We narrowed to 586 results for: ret

1. DNA Purification Without a Kit

   Type

   Blog Post

   ...but trace contamination could interfere with interpreting the results of transfection assays. Go forth...

2. SciComm: Taking Science to Elementary and Middle Schools

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   ... itself in terms of what they do with their discretionary money. They also usually have a good event-planning...

3. Isolating Genomic Regions of Interest with the CRISPR System

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   ...Fujii Lab generated a transgenic mouse line and retroviral expression system for using this approach in ...

4. An Introduction to Adenovirus

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   ...kilobases; compared to only 4.7 kb for AAV, 9 kb for retroviruses). The tradeoff is that adenoviral vector expression...

5. Course-Based Undergraduate Research Experiences (CUREs)

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   ...Zuckerman, 1977; Ma et al., 2020). Many have interpreted this trend as evidence that the best teachers...

6. AAVs CREATed for Gene Delivery to the CNS and PNS

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   ...Multi-color labeling of neurons is for more than making pretty pictures (see figure 4). It also enables single-cell...

7. Uncovering Ancestral Bias in CRISPR Research

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   ...and consider bias as a confounding factor when interpreting findings, particularly when researching ancestry...

8. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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   ... try out the JF dyes. Acknowledgements I thank Brett Mensh, a wordsmith soldier of fortune, for helpful...

9. Lentiviral Vector Uses and Overview

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   ...researchers developed viral vector systems based on retroviruses like Moloney murine leukemia virus (MMLV). The...

10. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    ...independently of other viral proteins simplifying the interpretation of results. This system can be used to develop...

11. Cpf1: A New Tool for CRISPR Genome Editing

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    ...cleaves relatively far away from the PAM, NHEJ might retain the PAM site. Therefore, if HDR does not initially...

12. Negotiating Work and Life: How to Find the Joy

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    ...running errands. Give help when you have time, and in return you will get help when you need it. On the work...

13. Analyzing CRISPR Editing Results with ICE from Synthego

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    ...or large deletions, the ability to analyze and interpret data about these types of edits is critical to...

14. Transferable Skills Guide: External Collaborations

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    ...your ideas and don’t be afraid to make something concrete happen. You may have more willing collaborators...

15. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    ...two sequences are necessary and sufficient for retention and replication of the vectors in human cells ...

16. Transferable Skills Guide: Career Planning Resources

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    ... than a CV or resume). If you receive a card in return, make sure to follow up with a professional “thank...

17. CRISPR 101: Any Base Transversion Editors

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    ...adenine transversion editor AYBEv3. This tool still retained considerable A → G editing activity at most sites...

18. Twenty Years of Sharing: Expanding Accessibility

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    ...Lentiviral Guide AAV Guide Optogenetics Guide γ-Retrovirus Guide Protocols Agarose Gel Electrophoresis...

19. Twenty Years of Sharing: Addgene's Viral Vector Service

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    ...vectors in a variety of serotypes, including the retrograde AAVrg and the systemic capsid PHP.eB.   ...

20. Hot Plasmids Spring 2024

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    ...contexts in vitro and in vivo, including in human retinal organoids and live mice. Importantly, REVeRT avoids...