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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 830 results for: eed

Showing: 561 - 580 of 830 results

  1. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...recruitment of katanin to that anchor. So, you’ll need to express both the EB3N-VVDfast-iLID anchor module...many tools available for controlling cell dynamics. Need a refresher on VVDfast, iLID, LOV, CRY2-CIB, and...

  2. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ... avoid overlap with this optogenetic tool, they needed to create a calcium sensor with the following characteristics...could not be used as the excitation wavelengths needed would also activate the optogenetic accuators. ...

  3. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ... antibody specificity and antigen structure. Proceedings of the National Academy of Sciences 76:3116–3120...nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences 76:4350–4354...

  4. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ... expression levels of your gene of interest, as needed. Controlling the expression levels of your gene...approach is that, since there is no selection, you will need to screen somewhere between one hundred and one ...

  6. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...acronyms, so if you're new to viral vectors or simply need a refresher, we've included a glossary at the end...order to produce rAAV vectors, three plasmids are needed: Transfer plasmid (also known as cis plasmid) —.... The cell line used for rAAV vector production needs to express the adenovirus E1 gene, which is crucial...especially if any additional regulatory elements are needed. One strategy to overcome this obstacle, has been...with contact to mucous membranes or broken skin. Needle sticks and ripped gloves are common points of entry...

  7. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...protein of interest in a single cell? Or perhaps, you need to analyze a specific subset of cells in a complex... being used together in an experiment, you would need a way to determine the relative contribution of ...

  8. Typing CRISPR Systems

    Type
    Blog Post
    ...foreign DNA with unique properties. This led to a need to develop a classification system for the array... tools using a Cascade complex is tricky, as you need to ensure every unit of the complex expresses at...

  9. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...reconstituting, choosing your colors depending on the needs of your experiment (Figure 3). And using self-labeling...TMP-tag or tetracysteine labeling dyes.  If reaction speed is a priority, SNAP-Tag or HaloTag have been shown...

  11. Chemogenetics Guide

    Type
    Guide
    ...targets for development into chemogenetic tools. Need a refresher on G-protein coupled receptors? Read...back-metabolism of CNO to clozapine, demonstrating the need for alternative DREADD ligands. Compound 21, Deschloroclozapine...while PSAMs have more than one domain, both of which need to be expressed. However, neuronal control through...experiment, an AAV-encoded chemogenetic plasmid may need to be targeted to specific tissues, cell types or...

  12. Molecular Biology Reference

    Type
    Guide
    ...the genetic code and provides all the information needed for cells to make proteins and other molecules ...sequencing. To understand Sanger sequencing, we first need to understand DNA replication. DNA is a double helix...pieces of a 2,000 piece puzzle. A scientist would need to run a ton of Sanger sequencing reactions on different...Sequence FLAG DYKDDDDK HA YPYDVPDYA His HHHHHH Myc EQKLISEEDL V5 GKPIPNPLLGLDST Xpress DLDDDDK or DLYDDDDK ...

  14. Addgene's eBook Collection

    Type
    Guide
    ...Not sure where to start? Need a quick reference? Addgene's eBooks are here to help!... Collection Not sure where to start? Need a quick reference? Addgene's eBooks are here to help! 0 of 0...101 blog series, this eBook covers everything you need to know about plasmids, from parts, nomenclature...

  15. Optogenetics Guide

    Type
    Guide
    ...Channelrhodopsin from Volvox carteri 570 Chronos High-speed, light-sensitive channel from Stigeoclonium helveticum...When designing your optogenetics experiment, you’ll need to pick both an opsin and a delivery system. Here...experimental design will determine whether you’ll need short or long periods of neuronal activation/inactivation...

  16. Guide to Using Pooled Libraries

    Type
    Guide
    ...amplified before you conduct your screen. If you need to amplify the library, please refer to the depositor...adequately represented in the population, you’ll need to infect many more cells than the number of plasmids...
Showing: 561 - 580 of 830 results