We narrowed to 469 results for: Lif

1. Protocol - Bacterial Transformation

   Type

   Protocol

   ...plasmid DNA for the purposes of storage and amplification. Higher efficiency cells are more important ...

2. KLF Research Plasmids

   Type

   Collection

   ...with diverse roles in processes such as cell proliferation, differentiation, inflammation, metabolism, ...

3. Virus Protocol - Generating Stable Cell Lines

   Type

   Protocol

   ...polyclonal populations of resistant cells start proliferating and the individual wells become confluent, expand...

4. AAV Titration by qPCR Using SYBR Green Technology

   Type

   Protocol

   ...point standard curve. Figure 2: Example of the amplification plots obtained from an AAV sample. Each curve...

5. Cancer Research Plasmids and Resources

   Type

   Collection

   ... Lab for use with the Gateway® Cloning Platform (Life Technologies™) to permit construction of RAS pathway...

6. DNA Service - Cloning Grade DNA

   Type

   Collection

   ...reducing the time to experiments by removing the amplification and extraction steps required when one receives...

7. TALEN Engineering

   Type

   Collection

   ... transcriptional activator target sites and to simplify the construction of TALEN expression plasmids ...

8. Botman-Teusink Yeast FP Collection

   Type

   Collection

   ...together with the protothropic cassette can be amplified with the following primers: FW 5'–GGTGACGGTGCTGGTTTA...

9. Malate Dehydrogenase CUREs Community Collection

   Type

   Collection

   ... STEM Attitudes in CUREs. Callahan KP et al. CBE Life Sci Educ. 2022 Dec;21(4):ar74. PMID: 36206327 (Link...

10. Synthetic Biology - Overview

    Type

    Collection

    ...Jeff Tabor Jan-Willem Veening Christopher Voigt Clifford Wang Ron Weiss Synthetic biology describes the...

11. AAV ddPCR Titration

    Type

    Protocol

    ...pipette tips and gently touch the bottom of the well. Lift the tips ~1 mm. Touch the side of the well and tilt...

12. Antibody Production

    Type

    Collection

    ...transfection. This residual plasmid is extracted and amplified with primers targeting the heavy chain and/or ...

13. Antibody Plasmid Collection

    Type

    Collection

    ...conformational trapping of membrane proteins. Elife. 2018 May 24;7. and Generation of synthetic nanobodies...

14. Worm Expression Resources

    Type

    Collection

    ...used as a genetic model organism due to their rapid life cycle (3 days from egg to adult) and easy lab maintenance...

15. Allen Institute for Cell Science Plasmid Collection

    Type

    Collection

    ...CTCF-binding sites 170818 PCNA-mEGFP AICSDP-116 mEGFP Proliferating cell nuclear antigen (PCNA) DNA replication ...

16. Plant Plasmids and Resources

    Type

    Collection

    ...system based on tobacco rattle virus (TRV) that simplifies gene silencing and genome editing logistics -...

17. Kazuhiro Oka Lentiviral Vectors

    Type

    Collection

    ...pAdx-CMV-LacZ (Addgene #73345) is uniquely suited to simplify the cloning process as it can be used in blue–...

18. TALEN Guide

    Type

    Collection

    ...with ZF arrays, if not slightly lower. Figure 2: Simplified representation of the Voytas/Bogdanove Golden...

19. Lentivirus ddPCR Titration

    Type

    Protocol

    ...pipette tips and gently touch the bottom of the well. Lift the tips ~1mm. Touch the side of the well and tilt...

20. Viral Production

    Type

    Collection

    ...genomic DNA. This DNA is then used as a template to amplify a portion of the intended lentiviral insert. During...