We narrowed to 583 results for: RET

1. Fluorescent Proteins 101: When GFP lets you down

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   Blog Post

   ...PMCID: PMC3129320.  18. Mastop, Marieke, et al. "A FRET-based biosensor for measuring Gα13 activation in...

2. Replacing Paper: Tips for Choosing an Electronic lab Notebook

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   Blog Post

   ...information is consolidated to one place, easily retrievable, searchable and traceable. However, switching...

3. Antibodies 101: Introduction to Immunofluorescence

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   ...Example steps include: permeabilization, antigen retrieval, and sectioning. Blocking Block is used to...

4. Antibodies 101: Single Chain Fragment Variables (scFvs)

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   ...drawback for therapeutic applications where longer retention times often increase therapeutic efficacy (Ahmed...

5. Antibodies 101: Normalization and Loading Controls for Western Blots

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   ...promise it isn’t.) The intuitive, qualitative interpretation of this gel is misleading. We can see why on...

6. Antibodies 101: Beyond Surface Labeling

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   ...cytosolic proteins, as well as proteins of the secretory pathway. Those can include cytokines and chemokines...

7. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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   Blog Post

   ...infections not usually seen in healthy individuals. Antiretroviral therapy (ART) can virtually eliminate plasma...

8. Thoughts on the Future of Research 2015 Boston Symposium

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   ...academic research. Panelist Moon Duchin provided a concrete example of how underrepresented individuals are...

9. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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   ...and -2. The general structure of Brainbow-1.0 is retained in Brainbow-3.0, but with mOrange2, EGFP and mKate2...

10. How to Design Your gRNA for CRISPR Genome Editing

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    ... effective, so long as care is taken in the interpretation of results by requiring multiple sequences ...

11. CRISPR 101: Epigenetics and Editing the Epigenome

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    ...with BioRender.com. DNA Methyltransferase MQ1 Margaret Goodell's lab has deposited pcDNA3.1-dCas9-MQ1...

12. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

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    ...notebooks also play a role here by making it easier to retain searchable, thorough notes on how lab procedures...

13. Viral Vectors 101: Optogenetic Tools

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    ...proteins responsive to light in combination with retinal) variations designed to give researchers flexibility...

14. Antibodies 101: Multiplex Immunofluorescence

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    ... patterns with several overlapping targets to interpret. Check out (Jambor et al., 2021) for more guidelines...

15. Plasmids 101: Repressible Promoters

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    ...9485-4. Epub 2018 Nov 3. PMID: 30392122. Pfeiffer, Barret D., et al. “Refinement of Tools for Targeted Gene...

16. Is this the right place for me? 8 tactics for choosing a lab

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    ...friend didn’t heed this warning and profoundly regretted it when the same thing happened to her a year ...

17. Technique: Probe Phage Genomes for Host Binding Proteins

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    Blog Post

    ...-too-common) event that homology-based searches return no candidates. Our strategy: Express random fragments...

18. Generating Mouse Models Using CRISPR/Cas9

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    ....     References Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini ...

19. Building Global Connections with the International Mentorship Program USA-EUROPE

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    Blog Post

    ...Conference highlights included remarks by the Spanish Secretary of Research, Development, and Innovation, D. Carmen...

20. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    Blog Post

    ...reactions that complicate the effectiveness and interpretation of RNAi. Fortunately, researchers studying ...