We narrowed to 132 results for: 112
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TypeBlog Post...CRISPR-Cas9. Science 346:1258096 . https://doi.org/10.1126/science.1258096 Gaudelli NM, Komor AC, Rees HA,... TALENs. Science 333:307–307 . https://doi.org/10.1126/science.1207773 ...
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The Pleiades Promoter Project
TypeCollection...intron-lacZ/NLS Ple56 DDC pEMS1125 EGFP/NLS Ple57 DDC pEMS1126 EGFP/NLS Ple58 DDC pEMS1127 EGFP/NLS Ple58 DDC...pEMS1417 EGFP/NLS Ple112 HCRT pEMS1052 cre/EGFP/NLS Ple112 HCRT pEMS1418 EGFP/NLS Ple112 HCRT pEMS1583 intron-lacZ...NLS Ple49 DBH pEMS1520 intron-lacZ/NLS Ple50 DBH pEMS1112 EGFP/cre/NLS Ple50 DBH pEMS1361 EGFP/NLS Ple51...DDC pEMS1529 intron-lacZ/NLS Ple59 DDC pEMS1128 EGFP/NLS Ple59 DDC pEMS1530 intron-lacZ/NLS Ple60 DRD1 ...EGFP/NLS Ple63 DRD1 pEMS1168 EGFP/NLS Ple64 FEV pEMS1129 EGFP/cre/NLS Ple64 FEV pEMS1398 EGFP/NLS Ple66...EGFP/NLS Ple84 GAL pEMS1180 EGFP/NLS Ple85 GCHFR pEMS1122 EGFP/cre/NLS Ple85 GCHFR pEMS1414 EGFP/NLS Ple87...intron-lacZ/NLS Ple89 GFAP pEMS1376 EGFP/NLS Ple90 GFAP pEMS1121 EGFP/cre/NLS Ple90 GFAP pEMS1377 EGFP/NLS Ple92... -
Brain Initiative Collection
TypeCollection...expression of dLight1.1 under CAG promoter Lin Tian 111261-AAV9 pAAV-hSynapsin1-axon-GCaMP6m For enriched ...enriched expression of GCaMP6m in axons Lin Tian 111262-AAV1 pAAV-hSynapsin1-axon-GCaMP6s For enriched expression...plasma membrane calcium pump hPMCA2w/b Baljit Khakh 112000-AAV1 pAAV-hSynapsin1-axon-GCaMP6f For enriched ...enriched expression of GCaMP6f in axons Lin Tian 112004-AAV9 pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2 For enriched...axons with cytosolic expression of mKate2 Lin Tian 112005-AAV5 pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 For...axons with cytosolic expression of mRuby3 Lin Tian 112005-AAV9 pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 For...axons with cytosolic expression of mRuby3 Lin Tian 112006-AAV9 pAAV-hSynapsin1-GCaMP6s-P2A-mKate2 For co-... -
New England Biolabs Cell-Imaging Plasmid Collection
TypeCollection...localization 101129 pSNAPf-Cox8A Control Plasmid SNAP-tag Control for mitochondrial localization 101124 pSNAPf-H2B...localization 101125 pCLIPf-NK1R Control Plasmid CLIP-tag Control for cell surface localization 101127 pMCP-tag...Plasmid SNAP-tag Control for nuclear localization 101123 pSNAPf-ADRβ2 Control Plasmid SNAP-tag Control for...Empty backbone for transient mammalian expression 101126 pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone...ACP/MCP-tag Control for cell surface localization 101128 pACP-ADRβ2 Control Plasmid ACP/MCP-tag Control ... -
Biosensor AAV Preps
TypeCollection...9 Wilson 111262 pAAV-hSynapsin1-axon-GCaMP6s Syn axon-GCaMP6s none Constitutive 1 Tian 112000 pAAV-hSynapsin1...Sensor: iGABASnFR 112159 pAAV.hSynap.iGABASnFR Syn iGABASnFR none Constitutive 1 Looger 112163 pAAV.hSyn-FLEX.iGABASnFR...Syn GCaMP6m none Constitutive 1, 5, 9 Kim , GENIE 111261 pAAV-hSynapsin1-axon-GCaMP6m Syn axon-GCaMP6m none...axon-GCaMP6f Syn axon-GCaMP6f none Constitutive 1 Tian 112005 pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3 Syn axon-GCaMP6s...axon-GCaMP6s bicistronic mRuby3 Constitutive 5, 9 Tian 112010 pAAV-hSynapsin1-FLEx-axon-GCaMP6s Syn axon-GCaMP6s...pAAV.hSyn-FLEX.iGABASnFR Syn iGABASnFR none Cre dependent 1 Looger 112164 pAAV.hSyn-FLEX.iGABASnFR.F102G Syn iGABASnFR.F102G... -
Adeno-associated virus (AAV) Plasmids
TypeCollection...Program 112863 pAAV2/7 AAV7 AAV packaging plasmid, expressing Rep2 and Cap7 James M. Wilson 112864 pAAV2...Helper Plasmid ID Plasmid Serotype Description PI 112867 pAdDeltaF6 All Helper plasmid for AAV packaging...Service at Addgene ID Plasmid Capsid Description PI 112862 pAAV2/1 AAV1 AAV packaging plasmid, expressing ...plasmid, expressing Rep2 and Cap8 James M. Wilson 112865 pAAV2/9n AAV9 AAV packaging plasmid, expressing...and Cap11 Meghan Rego , Addgene Research Program 112866 pAAV2/rh10 AAVrh10 AAV packaging plasmid, expressing... -
Trimmer Lab NeuroMab Collection
TypeCollection... scFv [N112/16] N112/16 scFv Kir2.1 K+ channel Mouse Mouse 206770 Kir2.1 K+ channel scFv [N112B/29] N112B... channel [N112/16R] Kir2.1 K+ channel Mouse Mouse IgG2a 206613 Anti-Kir2.1 K+ channel [N112B/29R] Kir2.1...K+ channel [N112B/22R] Kir2.1 K+ channel Mouse Mouse IgG2a 220401 Kir2.1 K+ channel [N112B/30R] Kir2.1...Human Mouse 199423 Kir2.1 K+ channel scFv [N112B/14] N112B/14 scFv Kir2.1 K+ channel Rat Mouse 199424 ...Human Mouse IgG2a 177496 Anti-Kir2.1 K+ channel [N112B/14R] Kir2.1 K+ channel Mouse Mouse IgG2a 177497 ...channel [N25/35R] Kir2.3 K+ channel Human Mouse IgG2a 182112 Anti-Kv2.2 K+ channel [N372B/1R] Kv2.2 K+ channel...Human Mouse IgG2b 199408 Anti-Kir2.1 K+ channel [N112B/14R-2b] Kir2.1 K+ channel Rat Mouse IgG2b 199409... -
Celebrating the 2024 Nobel Prize in Chemistry
TypeBlog Post...Science, 302(5649), 1364–1368. https://doi.org/10.1126/science.1089427... -
Rinehart Lab Phosphoprotein Reagents
TypeCollection...iSPI_pSer_Subpool#1 Plasmid 112031 pNAS1b split mCherry 14-3-3β ctrl Plasmid 112030 pNAS1b split mCherry NEDD4...NEDD4 WW2 neg ctrl Plasmid 112029 pNAS1b split mCherry NEDD4 WW2 pos ctrl Plasmid 111883 pNAS1b split ...BL21ΔserB Plasmid 34624 SepOTSα tRNA-Sep ( aka. pCAT112TAG-SepT) Plasmid 34623 SepOTSα SepRS/EF-Sep ( aka...Science , 333 (6046), 1151–1154. https://doi.org/10.1126/science.1207203. PMID 21868676 (Link opens in a... -
Easing the Protein Purification Process with pCri
TypeBlog Post...expression and purification." PloS one 9.11 (2014): e112643. PubMed PMID: 25386923. PubMed Central PMCID: PMC4227841... -
Hot Plasmids - October 2022
TypeBlog Post...Biology, 5(12), 3610–3616. doi: https://doi.org/10.1128/mcb.5.12.3610-3616.1985. ... -
Antibodies 101: Fc Effector Functions
TypeBlog Post...cancer immunotherapy.Sci. Adv.8,eabl9171(2022).DOI:10.1126/sciadv.abl9171 More resources on the Addgene blog... -
CASTing Off for New Shores in Human Genome Editing
TypeBlog Post... Science, 388(6748), eadt5199. https://doi.org/10.1126/science.adt5199 Additional Resources on the Addgene... -
Tetbow: Bright Multicolor Labeling for Neuronal Tracing
TypeBlog Post... or with AAVs (prepared with Addgene #104110 - #104112) (Figure 2). We’ve also created constructs with... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post...phage." Journal of Biological Chemistry (2012): jbc-M112. PubMed PMID: 22915587. PubMed Central PMCID: PMC3476254... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog Post...BioTechniques 43:466–470. https://doi.org/10.2144/000112585 Ding T, Huang C, Liang Z, Ma X, Wang N, Huo ... -
Revamp Your Lab Meetings With Creative Virtual Collaboration
TypeBlog Post...Knowledge. Science 316:1036–1039 . https://doi.org/10.1126/science.1136099 ... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog Post... Coronavirus. JVI 81:813–821 . https://doi.org/10.1128/jvi.02012-06 Ou X, Liu Y, Lei X, Li P, Mi D, Ren... -
New and Upcoming Viral Vectors - May 2020
TypeBlog Post...11447 AAV5 pAAV-Ef1a-fDIO mCherry Karl Deisseroth 112677 AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP... -
Plasmids 101: Screens vs. Selections
TypeBlog Post... 343(6166): 80-84 (2014). doi: https://doi.org/10.1126/science.1246981. Leemhuis, H., Kelly, R.M., Dijkhuizen...