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  1. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...the mutant versions of SpCas9 and SaCas9, which recognize PAM sequences other than NGG (Table-1). Type...or destruction of any restriction enzyme (RE) recognition site, RE analysis of PCR product could be used...

  2. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...target DNA (Cpf1 requires only a crRNA for target recognition and cleavage). C2c2 (now referred to as Cas13a... or C base pair. In bacteria, once Cas13a has recognized and cleaved its target RNA sequence as specified...

  3. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...optional fluorescence of FPs with facile antibody recognition of epitope tags. Thus they have created antigenic...imaging set-up. In contrast, smFP tags can be recognized by distinct, fluorophore-labeled antibodies with...

  4. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...ori typically contains iterons that serve as recognition sites for replication initiation proteins. These...depends on whether the host has the components to recognize the ori and allow for replication. However, if...

  8. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...considerations are to make sure your secondary 1) will recognize the species of your primary antibody and 2) is...primary antibody, first, remember that antibodies recognize very specific 3-dimensional epitopes. Different...

  9. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...regions of any G-protein-coupled receptor (GPCR) recognizing a specific ligand (Ino et al. 2022). The oxytocin...more than 20 other MTRIA sensors based on GPCRs recognizing various neurotransmitters, neuropeptides and ...

  11. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...that is methylated at the adenosine of the GATC recognition site. Transformation: After the PCR reaction,...can be achieved using a restriction enzyme that recognizes a unique site in the coding region (preferably...

  13. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...plasmid between the T-DNA borders (yellow) are recognized by the Vir genes on the helper plasmid and used...with chemical cargoes in plants enabled by a biorecognition motif. Nat Commun 11: . https://doi.org/10.1038...

  15. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...ability of Type IIS enzymes to cut outside their recognition site, and permits DNA fragments with compatible...assembled. Scientists can engineer unique enzyme recognition sites that flank a DNA module in an inverse orientation...

  17. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...directors, we can't celebrate Addgene without recognizing our incredible depositors and requestors. Thank...

  18. Call for Guest Bloggers

    Type
    Blog Post
    ...techniques that simplify and expedite research. We recognize, however, that we can’t do it all ourselves. Therefore...

  21. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...protein from Neisseria meningitidis, Nme2Cas9, that recognizes a simple dinucleotide PAM (N4CC), lending to ...

  25. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ... The UBMTA used by Addgene is internationally recognized as being protective of both the providing institution...

  31. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...short epitope GCN4. GCN4 recruits GFP fused to the cognate scFV antibody, which is expressed from a separate...

  33. Hot Plasmids - October 2022

    Type
    Blog Post
    ...incorporated into your protein of interest and recognized by reliable antibodies. It is derived from the...

  37. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...treating the protein with enterokinase, which recognizes the 5 amino acids on the C terminal of the tag...

  42. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...virus-isolated topoisomerase I as this enzyme recognizes the DNA sequence 5´-(C/T)CCTT-3' and digests ...

  49. The MTA at Addgene

    Type
    Blog Post
    ... the recipient organization upon purchase. We recognize exceptionally fast tech transfer offices with ...
Showing: 101 - 150 of 258 results