We narrowed to 620 results for: PROC

1. Finding Your Science Policy Path

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   Blog Post

   ...with alumni before and during your application process so you can learn more! Figure 2: The many...

2. Antibodies 101: Validation

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   Blog Post

   ...identified as your target protein, allowing you to proceed with confidence.  Share your results Ultimately...

3. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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   ...to study gene regulation during developmental processes, CRISPR/Cas9 allows us to delete sequences and...

4. When Fidelity Matters: A frank discussion about ligase fidelity

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   ...amplification using cloned thermostable ligase." Proceedings of the National Academy of Sciences 88.1 (1991...

5. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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   ...Red-mediated recombinants in mismatch repair mutants. Proceedings of the National Academy of Sciences of the United...

6. Typing CRISPR Systems

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   ...alternatives to Cas9. Cas12 is unique because it can process multiple gRNAs under a single promoter, allowing...

7. Antibodies 101: Introduction to Antibodies

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   Blog Post

   ...purposes, antibodies can be used to affect cellular processes in vivo, and these are referred to as functional...

8. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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   Blog Post

   ... Encouraged by the high efficiency and easy procurement of cr:tracrRNA reagents, we scaled up these tagging...

9. Which Fluorescence Microscopy Techniques is Best for Me?

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   Blog Post

   ...with deconvolution, a mathematical software post-processing step that reassigns out of focus light to its...

10. Custom CRISPR Screens & the Green Listed Software

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    Blog Post

    ...genes, Green Listed can help make the gRNA design process quick and easy. Green Listed - a CRISPR Screen ...

11. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    Blog Post

    ...UV-inducible green-to-red fluorescence conversion." Proceedings of the National Academy of Sciences of the United...

12. Special Delivery: Fluorophore Targeting for FRET Studies

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    Blog Post

    ...Ca2+ measurements using improved cameleons." Proceedings of the National Academy of Sciences 96.5 (1999...

13. A Primer on Optogenetics: Introduction and Opsin Delivery

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    Blog Post

    ...surgeries and viral expression, you are ready to proceed to the material science part of optogenetics. In...

14. Optogenetics + CRISPR, Using Light to Control Genome Editing

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    Blog Post

    ...timing, and reversibility of the genome editing process. Read on to learn about the various light-controlled...

15. CRISPR 101: Cytosine and Adenine Base Editors

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    Blog Post

    ...repair. Black arrows indicate natural cellular processes. BER = base excision repair. Note: hypoxanthine...

16. Technical Design of a Western Blot

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    Blog Post

    .... They can be stored at your bench during the procedure and at 4 °C when you’re not running a western....

17. 15 Hot Plasmids from 2017

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    Blog Post

    ... light to alter cell physiology and molecular processes via genetically encoded proteins. PhoCl is a new...

18. Troubleshooting and Optimizing a Western Blot

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    Blog Post

    ...high background or low signal during the imaging process, try adjusting your exposure time first, since ...

19. Viral Vectors 101: Systemic Capsids

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    Blog Post

    ...organs. AAV.CAP-B22 was developed through the same process as AAV.CAP-B10 and shows high transduction levels...

20. Chemogenetics Guide

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    Guide

    ...receptors potently activated by an inert ligand. Proc Natl Acad Sci USA. 104:5163–68. PMID: 17360345 (...and biological light sources for opsin activation. Proc Natl Acad Sci USA. 113:E358-67. PMID: 26733686 (...with a specifically designed Gi-coupled receptor. Proc Natl Acad Sci USA. 95:352–57. PMID: 9419379 (Link...G protein regulation of β cell function in vivo. Proc Natl Acad Sci USA. 106:19197–202. PMID: 19858481...to be involved in a wide variety of biological processes, including initiating signaling pathways in inflammation...