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We narrowed to 765 results for: Mos

Showing: 521 - 540 of 765 results

  3. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...recognize the same epitope of the target protein. Most commonly these antibodies are produced from hybridomas...fragments: An overview and comparison of three of the most versatile biosensor biorecognition elements. Biosensors...

  4. Lentiviral Vector Guide

    Type
    Guide
    ...-G (vesicular stomatitis virus G protein) is the most common envelope gene in all systems, due to a wide...eliminating the need for transactivation by Tat. Most third-generation transfer plasmids also contain ...interest into the transfer plasmid can be done with most standard cloning methods, including restriction ...utilize lentiviral vectors. Some lentiviral plasmids (mostly third-generation) can be used in transient transfections...of viral integration have shown that lentiviruses most often integrate into transcriptionally active regions...process called pseudotyping. VSV-G is by far the most common envelope gene, as it has a wide range of ... should be made on a case-by-case basis. As with most experiments, infection risks occur with contact ...

  5. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...plasmids pX330S-2 to pX330S-(last gRNA number). The 5’ most gRNA is cloned into the Cas9-containing destination...Danilo, et al. “In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system.” ...

  6. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...many applications in biotech, and is considered the most studied Gram-positive bacterium, many of its genes...looking for the production of ECT-DNA (extra-chromosomal telomeric DNA), which accumulates when T-loops...

  7. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...-G (vesicular stomatitis virus G protein) is the most common envelope gene, due to a wide range of infectivity...interest into the transfer plasmid can be done with most standard cloning methods, including restriction ...process called pseudotyping. VSV-G is by far the most common envelope gene, as it has a wide range of ...risk of integrating into proto-oncogenes. As with most experiments, infection risks occur with contact ...envelope protein with broad tropism used to pseudotype most gamma-retroviral vectors. Glossary Term Definition...

  8. Guide to Using Pooled Libraries

    Type
    Guide
    ...screens POSITIVE screen: Apply library Apply selection Most cells die or do not pass selection (in the case ...selection) In parallel, apply library Apply selection; most cells will survive Perform NGS on the surviving ...The selective pressure must be strong enough that most of the cells die, removing their plasmids from the.... Check out our blog posts featuring some of our most popular libraries! Genome-wide Screening Using CRISPR...

  9. Promoters

    Type
    Guide
    ...Core Promoter The core promoter region is located most proximal to the start codon and contains the RNA...factors promote the initiation of transcription. The most 3' portion (closest to the gene's start codon) of...eukaryotes and archaea, however, contain this TATA box. Most prokaryotes contain a sequence thought to be functionally...contains transcription factor binding sites, but mostly contains regulatory elements. Eukaryotic Promoters...

  10. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    ..., Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses, lentiviruses...

  13. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  On the tenth day of CRISPR, Addgene...

  14. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...CRISPR guide is a comprehensive resource that covers most things CRISPR - from nickases to base editing to...

  19. Addgene at SfN23!

    Type
    Blog Post
    ...conference blog posts before you go!  Tips to Make the Most of a Scientific Conference Poster Session TipsHow...
Showing: 521 - 540 of 765 results