... before being distributed to customers. Details about our production protocols, titering methods, and ...either in-house by Addgene scientists or through collaboration with viral vector manufacturing facilities, ...

...lightning speed to elucidate as much as they can about this novel virus. It is now known that SARS-CoV-...and open-access SARS-CoV-2 detection assay for laboratory and home testing. Kellner, et al. bioRxiv 2020.06.23.166397...

...Protocols gRNA Design Tools CRISPR Blog Posts In collaboration with the labs who have deposited CRISPR plasmids...short list of genes provided by the user. Read more about it on the Addgene blog . Eukaryotic Pathogen CRISPR...

... Feng Zhang's Lab, has assembled a list of FAQs about using the lab's CRISPR technology...., February 2013 ), has assembled a list of FAQs about using the lab's CRISPR technology. Visit the Zhang...

...serotype from the Alla Karpova and David Schaffer laboratories. These viral vector preparations were produced...Janelia Research Campus viral project team in collaboration with David Schaffer (University of California...

...systemic serotypes from the Viviana Gradinaru laboratory and the Caltech CLOVER Center (Link opens in ...AAV Decision Tree The AAV decision tree graphic above helps you choose the best capsid for your cell type...

...production. Read our AAV Guide for more information about: AAV components Common uses of AAV Viral integration...out our Viral Vector Packaging Service and learn about our options for hundreds of in-stock preps that ...

...article from Journal of Visualized Experiments (JoVE) about genomic deletions in mammalian cell lines using ...up to 20 μl. Use the primers designed in step 2 above. Conduct PCR for “non-deletion band” and “deletion...

... other are collaborating on projects. When I was a graduate student, I led a collaborative project between...funding and circumstances permit, think about having collaborators spend time with each other in person....meet up with collaborators. Being able to present data from multiple aspects of a collaboration and immediately...the Addgene blog Read our blog post about external collaborations Browse our science career related blog...communication a non-negotiable part of your collaboration. Luckily, there are techniques and tools that...same page. Ideally, these meetings include all collaborators, from the lab members who are conducting the...interesting results or ask questions to your collaborators. This helps everyone feel included in the project...

...guide: Collaborating with others outside your organization also known as external collaborations. Interestingly... and collaboration management. For example, you may already be part of a scientific collaboration - show...friendly, and helpful. Finally, don’t forget about collaborations and projects you can do with others after...lead! Read our blog post about fostering communication in remote collaborations! Stay in touch: Maintaining...notes that remind me about the current state of a partnership. Every collaboration and partnership is different...favored by research-intensive careers. External collaborations are becoming increasingly important as more...scientific project to the next level. External collaborations and partnerships are also very important in...

...particles that are suitable for in vivo use. Learn more about our viral service ID Viral Prep Description PI 65417...well as by our trusted partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity...

...use Twitter professionally to network, learn more about alternative careers for scientists, and share cool....” This paper addresses the following questions about scientists on Twitter: who are they? What do they...

...the Addgene Blog How did this change in labor laws come about? In March 2014, the White House issued a...must be above this level, and certain job duty tests must be met. In the Department of Labor’s general... to $47,476 per year, under updates to the Fair Labor Standards Act (FLSA). This is having a major effect...ensuring that either all affected scientists are paid above this threshold or for tracking the hours that these... a memorandum to the Secretary of Labor to update the FLSA, which had not seen revisions since 2004. In... In July 2015, the Department of Labor issued a Notice of Proposed Rulemaking, soliciting feedback by ...adjusted every 3 years. In May 2016, Secretary of Labor Thomas Perez updated the overtime regulations of...

... she spoke with Teytelman about an opportunity to work with a collaborative group, she also joined early...guide on Transferable Skills: External Collaborations Learn about the 2018 Minisymposium on Scientific ...organizations with similar ideas who were also thinking about reproducibility and shared similar desires to take...individuals with similar ideas to actually do something about it. Building a diverse team with different talents...the eLife ambassadors group was already thinking about ways to tackle irreproducibility. Benjamin Schwessinger...grassroots efforts. After thoughtful conversations about reproducibility with Teytelman, Schwessinger joined...previous work at Center for Open Science and Sense about Science. However, she realized that she couldn’t...

...else to focus on. Use collaborative cloud apps It is a good idea to use collaborative cloud tools such as...creative collaboration in the workplace. There are many other ways to encourage collaborations remotely...meeting? No problem! You can still creatively collaborate and brainstorm! Image from Joseph Mucira. ...serve as a springboard for future discussion. Collaboration and creativity are pivotal in writing publications...Vicens et al., 2007) geared towards enhancing collaborative writing and this recent article published in...approaches/tools we have to foster creative collaboration remotely in science are endless. Let’s adapt...blog post “Fostering Communication in Remote Collaborations” to get more ideas. Thank you to our guest...

...successes in metabolic engineering have been limited to the overexpression of various metabolites in Escherichia...species or metabolic pathways. A new study takes us one step closer to the vision of metabolic biofactories...cellular behaviors, such as differentiation or metabolism. Our attempts to manipulate these cellular behaviors...be host-independent, beyond the oft published metabolic engineering realm of S. cerevisiae and E. coli...control via scRNA design is readily apparent in metabolic engineering. The authors elegantly demonstrated...demonstrated the potential of this to control a metabolic network in S. cerevisiae by using three RNA programs ...programs, the authors sequentially rerouted the metabolic flux to generate differently pigmented derivatives...

... And what is ligase fidelity anyway? Let’s talk about it. DNA ligases are enzymes that seal breaks in ...to accelerate characterization of nucleic acid metabolic enzymes." Nucleic acids research 44.2 (2015): ...

...our blog post about remote collaborations Read our Transferable Skills Guide article about external collaborations...needed. In the example above, for example, we fostered a formal scientific collaboration between two research...project. We think that these collaborations add enormous value. A discussion about a protocol can save weeks..., the researchers did not have access to a P3 laboratory (BSL-3), a high-security facility needed to perform...approach was useful in humans. Then, one of them heard about our online platform, called Crowdfight, and filed...just the right infrastructure and offered to collaborate with them. The partnership was fruitful and a...This example clearly illustrates the power of collaboration, which despite the competitiveness of cutting-edge...

...Connie Cepko to learn about her mentoring style. Top 7 questions scientists ask me about mentoring In this... answers to the top questions scientists ask me about mentoring. Here they are: What makes a good Mentor... Where can I find a Mentor? What is this I hear about “group” or “peer” mentoring? How do I ask someone...a good science mentor? I started a conversation about many of these topics with the researchers who joined...often one that devotes time and energy to thinking about how to be a great Mentor.    Check Out Angela Depace's...questions in future posts. If you have a question about mentoring that you want us to cover, let us know...

...new conference rooms and learned some fun facts about these genes and the origins of their names!  ...