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... the samples have been added to the qPCR plate. Start by adding water, then SYBR master mix, then the ... initial value. If the Ct value of the standard starts to drift, it’s time to make a new one. When developing...

...UFC903024 50 mL LoBind tubes, VWR 76289-498 Before Starting Wipe down all pipettes and equipment with 10% ...if this has not already been done. We typically start with about 250 mL of supernatant and add 250 µL ...

...generated at any time. Considerations Before You Start Some cell lines tend not to grow well in sparse/...may be optimal for different cell types. Before starting this procedure, be sure that the cell pool has...

...membrane. Store at 4 °C. Considerations Before You Start The health of the HEK293T cells is critical for ...confluencies. This AAV production protocol should be started with cells that are ~80% confluent (center panel...

...with effective gene silencing: Starting at 25nt downstream of the start codon (ATG), search for 21nt sequences...

...properties: Length of 18-24 bases 40-60% G/C content Start and end with 1-2 G/C pairs Melting temperature (...

...information page. The next day you will be able to start an overnight culture for plasmid DNA prep the following...

... out more protocols and videos to help you get started in the lab! Introduction There are four biosafety...

...popular technique is to draw in discontinuous lines. Start by streaking a vertical line of bacteria along one...

...purify DNA fragments based on size. The procedure starts with standard agarose gel electrophoresis, which...

...tube. Place tube in a thermocycler programmed to start at 95°C for 2 minutes. Then, gradually cool to 25...

... out more protocols and videos to help you get started in the lab! Introduction For many experiments, ...

... out more protocols and videos to help you get started in the lab! Introduction A water bath is a piece...

...fused to your gene of interest, in frame with the start codon or tag sequences (where appropriate). The ...

...Inoculating a Liquid Bacterial Culture Protocol Getting Started with Tissue Culture Tips and tricks for working...

... out more protocols and videos to help you get started in the lab! Introduction A centrifuge is a common...

...Introductory techniques designed to help you get started in the lab. Name Description (Link opens in a new...

... to 56 °C for 30 min. Considerations Before You Start The health of the target cell line is critical for...

...intend to do a miniprep, you will usually want to start 2 mL in a Falcon tube. For larger preps, you might...

...Get started with Gibson Assembly Cloning!...