We narrowed to 91 results for: 5-Oct

...transcription. The edited DNA strand displaces the unedited 5’ flap and the resulting heteroduplex is resolved by...CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids...

...phosphoserine directly into recombinant proteins (5). Optimization of this system defined three components...into recombinant proteins, such as the kinase MEK1 (5). MEK1 is inactive without requisite dual phosphorylation...307.5717 (2005): 1969-1972. PubMed PMID: 15790858. 5. Park, Hee-Sung, et al. "Expanding the genetic code...contributed by guest blogger Natalie Niemi, a postdoctoral fellow at the Morgridge Institute for Research...blogger Natalie Niemi! Natalie Niemi, Ph.D., is a postdoctoral fellow at the Morgridge Institute for Research...

...your target gene! Tanenbaum et al., Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. Multi-bit genetic...create a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed.... Boncompain et al., Nat Methods 2012. Mar 11;9(5):493-8. MXS cloning platform for imaging and flow...for further chaining. The MXS-chaining kit contains 5 empty chaining vectors plus a destination vector and...half-life of both the luciferase mRNA (3’UTR AUUUA [5 consecutive]) and protein (C-terminal CL1 & PEST)....different promoters and/or allow for insertion at the 5′ or 3′ end of the sgRNA (i.e. TOP1 or TOP2 constructs...Translational Sequence (SITS). This sequence bypasses the 5′ mRNA cap required for eukaryotic systems and instead...

...circumstance. For a more detailed discussion see [5]. Problems 1 and 2 depend on protein concentrations...PMID: 17586775. PubMed Central PMCID: PMC2206698. 5. Costantini, L.M., et al., Assessing the tendency ...oligomerize under physiologic conditions. Traffic, 2012. 13(5): p. 643-9. PubMed PMID: 22289035. PubMed Central ...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...

...dTAG-13 at doses as high as 20 µM (see Figures 3 and 5 in Nabet et al., Nature Chemical Biology for examples...degradation.. Nature Chemical Biology. May 2018. 14(5): 431-441. PubMed PMID: 29581585. This reference ...contributed by guest blogger Behnam Nabet, a postdoctoral fellow at Dana-Farber Cancer Institute. Targeted...studies (Nabet et al.).   Behnam Nabet, PhD is a postdoctoral fellow at the Dana-Farber Cancer Institute. ...

...Cellular Biology 5(11): 3274–79. PubMed PMID: 2427927. PubMed Central PMCID: PMC369144. 5. Kranz, Andrea...Cellular Level in the Mouse.” Nature Biotechnology 21(5): 562–65. PubMed PMID: 12665802. 9. Villa, Katherine...guest blogger, Katrin Michel! Katrin Michel is a Postdoctoral Researcher at MIT and is fascinated by the question...

...WeShareScience is a free platform for sharing short, 3 to 5 minute videos about research. Videos can be shared... for communicating research. Short videos of 3 to 5 minutes provide more than enough time for researchers...measure the impact of their contribution. It offers a 5-step process and several examples for creating effective...Circadian Rhythm and Melatonin for the Timing of Nocturnal Courtship Vocalization. The video summarizes the...

...consistent 5% positive error across the plate is fine; a 5% positive error on one side and a 5% negative... Probe is on a hairpin structure included in the 5’ primer Yes Yes. Agarose gel  LUX fluorogenic...experimentally.  Probe should have a melting temperature 5-10 degrees above the primer and be at least 25 bp ...Nucleic Acids Research, Volume 28, Issue 19, 1 October 2000, Pages 3752–3761, https://doi.org/10.1093/...

...members to discuss your research plans for the next 5-10 years. You will discuss the focus of your first...for aficionados should be reserved for questions. 5. Understand the job you are applying for It's not ... potential collaborations, critiques of your postdoctoral research, etc. The second question is whether...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...and now serves as the Director of Student and Postdoctoral Programs at the Janelia Research Campus of the...

...methodology with T cell receptor engineering (Ref 5)  as described by Blue Flame Addgene depositor Professor...PMID: 26343581. PubMed Central PMCID: PMC4597191. 5. Eyquem, Justin, et al. "Targeting a CAR to the TRAC...Relationship” by our guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University! Cancer therapeutics...

...job where people have regular schedules. Work 8 to 5 or 7 to 4 or maybe 7 to 6 and 8 to 10 or whatever ...before you get enmeshed in a serious relationship. #5 Thou shalt not sweat the small stuff The house can...And, yes, your partner CAN take the kids to the doctor without your help. Your partner is equally responsible...eight-year-old daughter vehemently reminded her doctor that she was allergic to penicillin – I am much...

...implants have been reported by several groups [4, 5, 6]. These have the obvious advantage of not requiring...PMID: 23580530; PubMed Central PMCID: PMC3769938. 5. Iwai Y, et al. A simple head-mountable LED device...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...

...the desired chromosomal modification can be made in 5 days. Counter-selection with sacB has revolutionized... editing in Escherichia coli." Scientific reports 5 (2015): 15096. PubMed PMID: 28060411. Thomason, Lynn...This post was contributed by Jacob Lazarus, a postdoctoral researcher at Harvard. There’s an astounding...is now an attending physician. He performs post-doctoral research in the lab of Matthew Waldor at Harvard...

... facilitate the isolation of modified cells; and (5) is compatible with the introduction of unique molecular... Find FITS plasmids here! Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard...

... Therapy Vol. 25 No 5. PubMed PMID: 28391961. PubMed Central PMCID: PMC5417792. 5. Naldini,L., 2015. Gene...amaurosis. This is the first of what I, scientists, doctors and patients hope are many more clinical successes...

...surgeries for viral injections can be found here [5, 6]. Opsin transgenic mice Transgenic mouse lines ...10.1038/nmeth.1808. PMID: 22179551; PMCID: PMC4165888. 5. Harris JA, et al. Adeno-associated viral vectors ...activation and silencing. Nature neuroscience. 2012;15(5):793-802. doi: 10.1038/nn.3078.  PMID: 22446880; PMCID...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...

...acquisition and retrieval of contextual fear memories [5].  In a modified behavioral paradigm, the authors ...primate cortex." Frontiers in systems neuroscience 5 (2011). PubMed PMID: 21811444. PubMed Central PMCID...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...

...Make a stock solution of 2.5 mM biotin (50X) because 5 mM is barely soluble at RT. Track expression and purification... GE Life Sciences) or bio-layer interferometry (Octet, Pall ForteBio). Tips for using pMal-T-Avi-His/BirA...for plasmid version 2.0? Instruments such as an Octet may require less in vivo-biotinylated protein (total...

...and to find other posts that deserve a second look. 5 Great Apps for Lab Life This very useful post was ...deposited plasmids on this trip. Here’s to hoping that October is even more awesome than September! Tyler J. Ford...

...consumption in comparison to 37% of the general public (5, 6). Similarly 90% of active research scientists believe...activity while much less of the general public agrees (5, 6). Many of the new administration’s actions, including...active research scientists (scientists who hold a doctorate degree, are currently working full-time, and have...