursue. Spend some time really thinking about your motivations and what parts of being a scientist...

...sequencing the unique DNA barcodes. Figure 1: Schematic of barcoding with a pooled library. Created...variations in the spike protein, teaching us more about viral evolution and antibody binding. Figure...

...approximately 20 nm of a specific DNA sequence in only 1 min. Using these lentiviral plasmids, catalytically... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here. Listen...

.... You can email her at [email protected].   References 1. Huang, Ally, et al. "BioBits™ Explorer: A modular...student at MIT. While I had always enjoyed learning about biology in high school, it wasn’t until I started...college that I really fell in love with it. Something about being able to actually hold all those seemingly ...seemingly abstract biological reactions that I learned about from textbooks just made everything click in my head...students build circuits in physics class to learn about electronics or mix chemicals together in chemistry...a power source, but now you don’t have to worry about operating the rest of the car as well. Similarly...with us! We’ll work with you to get the word out about your plasmids and their potential educational uses...

...process is explained more fully in the animated Fig. 1 below. Normalization is a fundamental and often understated...”) of DNA, have changed our collective thinking about the feasible size and scope of projects in many ...next generation (NGS) sequencing have prevented laboratories from using these methods for routine processes...daunting for large numbers of samples. As mentioned above, one of the primary technical benefits of plexWell...1ng – 100ng) are normalized to two-fold variation above and below mean read count per sample, and the amount...NGS readout also reveals additional information about the presence of non-plasmid DNA or heterogeneous...   Check out the SeqWell website to learn more about our plexWell kits and services! Many thanks to Joe...

...can also rank the plasmids by abundance, scaled to 1, to create a “cumulative wealth distribution” curve... and we thought it would be a good time to talk about the amplification and verification processes we ... DNA. These reactions should allow us to obtain about 100 to 1,000 bacterial colonies per individual plasmid...from the count_spacers.py file from Feng Zhang’s laboratory. There are a number of other analysis scripts...you have any tips, input, or stories of interest about library use, let us know in the comments! Additional...NGS Sequencing to Validate AAV Preps Learn More about Pooled CRISPR Libraries Resources on Addgene.org...

...participants could share their notes together. Figure 1: Can you draw Spongebob from these instructions without... with the three other organizers. We asked them about their experiences as they navigate the new norm ...pre-workshop questionnaire to the participants to learn about what they were looking to learn in the workshop ...having a stable internet connection,” says Abitua about her workshop. “We made sure that we had a backup...on the Addgene blog Read all of our blog posts about conferences Find 25 tips for working from home ...

...trans-activator Brainbow) (Sakaguchi et al., 2018) (Figure 1). Once introduced, fluorescent proteins were best ...Additional resources on the Addgene blog Read about Brainbow Learn about using AAVs for neuronal tracing Visit...page Additional resources on Addgene.org Learn about Addgene's viral service See our fluorescent protein...

... After all I was only going to be interviewed for 1 hour so having a broad enough insight was going to...for Dummies”. The fastest way to learn something about marketing would be to start reading a broad simple...introduction to the financial world. It teaches you about selling products which is an important part of the...Seán on twitter @Macfhearraigh.    Read more posts about Scientific Career Development ...

...large sequences and even entire genes. Figure 1: Mechanism of CAST systems. A Cas effector (Cascade...These days, it hardly seems like we finish writing about one dual CRISPR-transposon system before another...Programmable gene insertion in human cells with a laboratory-evolved CRISPR-associated transposase. Science...

...extension. Now, they split EB1 into two halves (Fig. 1): one containing the microtubule binding domain and... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.   ...emission maxima toward the red end of the spectrum (above 600 nm) and increasing the brightness. Arne Fabritius...

...produce as many as 700 copies per cell. Figure 1: A plasmid map showing the standard features of a ...last updated Nov 10, 2020. Now that we know all about antibiotic resistance genes, let’s consider another...the replicon. For example, the pMB1 ori maintains about 20 copies per cell, while pUC – which differs by...

...Article contributed by Susanna Bachle Figure 1: Initial steps in the CRISPR-pooled screening using... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.  ...non-overlapping cells. Until now, tools for doing this were labor-intensive and required using manipulations such ...

...and cost for AAV genome sequencing.  Figure 1: The viral genome sequencing workflow. Using this...share the procedures and Python scripts with laboratories and viral vector cores routinely producing AAV...on Addgene.org Find AAVs at Addgene Learn more about Addgene's viral service Find protocols for working...

...net negative charge with 4 negatively charged and 1 positively charged amino acids. Myc tags are frequently...Tags have a lot of major benefits – as discussed above – but there are a few instances when they should... hindrances, and destabilization can be brought about by the tag so it’s important to validate tagged ...

...heat-fixed smear with the crystal violet stain for 1 minute. Rinse the slide briefly with tap water. Flood... it can be used in a lab? Here we’ll be talking about the ins and outs of this classification system, ...The classification system is also useful in a laboratory setting. When designing a plasmid to be inserted...