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  2. Retrovirus Guide

    Type
    Guide
    ...of the transfer plasmid sequences into the host genome. Typically it is the sequences between and including...including the LTRs that is integrated into the host genome upon viral transduction. Most transfer plasmids...First, these viruses were derived from different genomes (MoMLV and MSCV for γ-retrovirus; HIV for lentivirus...: U3; Unique 3'; region at the 3' end of viral genomic RNA (but found at both the 5' and 3' ends of the...Contains sequences necessary for activation of viral genomic RNA transcription. R; Repeat region found within...; Unique 5'; region at the 5' end of the viral genomic RNA (but found at both the 5' and 3' ends of the...

  5. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...RNA Targeting Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

  6. AAV Viral Preps

    Type
    Collection
    ...purity assessment, sterility testing, and viral genome sequencing (VGS). In-stock AAV preps are distributed...

  7. Guide to Using Pooled Libraries

    Type
    Guide
    ...libraries! Genome-wide Screening Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout...genes. Some libraries cover the majority of the genome, while others are restricted to certain gene sets.... Read our CRISPR guide for more information on genome-wide screening with CRISPR/Cas9 . Browse our Viral...Knockout Screening Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens...

  8. Tetracycline Inducible Expression

    Type
    Collection
    ...Contains homology arms for integration into AAVS1 Genomic Safe Harbor Locus. Tet-On 3G On Doyon 58245 pGLTR-X-GFP...Fraser S, Eshkind L, Oesch F, Zabel B. Physiol Genomics . 2002 Dec 3;11(3):115-32. PubMed . Tetracycline...

  9. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Publications Enhancer AAV plasmids are plasmids which use genomic regulatory sequences — termed enhancers — to drive...enhancers in mice. Enhancers were selected from the genome based on their differential chromatin accessibility...enhancers were derived from human, mouse, and macaque genomes. Enhancers were evaluated in mouse brains, using...

  10. Molecular Biology Reference

    Type
    Guide
    ... of short RNAs. Genome Engineering Plasmids - Used to target and edit genomes. Genome editing is most ...thousand basepairs from the entire human genome. Digesting the human genome with restriction enzymes would yield...plasmids upon cell division in addition to their own genomic DNA. Because of this additional replication burden...guide RNAs that target specific locations in the genome. For more information on CRISPR check out Addgene...Viral Plasmids - These plasmids are modified viral genomes that are used to efficiently deliver genetic material... to sequence an entire plasmid or an organism’s genome. One Sanger sequencing reaction would give you ...

  11. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...Yale have made improvements to this system by genomically recoding E.coli (Lajoie et al., Science 2013)...(ca. 2015) described in: A flexible codon in genomically recoded Escherichia coli permits programmable...

  12. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Plasmids: Base Edit Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

  15. Science Guides

    Type
    Guide
    ...immune system in bacteria, have been modified for genome engineering. Find essential background information...genes. Some libraries cover the majority of the genome, while others are restricted to certain gene sets...

  16. AAV for Neuronal Tracing

    Type
    Collection
    ...required for the transcription or replication of the genome within infected cells, but is required for transynnaptic...

  18. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...Chen et al., 2023). High viral titer High vector genome (vg) doses are recommended for all systemic capsid... screening. However, AAV-PHP.AX packages viral genomes at a lower efficiency than AAV9 and thus produces...

  19. Antibody Plasmid Collection

    Type
    Collection
    ...of hybridoma-produced antibodies by CRISPR/HDR genomic engineering. Sci Adv. 2019 Aug 28;5(8):eaaw1822...

  20. Adenovirus Guide

    Type
    Guide
    ...) Glossary Adenoviral Components The adenovirus genome is a linear, 36-Kb double-stranded DNA (dsDNA) ...heavily spliced transcripts. At either end of the genome are i nverted t erminal r epeats (ITRs). Genes ...
Showing: 501 - 520 of 533 results