Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 772 results for: Mos

Showing: 521 - 540 of 772 results

  2. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...recognize the same epitope of the target protein. Most commonly these antibodies are produced from hybridomas...fragments: An overview and comparison of three of the most versatile biosensor biorecognition elements. Biosensors...

  3. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...plasmids pX330S-2 to pX330S-(last gRNA number). The 5’ most gRNA is cloned into the Cas9-containing destination...Danilo, et al. “In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system.” ...

  4. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...many applications in biotech, and is considered the most studied Gram-positive bacterium, many of its genes...looking for the production of ECT-DNA (extra-chromosomal telomeric DNA), which accumulates when T-loops...

  5. Molecular Biology Reference

    Type
    Guide
    ... from chromosomal DNA. Although they can be found in archaea and eukaryotes, they play the most significant...conclusions that genes encode proteins and reside on chromosomes, which are composed of DNA. These findings culminated...order to isolate a particular gene from human chromosomal DNA, it would be necessary to isolate a sequence...coined the term plasmid, in reference to any extrachromosomal heritable determinant. Plasmids are fragments...relationship with the host cell. Like the bacterial chromosomal DNA, plasmid DNA is replicated upon cell division...DNA from one context to another, such as from a chromosome to a plasmid. These tools are essential to the...recombinant DNA Molecular Cloning Plasmids that are used most commonly in the field of recombinant DNA technology...

  6. Antibody Guide

    Type
    Guide
    ...immune system’s response to foreign bodies, are the most commonly used affinity reagents. Antibodies are ...against the antigen, packaged, and sold. This is the most cost-efficient way to produce antibodies, with the... IgA2 IgD Coexpressed with IgM on the surface of most immature B cells; function is unknown Monomer with...anaphylactic shock Monomer with a valency of 2 IgG The most abundant isotype in blood, also found in tissues...Produced during the primary immune response; found mostly in blood Pentamer with a valency of 10 Antigen ... for exact storage requirements. As a guideline, most antibodies can be stored at 4 °C for short-term ...allow detection of the antibody:protein complex. The most commonly used types of signaling molecules are horseradish...

  7. Adenovirus Guide

    Type
    Guide
    ...is the most popular method for creating first-generation rAdV vectors, which is still the most commonly...range of mild diseases in humans and other animals, most commonly respiratory infections, such as the common...applications. However, this has also made them the most widely used viral vector platform for vaccine design...vectors in order to deliver genetic cargo into cells. Most rAdV vectors are based on adenovirus type 5 (Ad5...autonomously. First-generation rAdV vectors remain the most popular choice due to their well-established methods...interest into the transfer plasmid can be done with most standard cloning methods, including restriction ...plasmid pAdEasy-1 (Addgene #16400) is suitable for most purposes. For especially long transgenes, the use...

  8. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...interest into the transfer plasmid can be done with most standard cloning methods, including restriction ...Human embryonic kidney 293T (HEK293T) cells are the most common platform to produce AAV vectors. Additional...genome at a specific site, the AAVS1 site on human chromosome 19. This integration is mediated by proteins ...molecular weight structures that are maintained extrachromosomally. Random integration of rAAV genomes in non-homologous...for the production of capsid proteins. AAV2 is the most commonly used serotype for rAAV vector construction...delivery of CRISPR/Cas9 elements, making it one of the most common methods for in vivo CRISPR-based genome editing...incompetent and not known to cause disease in humans. Most, if not all of Addgene’s transfer plasmids are replication...

  9. Plan Your Experiment

    Type
    Guide
    ...apply to using CRISPR in other organisms. As with most experiments, you first need to have a biological...experiment. In the table below, we highlight the most common categories of genetic manipulations used ...transcriptase (RT)) Can edit within a wider window than most base editors Editing efficiency can be low, but ...where it makes sense to manually design a gRNA, in most cases gRNA design software is better and more efficient... while testing multiple gRNAs is a good idea for most CRISPR experiments, testing multiple pegRNA designs...) are also relevant. Delivery Using Plasmids The most straightforward method to deliver a CRISPR system...efficiently transfect. However, note that this method is most useful for cell lines that you can transfect at ...

  10. Lentiviral Vector Guide

    Type
    Guide
    ...-G (vesicular stomatitis virus G protein) is the most common envelope gene in all systems, due to a wide...eliminating the need for transactivation by Tat. Most third-generation transfer plasmids also contain ...interest into the transfer plasmid can be done with most standard cloning methods, including restriction ...utilize lentiviral vectors. Some lentiviral plasmids (mostly third-generation) can be used in transient transfections...of viral integration have shown that lentiviruses most often integrate into transcriptionally active regions...process called pseudotyping. VSV-G is by far the most common envelope gene, as it has a wide range of ... should be made on a case-by-case basis. As with most experiments, infection risks occur with contact ...

  11. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    ..., Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses, lentiviruses...

  14. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...-G (vesicular stomatitis virus G protein) is the most common envelope gene, due to a wide range of infectivity...interest into the transfer plasmid can be done with most standard cloning methods, including restriction ...process called pseudotyping. VSV-G is by far the most common envelope gene, as it has a wide range of ...risk of integrating into proto-oncogenes. As with most experiments, infection risks occur with contact ...envelope protein with broad tropism used to pseudotype most gamma-retroviral vectors. Glossary Term Definition...

  15. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  On the tenth day of CRISPR, Addgene...

  16. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...CRISPR guide is a comprehensive resource that covers most things CRISPR - from nickases to base editing to...
Showing: 521 - 540 of 772 results