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We narrowed to 400 results for: pho;

Showing: 101 - 150 of 400 results

  1. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...inactivate the fluorophores in the sample, either by chemical treatment or photobleaching or by stripping...and that each secondary has a different color fluorophore that you can image separately on your microscope...labeling sequentially with one, inactivating the fluorophores, then labeling with the other, you can still...using primary antibodies that are conjugated to fluorophores. However, the primary-secondary workflow (a....fluorescently-conjugated streptavidin to get more fluorophores per antibody, or conjugate with an enzymatic...

  2. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...last year, Verkhusha added two far-red light photoactivatable (PA) near-infrared fluorescent proteins (FPs...fluorescent proteins, iRFP670 and iRFP720, as photoacoustic contrast agents for two-color imaging in animals...genetically-encoded probes by making them brighter, more photostable, smaller, and less toxic to cells. They’d like...751-4. doi:10.1038/nmeth.2521) Far-red light photoactivatable near-infrared fluorescent proteins engineered...: 10.1038/ncomms3153. PubMed) Multicontrast photoacoustic in vivo imaging using near-infrared fluorescent...

  3. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...for up to 60% of the cell culture contamination (Uphoff, 2002). Mycoplasmas are considered to be one of...from lowered pH due to microbial contamination. Photo by Kaustubh Kishor Jadhav. Mycoplasmas can remain...for at least two or three days before sampling (Uphoff and Drexler, 2011). Culturing on agar plates/broth...works in the tissue culture hood while wearing PPE. Photo by Kate Harten DeMaio. Cell lines and reagents... of mycoplasma species. J Microbiol .44: 42–49  Uphoff Cc, Drexler Hg (2002) Comparative Antibiotic Eradication...10.1290/1071-2690(2002)038<0086:caeomi>2.0.co;2 Uphoff CC, Drexler HG (2011) Detecting Mycoplasma Contamination...

  4. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...SL-insensitive fluorophore (LSSmOrange) as an internal control and a SL-sensitive fluorophore (circularly...three-dimensional cultures that mimic aspects of organ morphology and physiology in vitro and have emerged as a...investigating genetic mechanisms related to morphogenesis, cell fate specification, and human disease....relies on the fluorescence ratio between the 2 fluorophores, rather than on the fluorescence intensity, ...

  5. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...signal-to-noise ratio Less chance of phototoxicity Reduced photobleaching Simplified, long-term single-molecule...enough signal. This workaround risks cellular phototoxicity and eliminates the possibility of long-term ...a higher signal-to-noise ratio with a lower photobleaching rate. Given the power of SunTag, they attempted...Protein Oligomerization Learn about Small Molecule Fluorphores Resources on Addgene.org Find Fluorescent Protein...

  6. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    ...contributed by Leah Schwiesow Calcineurin is a protein phosphatase that responds to rising intracellular calcium... of Ca2+ are high in the cell, calcineurin dephosphorylates the protein CZI, which in turn binds to calcineurin-dependent... intercalating fluorescent dyes which can cause photo-induced DNA breakage and structural deformation,...reversible and do not cause structural deformation or photo-induced damage. These fusions can also function ...

  7. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    ...migration of a cancer cell line equipped with photodissociable EB1 could be stopped in areas with blue light...manipulating and studying cellular dynamics. The photoinducible control of protein function is more specific...signal. Prior to the development of these probes, fluorophore binding aptamers and MS2-binding fluorescent ...contain a smaller RNA tag, are less prone to photobleaching, and achieve better signal in vivo as compared...aptamers or MS2 tags using the same number of fluorophores. You can find plasmids containing the cobalamin...

  8. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ...fluorescent protein joins mTFP1 and mWasabi, as fluorophores from Allele Biotechnology that now can be deposited...brightest teal fluorescent proteins that were photostable and had a high 480/530 nm emission ratio. They...mWasabi is 1.6-fold brighter than EGFP and is photostable. It also has narrow excitation and emission peaks...useful in multicolor labeling particularly with fluorophores that excite in the violet range. For example...Directed evolution of a monomeric, bright and photostable version of Clavularia cyan fluorescent protein...

  9. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...sfCherry2 sfCherry2(1-10) and sfCherry2(11), also photo-activatable variant PAsfCherry2(1-10) Improved split...iRFP713. Requires presence of heme oxygenase for chromophore formation (abundant in eukaryotes) A Near-Infrared...domain. Requires presence of heme oxygenase for chromophore formation (abundant in eukaryotes) Bright monomeric...typically irreversible and requires some time for chromophore maturation, splitFAST displays rapid and reversible...flexibility in color labeling with exogenous fluorophores, which can even be bright enough for single-...

  10. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ... molecules, which are much brighter and more photostable than fluorescent proteins, making them ideal ...versions. ASAP5 can be imaged using one- or two-photon microscopy to detect action potentials and subthreshold...fluctuations in awake behaving mice through two-photon microscopy at depths up to 600 µm. Figure...recognizes the disease-associated version of Tau (phospho-Tau). We hope these will become valuable tools ...

  11. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...example, if your flow cytometer reads out the fluorophores FITC and PE on the same laser, you will need...deciding to use them, using a tool like BioLegend’s Fluorophore Guide Chart.  Pro tip! Some antibodies go by ...change the compensation values for whichever fluorophore is present in the sample (APC in the case of ...other channels you’re using first. A different fluorophore channel may be affecting your populations and... this population is double-positive for both fluorophores and that there is no issue with compensation...

  12. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...NgAgo DNA guide is inexpensive and can be 5’-phosphorylated in house using a simple PNK reaction or can... the instability of the supposedly alien 5’-phosphorylated ssDNA guides, which might be rapidly degraded...things (101 lessons on Spanish, C++, guitar, photography) and often dreams of a sunny day in Edinburgh...

  13. DNA Purification Without a Kit

    Type
    Blog Post
    ...molecules and coaxes it into sticking to the hydrophobic glass filter. Each glass filter can capture up... only requires incubating the columns with 1M phosphoric acid for 3 minutes followed by a short centrifugation...purification columns could also be cleaned with 1M phosphoric acid and re-used, but trace contamination could...

  14. DNA Service - Cloning Grade DNA

    Type
    Collection
    ... and 260/280 ratio has been determined by spectrophotometer. Other methods of quantification may yield...

  15. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ... Figure 1: Plasmid mix to label neuronal morphology (eYFP) and the synaptic protein PSD95 (PSD95-...control   Figure 2: Expression of a morphological marker (eYFP) and synaptic marker (PSD95-mcherry...experiment it is possible to compare neuronal morphology and synaptic connectivity before and after the...Micron Plasmid Attaches Covalently to DNA via a Phosphotyrosyl Linkage.” Molecular and Cellular Biology 5(...

  17. A Control for All Seasons

    Type
    Blog Post
    ...well-studied kinase known to prevent tau hyperphosphorylation. You could run brain samples from both groups... molecular weight shifts indicative of tau phosphorylation. By including the positive control, you will...controls are single stained samples for each fluorophore and are critical to analyze multiplex flow cytometry...

  18. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ... the phosphatase you choose. CIP (calf alkaline phosphatase) or SAP (shrimp alkaline phosphatase) are ...overhangs after digestion, you will need to use a phosphatase to prevent re-circularization of the backbone... treat your digested backbone plasmid with a phosphatase prior to the ligation step or prior to the gel...

  19. Editor's Choice, July 2016

    Type
    Blog Post
    ...CRISPR variant going forward.   Special Delivery Fluorophore Targeting for FRET Studies While I love all of...This post describes how to target and use FRET flurophores for a variety of applications. While editing ... to study biological phenomena and about how fluorophores other than fluorescent proteins can be incredibly...Comparison to Cas9 and NgAgo Special Delivery: Fluorophore Targeting for FRET Studies Gendered Innovations...

  20. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...et al. "A rhodopsin is the functional photoreceptor for phototaxis in the unicellular eukaryote Chlamydomonas...Deisseroth, combined optical and genetic methods to photo-stimulate mammalian neurons and alter synaptic events...rhodopsins, light-sensitive proteins that absorb photons for energy conversion or the induction of intra...resulting viral-infected neurons are subsequently photo-stimulated through fiberoptic cannulas directly ...

  21. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...started!   Thanks for all your hard work!   Fluorophores When is a Monomer not a Monomer? The Top Three...Better Dyeing Through Chemistry & Small Molecule Fluorophores In this post, Luke Lavis from Janelia Research...fluorescent proteins are incredibly useful, chemical fluorophores can still be used in a wide variety of experiments...experiments. Read more to find chemical fluorophores for your next experiment. Nucleic acid based machines...

  22. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...protein could cleave DNA in vitro, directed by phosphorylated DNA guides. Unfortunately, Swarts et al. were...in GC-rich regions. DNA guides NgAgo uses 5’ phosphorylated DNA guides (so called “gDNAs”) rather than ...

  24. Distribution to Industry

    Type
    Collection
    ...through our website. We cannot accept orders by phone, fax, or email. Pricing Addgene is a nonprofit organization...

  25. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...assume that anyone reading this article has a smartphone nearby. We tailor these devices to our own interests...have relied more and more heavily on adapting my smartphone to help me with my research in terms of preparedness...instead conveniently provide them on your smart phone. 1. Lab Archives by LabArchives The single, most...with colleagues and link your notebook from your smartphone to your computer. Lab Archives will help both...

  26. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...questions. Don't try to get all panelists on the phone at once – you won't get as much out of it and it...better at remembering these things on a relaxed phone call than when first confronted on a stage in front...front so the interactions are better. Have a good microphone system ready for each panelist if the room needs...what happens to the attention of the audience – phones come out, postures shift, they are checking out...

  27. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ... wavelengths of low photosynthetic activity. This still allows photosynthetically active wavelengths to... coral’s symbiotic dinoflagellates that are photosynthetic (Salih et al., 2000). Chromoproteins also enhance... (2000) Fluorescent pigments in corals are photoprotective. Nature 408:850–853 . https://doi.org/10.1038...

  28. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...and ecology can make for beautifully compelling photos and videos, while molecular, micro, and cell biology...Easy to host guests remotely. Easy access from smartphones with low data requirements.  No visuals. Variety...academic to informal writing can difficult.  Photography Medium (high for microscopy) Visually compelling...context via still images. Some things cannot be photographed or need text to properly contextualize. Graphics...

  29. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ... oligonucleotides called morpholinos. When injected into zygotes, morpholinos complementary to a target...activity and does not work easily in zebrafish.) Morpholinos are a convenient and fast way to deactivate genes...whether a gene is deactivated via mutation or morpholino. This observation unlocked novel research into...

  30. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...fiber photometry.  Figure 1: Characterization of iGluSnFR4 in mice. A) Scheme for two-photon (2P)...iGluSnFR4s produces larger signal-to-noise ratios in two-photon imaging, making it ideal for video recordings of...dTomato, displaying characteristic PV basket cell morphology. Adapted from Furlanis, Dai, Leyva Garcia et ...

  32. Open Enzyme Collection

    Type
    Collection
    ... DNTT 165555 pOpen-ALPI CIP (calf intestinal phosphatase) Return to top Additional Resources Bacterial...

  34. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ...fabulous photos again this year! Why #DecktheLab? Help us spread some cheer by snapping a photo of you... Festivus— you name it, we want to see it! All photos tagged with #DeckTheLab throughout the month of...

  35. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...effector cells of the immune system - cytotoxic T lymphocytes (CTLs), natural killer (NK) cells, and helper...consistently shown promise in reducing leukemia and lymphoma and are in their clinical trial phase. In all ... this results in frequent physiological and morphological changes. Tumors differ in their genotypic and...expressing CD19 chimeric antigen receptors for acute lymphoblastic leukaemia in children and young adults: a phase...

  36. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ... primary antibodies that are conjugated to a fluorophore, to avoid the use of secondary antibodies in .... Because the signal comes from the antibody-fluorophore conjugate, and is not expressed by the cell itself...localization. These could be visualized using fluorophore-conjugated antibodies (i.e., immunofluorescence...unconjugated primary antibody, then visualized using a fluorophore-conjugated secondary antibody or Fab. Image from...

  37. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...Halloween festivities for years – searching our photo archives I found pictures back through 2009, though...contests were indeed occurring earlier but that the photo evidence was destroyed to protect the innocent revelers...Facebook and checkout our FB page on Friday for more photo evidence.        ...

  38. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...often used to monitor enzymatic activity or phosphorylation over time. You can also use biosensors to follow... Rosella Autophagy Sensor Read about Aptamer Fluorophores Use CRISPRainbow for Genome Visualization Resources...

  39. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...unmodified LC3, GFP-LC3 is conjugated to phosphatidylethanolamine (PE) and recruited to autophagasomal membranes...Takeshi, and Daniel J. Klionsky. "The quantitative Pho8Δ60 assay of nonspecific autophagy." Methods in enzymology...protein oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy Resources...

  40. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...inside cells that are on the move, such as T lymphocytes. Griesbeck and his colleagues began by isolating...and high resolution functional tracking of T lymphocytes, too, making them a versatile tool for application... functional in vivo imaging of neurons and T lymphocytes. Nat Methods. 2014 Jan 5. doi: 10.1038/nmeth.2773...

  42. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...range of characteristics (color, brightness, photostability, maturation, oligomerization), many of which...incorporation of biosensors, protein evolution lineages, 2-photon spectra, more elaborate spectrum analysis tools...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...

  45. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...grow over time, but being interested in the same phosphate of your favorite kinase is a good start. Check...next to someone and introducing yourself. Put your phone away! This is a natural time to meet someone since...meals, sit with people you don’t know and put your phone away (did I say that already). Start with, “Is this...

  46. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    .... Use the "Contact info" link right below their photo. 2) Personalize all connection requests LinkedIn...invitation. Don’t try to connect from the LinkedIn phone app either as this also results in a generic invitation...for the picture. It tends to look like a police photo if you look straight at the camera. Pro tip: Turn...

  47. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...conjugated to a detection substrate, such as a fluorophore, or a conjugated secondary antibody that will...of primary antibodies conjugated to different fluorophores or a panel of primaries that was raised in different...isotype or species, then you can choose different fluorophore-conjugated secondary antibodies specific to each...

  49. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGCTGATACTCCTGCTGGtggcgtcccgtctggaTGCTGTGAGCTTTCCTGA Semaphorin 6bb TAL3356 & TAL3357 TTGAAAAGTGCTGAGAAAtgtgatgtcattggagTTTGATTTAGGAGACAAA...
Showing: 101 - 150 of 400 results