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We narrowed to 568 results for: VIT

Showing: 561 - 568 of 568 results
  1. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...transduction efficiency in vitro than any wild-type serotype, and a very high infectivity across a broad range...are transfected into cells which then act as in vitro factories for the new viral particles in the laboratory...with rAAV vectors include the Allen Mouse Brain Connectivity Atlas and the Janelia Campus MouseLight project... RNA translation, and promotes RNA transcript longevity. Located between WPRE and 3'ITR. Not required ... elements into the genome, often altering gene activity. Latent infection A state where a pathogen is ...Optogenetics Biological technique to control the activity of cells, often neurons, with light. Pseudotyping...proteins from different species. Used to alter infectivity and tropism. Recombinant adeno-associated viral...
  2. Lentiviral Vector Guide

    Type
    Guide
    ...packaging gene env (usually VSV-G due to wide infectivity) VSV-G (vesicular stomatitis virus G protein)... gene in all systems, due to a wide range of infectivity for different cell types (known as tropism). ...common envelope gene, as it has a wide range of infectivity across many cell types and enhances the stability...also gained popularity to further enhance both infectivity and specificity. Pseudotyped lentiviral vectors...2007). Role of PSIP1/LEDGF/p75 in Lentiviral Infectivity and Integration Targeting. PLoS ONE , 2 (12),...proteins from different species. Used to alter infectivity and tropism. Replication-competent The ability...
  3. Molecular Cloning Techniques

    Type
    Guide
    ...exonuclease activity of T4 DNA polymerase. In LIC, the T4 DNA polymerase’s exonuclease activity creates “...free nucleotide. T4 will resume its polymerase activity, add back the free base, and become stuck at this...principle to Gibson cloning, but instead of an in vitro reaction with purified enzymes, it takes advantage...
  4. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...packaging gene env ; usually VSV-G due to wide infectivity VSV-G (vesicular stomatitis virus G protein) ...common envelope gene, due to a wide range of infectivity for different cell types (known as tropism). ...common envelope gene, as it has a wide range of infectivity across many cell types and enhances the stability...proteins from different species. Used to alter infectivity and tropism. Replication-competent The ability...
  5. Antibody Guide

    Type
    Guide
    ...(~25 kD), but tend to have lower affinity and longevity. Single-domain antibodies - Single-domain antibodies...monoclonal antibodies tend to have less cross-reactivity and give lower background signals than polyclonal...interest is knocked down or out, and antibody reactivity is compared between the wild-type and the knockdown...cell line with low or no expression; antibody reactivity is then compared between the knock-in cell line...
  6. Adenovirus Guide

    Type
    Guide
    ... elements into the genome, often altering gene activity. Oncolytic Referring to the destruction of cancer...proteins from different species. Used to alter infectivity and tropism. pShuttle Class of shuttle/transfer...
  7. Science Guides

    Type
    Guide
    ...molecules, which allows for specific control of activity. Read More CRISPR Class 2 C lustered R egularly...
  8. Promoters

    Type
    Guide
    ...transcription of a gene is initiated. Promoters are a vital component of expression vectors because they control...
Showing: 561 - 568 of 568 results