...differentiate only into myogenic precursor cells [5]. Classification of stem cells Stem cells are classified... are obtained from the inner cell mass (ICM) of a 5-6 day old human blastocyst [6]. They are isolated ...Gynaecol, 2004. 18(6): p. 827-42. PubMed PMID: 15582541. 5. Seale, P., A. Asakura, and M.A. Rudnicki, The potential...human fibroblasts by defined factors. Cell, 2007. 131(5): p. 861-72. PubMed PMID: 18035408. 10. Yu, J., et... the ectopic expression of four embryonic genes- OCT4, SOX2, KLF4, and C-MYC. iPSCs were first generated...communication and publishing after completing her postdoctoral training from Massachusetts General Hospital...

...modifications and homologous sequences are encoded at the 5’ end. First design the template binding region, with...insertion site. Encode half the new sequence at the 5’ end of each primer, with an overlap for homologous...primer by adding a short sequence complementary to the 5’ end of the reverse primer. Mutagenesis - Mutagenesis...codon and homologous region can be included at the 5’ end of either primer. Subcloning - To move a gene...recombination events to assemble the final plasmid. At least 5 fragments can be correctly assembled using IVA cloning...our Plasmids 101 blog posts Read our blog post on 5 factors to consider before choosing a cloning method...Laboratory of Molecular Biology. Jake Watson is a postdoctoral researcher in neurophysiology at the MRC Laboratory...

...postdocs had achieved tenure-track positions within 5-7 years of earning their PhDs (3), showing that the...reflect the number of positions available in academia (5, 6). Academic data and the labor market for PhDs Later...PMID: 25925677. Pubmed Central PMCID: PMC4484856. 5. Bourne, Henry R. "A fair deal for PhD students and...the Future of Research will be held from 22-24 October. This blog has been contributed by guest blogger...strong supporter of increasing the number of postdoctoral scientists at University of Maryland. Paula... can register here or live stream the event on October 22nd and 23rd here. Many thanks to our guest blogger...

... Cell 18, no. 5 (2016): 573-86. PubMed PMID: 27152442. PubMed Central PMCID: 4871596.  5. González, Federico...phosphatase treatment, substituting cytidine with 5-methylcytidine, and/or substituting uridine with pseudouridine...by the piggyBac transposon." Nature Methods 6, no. 5 (2009): 363-69. PubMed PMID: 19337237. PubMed Central...PubMed PMID: 20952534. PubMed Central PMCID: 2956203 5. Robinton, Daisy A., and George Q. Daley. "The promise...a 2A peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles...expression of endogenous pluripotency genes (i.e. Oct4) are other measures of reprogramming. If a retrovirus...plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28 Check out these plasmids if...

...phosphoserine directly into recombinant proteins (5). Optimization of this system defined three components...into recombinant proteins, such as the kinase MEK1 (5). MEK1 is inactive without requisite dual phosphorylation...307.5717 (2005): 1969-1972. PubMed PMID: 15790858. 5. Park, Hee-Sung, et al. "Expanding the genetic code...contributed by guest blogger Natalie Niemi, a postdoctoral fellow at the Morgridge Institute for Research...blogger Natalie Niemi! Natalie Niemi, Ph.D., is a postdoctoral fellow at the Morgridge Institute for Research...

...circumstance. For a more detailed discussion see [5]. Problems 1 and 2 depend on protein concentrations...PMID: 17586775. PubMed Central PMCID: PMC2206698. 5. Costantini, L.M., et al., Assessing the tendency ...oligomerize under physiologic conditions. Traffic, 2012. 13(5): p. 643-9. PubMed PMID: 22289035. PubMed Central ...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...

...your target gene! Tanenbaum et al., Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. Multi-bit genetic...create a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed.... Boncompain et al., Nat Methods 2012. Mar 11;9(5):493-8. MXS cloning platform for imaging and flow...for further chaining. The MXS-chaining kit contains 5 empty chaining vectors plus a destination vector and...half-life of both the luciferase mRNA (3’UTR AUUUA [5 consecutive]) and protein (C-terminal CL1 & PEST)....different promoters and/or allow for insertion at the 5′ or 3′ end of the sgRNA (i.e. TOP1 or TOP2 constructs...Translational Sequence (SITS). This sequence bypasses the 5′ mRNA cap required for eukaryotic systems and instead...

...dTAG-13 at doses as high as 20 µM (see Figures 3 and 5 in Nabet et al., Nature Chemical Biology for examples...degradation.. Nature Chemical Biology. May 2018. 14(5): 431-441. PubMed PMID: 29581585. This reference ...contributed by guest blogger Behnam Nabet, a postdoctoral fellow at Dana-Farber Cancer Institute. Targeted...studies (Nabet et al.).   Behnam Nabet, PhD is a postdoctoral fellow at the Dana-Farber Cancer Institute. ...

...WeShareScience is a free platform for sharing short, 3 to 5 minute videos about research. Videos can be shared... for communicating research. Short videos of 3 to 5 minutes provide more than enough time for researchers...measure the impact of their contribution. It offers a 5-step process and several examples for creating effective...Circadian Rhythm and Melatonin for the Timing of Nocturnal Courtship Vocalization. The video summarizes the...

...Cellular Biology 5(11): 3274–79. PubMed PMID: 2427927. PubMed Central PMCID: PMC369144. 5. Kranz, Andrea...Cellular Level in the Mouse.” Nature Biotechnology 21(5): 562–65. PubMed PMID: 12665802. 9. Villa, Katherine...guest blogger, Katrin Michel! Katrin Michel is a Postdoctoral Researcher at MIT and is fascinated by the question...

...consistent 5% positive error across the plate is fine; a 5% positive error on one side and a 5% negative... Probe is on a hairpin structure included in the 5’ primer Yes Yes. Agarose gel  LUX fluorogenic...experimentally.  Probe should have a melting temperature 5-10 degrees above the primer and be at least 25 bp ...Nucleic Acids Research, Volume 28, Issue 19, 1 October 2000, Pages 3752–3761, https://doi.org/10.1093/...

...methodology with T cell receptor engineering (Ref 5)  as described by Blue Flame Addgene depositor Professor...PMID: 26343581. PubMed Central PMCID: PMC4597191. 5. Eyquem, Justin, et al. "Targeting a CAR to the TRAC...Relationship” by our guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University! Cancer therapeutics...

...members to discuss your research plans for the next 5-10 years. You will discuss the focus of your first...for aficionados should be reserved for questions. 5. Understand the job you are applying for It's not ... potential collaborations, critiques of your postdoctoral research, etc. The second question is whether...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...and now serves as the Director of Student and Postdoctoral Programs at the Janelia Research Campus of the...

...job where people have regular schedules. Work 8 to 5 or 7 to 4 or maybe 7 to 6 and 8 to 10 or whatever ...before you get enmeshed in a serious relationship. #5 Thou shalt not sweat the small stuff The house can...And, yes, your partner CAN take the kids to the doctor without your help. Your partner is equally responsible...eight-year-old daughter vehemently reminded her doctor that she was allergic to penicillin – I am much...

...implants have been reported by several groups [4, 5, 6]. These have the obvious advantage of not requiring...PMID: 23580530; PubMed Central PMCID: PMC3769938. 5. Iwai Y, et al. A simple head-mountable LED device...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...

...the desired chromosomal modification can be made in 5 days. Counter-selection with sacB has revolutionized... editing in Escherichia coli." Scientific reports 5 (2015): 15096. PubMed PMID: 28060411. Thomason, Lynn...This post was contributed by Jacob Lazarus, a postdoctoral researcher at Harvard. There’s an astounding...is now an attending physician. He performs post-doctoral research in the lab of Matthew Waldor at Harvard...

... facilitate the isolation of modified cells; and (5) is compatible with the introduction of unique molecular... Find FITS plasmids here! Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard...

... Therapy Vol. 25 No 5. PubMed PMID: 28391961. PubMed Central PMCID: PMC5417792. 5. Naldini,L., 2015. Gene...amaurosis. This is the first of what I, scientists, doctors and patients hope are many more clinical successes...

...surgeries for viral injections can be found here [5, 6]. Opsin transgenic mice Transgenic mouse lines ...10.1038/nmeth.1808. PMID: 22179551; PMCID: PMC4165888. 5. Harris JA, et al. Adeno-associated viral vectors ...activation and silencing. Nature neuroscience. 2012;15(5):793-802. doi: 10.1038/nn.3078.  PMID: 22446880; PMCID...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...

...acquisition and retrieval of contextual fear memories [5].  In a modified behavioral paradigm, the authors ...primate cortex." Frontiers in systems neuroscience 5 (2011). PubMed PMID: 21811444. PubMed Central PMCID...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the...