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We narrowed to 319 results for: virus

Showing: 101 - 150 of 319 results

  1. Viral Production

    Type
    Collection
    ... specific virus in order to generate high quality preparations. After production, all virus preps are ...Resources Lentivirus Production Titer Quality Control Resources Overview of Viral Production Viruses are generated...experiment using our virus protocols . Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral...preps available from Addgene. After receiving your virus from Addgene, read our detailed recipient instructions...contamination. In the event of contamination, all of the virus produced in the line will be taken off-line and ...preps available from Addgene After receiving your virus from Addgene, read our detailed recipient instructions...instructions for next steps. Plan an experiment using our virus protocols . Browse our plasmid collection of popular...

  2. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...how a phage recognizes its host Bacteriophages (viruses that prey on bacteria) may be the most numerous...Simpson, Sacher, and Szymanski in January 2016 in Viruses (5). Please refer to the paper for more details...Function of Modified Bases in Bacteria and Their Viruses." Chemical Reviews (2016). PubMed PMID: 27319741...Receptor Binding Proteins from Bacteriophages." Viruses 8.1 (2016): 17. PubMed PMID: 26761028. PubMed Central...

  3. Hot Plasmids and Viral Preps - May 2021

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    Blog Post
    ...severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus may acquire mutations to escape ...transporting proteins in yeast by using murine polyomavirus virus-like particles (MPyV VLPs ). This artificial...structures. We have expanded our recombinase-dependent virus selection to now include Flp-dependent DREADDs viral...

  4. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    Blog Post
    ... the most studied viruses. Adenoviruses are non-enveloped double-strand DNA viruses with a genome of around... DT (2012) Adenovirus Strategies for Tissue-Specific Targeting. In: Applications of viruses for cancer...DG, Sontheimer EJ, Weng Z, Gao G, Xue W (2015) Adenovirus-Mediated Somatic Genome Editing of Pten by CRISPR...

  5. Save Time with Transient Plant Leaf Transformations

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    Blog Post
    ...silencing genes Virus induced gene silencing (VIGS) is a transient technique that uses a virus and the plant's...: PMC6585625. Unver, Turgay, and Hikmet Budak. "Virus-induced gene silencing, a post transcriptional gene...

  6. Negative Can Be Positive: Open AAV Data with Addgene

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    Blog Post
    ...promoter to use, or if and how much the spread of the virus to adjacent areas impacted the experiments we wanted...and compatibility of recombinant adeno-associated virus under conditions commonly encountered in human gene...2019). Lot-to-Lot Variation in Adeno-Associated Virus Serotype 9 (AAV9) Preparations. Human Gene Therapy...

  7. Plasmids 101: Multicistronic Vectors

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    Blog Post
    ...tricks discovered in positive single-stranded RNA viruses to allow for the efficient translation of multiple...IRES sequences have been discovered - many from viruses, but also some from cellular mRNAs. What they all...5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in...single mRNA. IRES was originally discovered in poliovirus RNA, where it promotes translation of the viral...vectors. These peptides, first discovered in picornaviruses, are short (about 20 amino acids) and produce...eukaryotic mRNA directed by a sequence derived from poliovirus RNA. Pelletier et al (Nature. 1988 Jul 28;334...efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice. Kim...

  8. Beginner's Guide to Viral Vectors

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    Blog Post
    ...quick reference when you're designing your next virus experiment. Subscribe to Viral Vector Blog Posts...important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses in this easy-to-use guide...? Check out our viral vector guide pages, our Lentivirus FAQ, and our viral service pages. Click on the...

  9. What the HEK?

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    Blog Post
    ...will highlight some of those applications here.   Virus production HEKs are the go-to ‘packaging cells’ ...with the SV40 origin of replication. To generate a virus, you need only transfect the ‘helper’ plasmids (...Netherlands. Transformation of HEK cells with adenovirus 5 DNA was postdoc Frank Graham’s 293rd experiment...transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells. FASEB J. (2002...

  10. Celebrate Open Data Day with Addgene’s AAV Data Hub!

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    Blog Post
    ...recognition for sharing your data. Materials: What virus and serotype did you use and what animal subject... region and cell class ended up expressing your virus? If you were using a genetic tool, did it operate...

  11. Serotype Testing AAV

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    Collection
    ...that was used to make the virus. For more details, see our FAQ on What is virus associated DNA, and why ...Cart *Each viral service request will also include virus associated DNA (vdna), which is a sample of the ...vector packaging service, but lets us know what viruses would be helpful to the scientific community....

  12. Brain Initiative Collection

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    Collection
    ...100798-AAV1 pAAV-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing; to be coinjected with ... 100799-AAV1 pAAV-TREtight-mTagBFP2-B19G helper virus for monosynaptic tracing; to be coinjected with ...millisecond resolution optogenetics. Cre-dependent virus Edward Boyden 108422-AAV5 pAAV-CAG-FLEX-Archon1-...pAAV-hSyn-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under synapsin promoter...pAAV-CAG-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter ...pAAV-hSyn-dLight1.2 To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoter...

  13. AAV ddPCR Titration

    Type
    Protocol
    ... C = Copies/µL D = Dilution factor of virus V = Volume of virus in reaction mix (4 µL) Sample Data When...Titration by qPCR AAV Viral Service Viral Vector Guides Virus Blog Posts Molecular Biology Reference Introduction...Water Procedure Preparation Before handling any viruses, get materials ready. Ensure that primers, probe...contamination of the PCR reagents and the NTC with virus. Pre-warm the 96-well plate sealer by gently touching...plate and pipette 5–10 times to mix. Dilute the virus as follows: Mix dilutions thoroughly, but pipette...

  14. AAV Titers: Where Do They Come From And What Do They Mean?

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    Blog Post
    ...reserve lots, but if you’re reordering the same virus feel free to email us and we can do our best to ...Characterization of a recombinant adeno-associated virus type 2 Reference Standard Material." Human gene ...Addgene.org Find All Available AAV Learn More about Virus Production Find Viral Vector Protocols ...

  15. Hot Plasmids: Fall 2024

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    Blog Post
    ...epitope derived from the P and V proteins of Simian virus 5 (SV5) (Randall et al., 1987). The epitope (GKPIPNPLLGLDST...matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked...characterization of monoclonal antibodies to simian virus 5 and their use in revealing antigenic differences...

  16. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post
    ...service as one of those stories. Viral vectors are viruses engineered to be safely used to deliver a genetic...especially those familiar with adeno-associated viruses (AAVs), will know just how tricky these tools can... Kamens, J., & Fan, M. (2019). Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation...

  17. Hot Plasmids Spring 2024

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    Blog Post
    ...the SARS-CoV-2 nucelocapsid protein, one of the virus’s most abundant proteins and a critical component...microglia was further enhanced by packaging the virus as self-complementary AAV (scAAV). However, scAAV...Targeting of Resident Microglia with Adeno-Associated Virus. bioRxiv 2023.12.12.571321; doi: https://doi.org...

  18. Lentivirus Plasmids

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    Collection
    ...Plasmids Additional Resources: Lentivirus Guide Addgene's Viral Service Virus Biosafety Blog: Tips for Titering...and useful types of viruses used in research. Lentivirus can transduce both dividing and non-dividing ...considerations to evaluate when working with lentivirus: these viruses are based on HIV-1, which may require ...cells without a significant immune response. These viruses also integrate stably into the host genome, enabling...biosafety procedures. Additionally, because this virus integrates randomly into the host genome, it has...Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses, are some of the most common and...Viral production Frequently asked questions about lentivirus use Glossary of commonly used lentiviral terms...

  19. Retrovirus Plasmids

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    Collection
    ...Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an RNA virus comprised of its genome and ...cell, the virus uses the reverse transcriptase in tow to generate a DNA provirus. This provirus then gets...discuss retrovirus, they typically are referring to a subset of retroviruses called γ-retroviruses. γ-Retroviruses...Frequently asked questions (FAQ) about retrovirus Popular γ-Retrovirus Envelope and Packaging Plasmids Common...γ-Retroviral Plasmids Additional Resources: γ-Retrovirus Guide Biosafety Guide Lentiviral Plasmids AAV...transgene expression. Some disadvantages of γ-retroviruses are that they can only transduce dividing cells...breakdown of the nuclear envelope). Additionally, γ-retroviruses integrate randomly into the host's genome, which...

  20. Plasmids 101: TOPO Cloning

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    Blog Post
    .... The TOPO technique specifically uses Vaccinia virus-isolated topoisomerase I as this enzyme recognizes.... Shuman S. "Recombination mediated by vaccinia virus DNA topoisomerase I in Escherichia coli is sequence...

  21. AAV Viral Preps

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    Collection
    ...Ready-to-use adeno-associated virus (AAV) available from Addgene's viral vectory packaging service. Browse...Service AAV Browse In-Stock AAV Adeno-associated virus (AAV) vectors are safe and useful gene delivery ...Cell Ablation Monosynaptic Neuronal Tracing Rabies Virus-Based Serotype Serotype Testing Samples Small samples...

  22. COVID-19 Resources

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    Collection
    ...Background The Coronavirus Disease-2019 (COVID-19) pandemic is caused by a novel virus strain from the... elucidate as much as they can about this novel virus. It is now known that SARS-CoV-2 entry, like that...suitable for generating SARS-CoV-2 spike pseudotyped virus. It also lists several luciferase and fluorescent... Spike protein constructs for making VSV pseudo virus Mammalian constructs Due to the size and complexity...mediating the entry of SARS-CoV and SARS-CoV-2 viruses. ( 1 ) TMPRSS2 - a serine protease that primes ...primes the SARS-CoV-2 S protein and is involved in virus entry into cells. ( 2 ) FURIN - an enzyme that cleaves... to the SARS-CoV-2 S protein and is involved in virus entry into cells. ( 4 ) Cathepsin L - a cysteine...

  23. Viral Prep Service for Plasmids and Pooled Libraries

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    Collection
    ...catalog items. Virus-associated DNA Each viral vector service request will also include virus-associated ... the original plasmid that was used to make the virus. In order for Addgene to provide viral vector services... libraries. Viral preparation options include lentivirus and AAV. Viral...Order a high-quality and ready-to-ship AAV or lentivirus prep for your next experiment today! Browse AAV...

  24. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    Blog Post
    ...full-fledged pandemic. Containing the spread of this virus has become an utmost priority for countries around...method has been optimized to detect the SARS-CoV-2 virus directly using saliva without having to perform ... not give a positive result for highly similar viruses. Unlike Cas12a, Cas13a does not require strict ... methods.   Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR based DETECTR assay...in the saliva (Lucia et al., 2020). Detecting coronavirus using CRISPR-based SHERLOCK (Specific ​H​igh ​...ultrasensitive and visual detection of novel coronavirus SARS-CoV-2 Sensitivity: 4.6-11 copies/microliter...2020). An ultrasensitive, rapid, and portable coronavirus SARS-CoV-2 sequence detection method based on...

  25. A Novice's Perspective on Neuroscience - SfN2017

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    Blog Post
    ...familiar, ready-to-use adeno associated virus (AAV) and lentivirus are now available from Addgene. While...repository, you can instead have Addgene make the virus for you. Neuroscientists are particularly interested...

  27. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ...mammal called alpaca could neutralize the SARS-CoV-2 virus. But while the early results were promising, the...projects to a halt. None of us works directly with viruses or transmissible diseases, so we were left with...origins trace back to March 2020, when the first coronavirus lockdown forced us to put our research projects...

  28. AAV Q&A with Tim Miles

    Type
    Blog Post
    ... transfection efficiency when delivering these viruses via retro-orbital vs. tail vein systemic injections...Additional Resources on the Addgene Blog Using virus in your research - a primer for beginners Adenoviral...user Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page Subscribe to Viral Vectors...

  29. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...now important for accelerating research into this virus. One such example is cell lines. The American Type...Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-...Infected with Severe Acute Respiratory Syndrome Coronavirus. JVI 81:813–821 . https://doi.org/10.1128/jvi...

  30. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...this is likely to shear the envelope of your virus. o. Virus may be stored at 4°C for a few days, but should...decrease the efficiency of the virus, so Addgene recommends that you use the virus immediately or aliquot the...0.05-1 mL virus (add 0.5 mL for a high MOI, and 0.1 mL for a low MOI). Scale the amount of virus added depending....g. BL2+) suitable for handling HIV-derivative viruses. For transient knockdown of protein expression,...protocol can be scaled to produce different amounts of virus as needed. Day 1: a. For each plasmid to be transfected...infection time to between 4 – 20 hours. Remove the virus-containing media and replace with fresh media. Do...DS et. al. Cell . 2003. 115:199-208. PubMed . Lentivirus-delivered stable gene silencing by RNAi in primary...

  31. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    Blog Post
    ...Santana et al., 2020) and plants viruses (barley yellow striate mosaic virus (Gao et al., 2019) or sonchus... the ubiquitously expressing cauliflower mosaic virus 35S promoter (CaMV 35S) or ubiquitin promoters (...sonchus yellow net rhabdovirus (Ma et al., 2020) have already been shown to be efficient ways to deliver CRISPR-Cas9...Z, Wang Y, Wang X (2019) Rescue of a plant cytorhabdovirus as versatile expression platforms for planthopper...

  32. Open Enzyme Collection

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    Collection
    ...165510 pOpen-DNA Topoisomerase 1B Vaccinia Virus Vaccinia Virus DNA Topoisomerase 1B 165515 pOpen-EcoRIM...165546 pOpen-MMLV_RT (mut H) Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated... pOpen-MMLV_RT (lack H) Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH...

  33. Addgene: The First Twenty Years

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    Blog Post
    ...have a resource like Addgene to all get the same virus.” — Grad student visiting Addgene’s booth at the...Addgene pivoted to support researchers combatting the virus. Our COVID-19 collection, comprised of 1302 plasmids...

  34. Plasmids 101: FLEx Vectors

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    Blog Post
    ...for improvement of optogenetic adeno-associated virus (AAV) vectors. Listed below are a few additional...Sabatini BL. 2012. Novel recombinant adeno-associated viruses for Cre activated and inactivated transgene expression...

  35. University of Florida Serotype Testing Panel for the Eye and Brain

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    Collection
    ...Bogner, et al. 2015. Capsid Mutated Adeno-Associated Virus Delivered to the Anterior Chamber Results in Efficient...rAAV2tYF-CB-hRS1, a Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin. Hum Gene Ther Clin...Transduction of Retina by Recombinant Adeno-Associated Virus Vectors. J Virol . Mar 28;90(8):4215-4231. PMID:...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...

  36. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    Blog Post
    ...neural pathways was 8.5 x 10e10 GC/mL. The lot of viruses used (100798-AAV, lot #v15287, titer 1.7x10e13 ... (2018) An Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor. J Virol...Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations. Front Synaptic Neurosci 12. https...

  38. Lentiviral Prep Service

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    Collection
    ... controls Puromycin Doench , Root Cas9 Lentivirus Cas9 viruses can be used to interrogate the genome in...fluorescence. Morris Control Lentivirus Browse our list of available viruses based on plasmids in our repository...guarantee viral service, but lets us know what viruses would be helpful to the scientific community.... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently...Pooled Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that are currently in ... viral preparation from the inventory below. Lentiviruses are distributed as purified viral particles ...

  39. Top Requested Plasmid of 2017 - pMD2.G

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    Blog Post
    ...proteins are a key component of lentiviruses. These proteins coat the virus and help determine the types ... of 2017 is the lentivirus envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope vector... producing our ready-to-use lentivirus preps. You can find our lentivirus production protocol on our website...titering your lentivirus in another blog post. If you need a quick primer on how lentiviruses work, check...widely used and requested because it enables lentiviruses to infect many different types of cells. This...check out our lentivirus guide page. For more protocols and information, be sure to check out the Trono Lab...Additional Resources Quick Guide to All Things Lentivirus 5 Tips for Troubleshooting Viral Transductions...

  41. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ... enable the virus to enter host cells. To do this, they injected the spike protein from two different ...spike proteins in cell culture, and inhibit these viruses.  But llama immunization has its drawbacks: it...bind to coronavirus spike proteins (Wrapp et al., 2020), which sit on the surface of coronaviruses and enable...different coronaviruses into a llama over several days and isolated special camelid antibodies that bind to the...Structural Basis for Potent Neutralization of Betacoronaviruses by Single-Domain Camelid Antibodies. Cell ... carriers collection Find other COVID-19 and Coronavirus Plasmids & Resources ...

  43. CRISPR Cheat Sheet

    Type
    Blog Post
    ... that allow the bacteria to target and destroy viruses that infect them. Commonly referred to as a bacterial...

  44. Video Library

    Type
    Protocol
    ...Protocol AAV Purification Purify adeno-associated virus from a preparation produced in mammalian cells AAV...measure the physical titer of your adeno-associated virus preparation. AAV Titration by qPCR Using SYBR Green...

  45. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...almost three decades, recombinant Adeno-associated virus (AAV) has been widely used to deliver genetic tools..., myelocytomatosis viral oncogene (myc), simian virus 5-derived epitope (V5), the synthetic peptide FLAG...

  46. AAVs in Retinal Gene Therapy

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    Blog Post
    ...-Associated Virus (AAV) While other viral vectors such as adenovirus (AdV) and lentivirus (LV) are known...Resources on the Addgene Blog Adeno-Associated Virus (AAV) for cell and gene therapy AAVs for genome ...prior with the death of Jesse Gelsinger in an adenovirus-based gene therapy clinical trial gone horribly...

  47. Hot Plasmids - August 2020

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    Blog Post
    ...improve the catalytic efficiency of tobacco etch virus protease (TEV), a highly sequence-specific protease... from SARS-CoV-2 sequences and live influenza A virus. It was demonstrated to work in human lung epithelial...

  48. The Challenges of Cell Culture

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    Blog Post
    ...propagate viruses in cell culture; if there were no cells to infect, there would be no viable viruses to study...

  49. Light Sheet Fluorescence Microscopy

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    Blog Post
    ...However, we recently developed AAV (adeno-associated virus)-based fluorescent labeling methods that can be ...animals such as rats (17).  In addition, the use of viruses allows much better spatiotemporal control over ...

  50. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...expression promoter from the simian vacuolating virus 40 Constitutive  May include an enhancer. PGK1...Strong plant promoter from the Cauliflower Mosaic Virus Constitutive  Active in dicots, less active in monocots...mammalian expression promoter from the human cytomegalovirus Constitutive  May contain an enhancer region...expression mRNA  Strong insect promoter from baculovirus Constitutive  Commonly used in expression systems...
Showing: 101 - 150 of 319 results