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We narrowed to 476 results for: FLU;

Showing: 41 - 60 of 476 results

  1. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ...that non-fluorescent tags may be advantageous over fluorescent proteins when the fluorescence is not essential...detection. Fluorescent proteins are most widely used tags because they emit endogenous fluorescence that allow...appropriate fluorescent illumination in in vivo, ex vivo, or post-mortem experiments. Fluorescent proteins...interest. Furthermore, fluorophore variants (for example green and red fluorescent proteins) allow simultaneous..., we found that fluorescent proteins might not always be the best option. Fluorescent proteins can interfere...techniques cannot be directly visualized with fluorescence or immunohistochemistry methods, these proteins...function Under some circumstances, the fused fluorescent proteins can be a roadblock, rather than an aid...

  2. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ... Posts on Fluorescent Proteins Browse Our Fluorescent Protein Pages Find the mOX Fluorescent Proteins ...protein non-fluorescent. Unfortunately, accumulated misfolded and nonfunctional (dark) fluorescent proteins...applicable to many microscopy experiments with fluorescent proteins (FPs) localized to compartments other...that they have missed the impact of dark, non-fluorescent, and misfolded FP-fusions on quantitative imaging... and cell physiology in general. Pitfalls of Fluorescent Protein Fusions Observing a bright signal for... fail to correctly fold? Misfolded FPs do not fluoresce. A dark population is not readily apparent and...investigate the localization and dynamics of fluorescent protein fusions in live cells in real time. Previously...

  3. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ...Updated May 10, 2021. In the world of fluorescent proteins and their use for imaging cell biology, Michael...searchable format on Addgene’s Michael Davidson Fluorescent Protein Collection webpage. These plasmids are... Over 300 empty backbones with 100+ distinct fluorophores to choose from in the green, red, yellow, cyan... 300 plasmid backbones spanning a rainbow of fluorescent proteins “It’s a great collection with over 300... the impressively big deposit. “He has every fluorescent protein under the sun, from the standard oldies...apple, papaya, and tomato) and the photoactivatable fluors (e.g. Phamret and Dendra). It’s just such a great...of interest isn’t in the ORF collection, the fluorescent proteins are, which makes mixing and matching...

  4. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal...levels and flux Nanosensor detection of an immunoregulatory tryptophan influx/kynurenine efflux cycle. PLoS...Measure autophagic flux by pH-sensitive fluorescence (pMRX-IP-GFP-LC3-RFP) An Autophagic Flux Probe that Releases...Reporters and Resources Fluorescent biosensors are proteins designed so that their fluorescent properties are ...signal of a circularly permuted fluorescent protein (cpFP) or fluorescence resonance energy transfer (FRET...orange-emitting fluorescent proteins. Nat Commun. 2017 Sep 5;8(1):431. Wolf Frommer Calcium Orange fluorescent calcium... Green fluorescence indicator of K+ for optical imaging (GINKO) Genetically encoded fluorescent indicators...

  5. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...oligomerization of fluorescent proteins is a concern. The propensity of fluorescent proteins to oligomerize...of the fluorescent protein are relatively close together (figure 1). Where should the fluorescent protein...length, the specific fluorescent protein variant, and where to add the fluorescent tag. These choices will...dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins." Proceedings...Addgene blog Read our fluorescent proteins blog posts Download the fluorescent proteins 101 eBook Read....org Browse the fluorescent protein collection Find empty backbones for fluorescent protein fusions Browse...Amsterdam). Tagging a protein of interest with a fluorescent protein to study its function is one of the most...

  6. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...photostable fluorophores mOrange2, EGFP, and mKate2 (red) to solve the problematically low fluorescent intensity...default, they are not fluorescent. Instead, they incorporate a truncated, non-fluorescent form of YFP as the...is necessary to excise the non-fluorescent YFP and activate fluorescence of the downstream OFP, GFP, or...combination of all of the fluorescent proteins from the injected construct. The fluorescent protein expressed ...upstream of the fluorescent protein genes, Cre recombination is required before fluorescence can occur. Cre... be labeled by synthetic fluorophores, which are more stable than fluorescent proteins. For more details...Visit our fluorescent proteins topic page Resources on Addgene.org Browse Addgene’s fluorescent protein...

  7. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    ...Their Fluorescence fLuctuation INcrease by Contact (FLINC)-based biosensors measure fluctuations of TagRFP-T...TagRFP-T fluorescence intensity due to interactions with photoactivatable fluorescent protein Dronpa. ...derivatives encoding alternative promoters and/or fluorescent tags are available at Addgene. Shemesh OA, ...proximity. By monitoring changes in TagRFP-T fluorescence intensity at different time points, the authors... Methods. 2017. PubMed PMID: 28288122. New fluorescent proteins for use as FLIM-FRET acceptors  Listen...-FRET acceptors, ShadowG, a novel dark green fluorescent protein (GFP) with an absorption peak of 486 ...486 nm and ShadowY, a novel dark yellow fluorescent protein (YFP) with an excitation peak of 519 nm. These...

  8. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Plasmid Collections Fluorescent Proteins Michael Davidson Fluorescent Protein Collection Michael Davidson...Find fluorescent protein fusion constructs and cloning backbones from the Michael Davidson Lab. Plasmid...Davidson Fluorescent Protein Collection Empty Backbones Blue Cyan Green mEmerald Yellow Orange Red Photoactivatable... collection of ORFs tagged with a variety of fluorescent proteins. The majority of the ORFs are of mammalian... corresponding empty backbone with different fluorescent tags for you to create fusion proteins with your...

  9. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ... Plasmid Collections Fluorescent Proteins In Vivo Imaging Fluorescent Proteins...photoactivatable fluorescent proteins. Plasmid...organs and whole mammals. Genetically encoded fluorescent proteins most suitable for stable imaging of ...undesirable background signals. Many near-infrared fluorescent proteins for in vivo imaging are derived from... photoreceptors (BphPs). Spectrally distinct fluorescent iRFP variants have been shown to have high brightness...of the original iRFP has resulted in a split fluorescence complementation probe used for detecting protein-protein...

  10. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... Plasmid Collections Fluorescent Proteins Subcellular Localization Fluorescent Proteins: Subcellular ... protein to a red fluorescent protein (find plasmids for cloning in our Fluorescent Proteins Empty Backbones...Collection Michael Davidson Fluorescent Protein Collection Addgene Blog: Fluorescent Proteins Do you have suggestions...Find plasmids encoding fluorescent fusion proteins with known subcellular localization patterns for colocalization...localization. One of the most common is to use fluorescence microscopy to compare the localization of your...trafficking. Addgene has assembled a collection of fluorescent fusion proteins that are targeted to various ... should fuse your protein of interest with a fluorescent protein that has a different emission wavelength...

  11. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Return to top Fluorescent Timers Fluorescent protein variants that change their fluorescence from the blue... Plasmid Collections Fluorescent Proteins Empty Backbones Fluorescent Proteins: Empty Backbones ...Collection Michael Davidson Fluorescent Protein Collection Botman-Teusink Yeast Fluorescent Protein Collection ...Addgene Blog: Which Fluorescent Protein Should I Use? Addgene Blog: Choosing Your Fluorescent Proteins for Multi-Color...Explore empty plasmid backbones with different fluorescent tags to create fusion proteins with your gene...Resources The following tables list many popular fluorescent proteins and empty backbones for expression and...experiment. Tables are organized by color of the fluorescent protein. The content for this page was originally...

  12. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ...PPIs): Bimolecular Fluorescence Complementation (BiFC). What is bimolecular fluorescence complementation?...BiFC only uses a single fluorescent protein to visualize PPIs. This fluorescent protein (FP) is split into...complete FP results in a fluorescent signal that can be detected by fluorescence microscopy. Figure..., resulting in a fluorescent readout.   Applications for bimolecular fluorescence complementation BiFC...non-radiative transfer for energy between neighboring fluorophores, FRET can be used to detect the inter- and intramolecular...complementation? Bimolecular fluorescence complementation is a method for detecting protein-protein interactions that...that uses split fluorescent proteins tethered to potential protein interaction partners. Unlike FRET, BiFC...

  13. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    ... reduced eGFP fluorescence. Cells expressing the library are then sorted by fluorescence-activated cell... to use a suite of fluorescent reporters. Bacteria containing unstable fluorescent proteins under the ...inoculation, no more fluorescent proteins are made. As the bacterial replicate, fluorescent proteins will be...Davidson and Roger Tsien played enormous roles in fluorescent protein development by openly sharing their resources... a conference of their choice to share their fluorescent protein based research. This year, we put out...were excited to see the many creative uses of fluorescent proteins. We’re happy to announce the three winners...levels of abundance, the eGFP will be stable and fluoresce. But if the variant is unstable, it will be degraded...

  14. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    ... in increased fluorescent signal. Prior to the development of these probes, fluorophore binding aptamers...cobalamin-binding riboswitches that bind to cobalamin-fused fluorescent dyes; binding of the cobalamin fusion to the ...aptamers and MS2-binding fluorescent proteins were the state of the art in RNA imaging in vivo. These new ...aptamers or MS2 tags using the same number of fluorophores. You can find plasmids containing the cobalamin...are always on the lookout for better, brighter fluorescent proteins (FP) with improved tissue penetrance...30220597. ACE system: A versatile in vivo fluorescent reporter for rapid quantification of base-editing...deposited a reporter plasmid that allows for rapid fluorescence-based quantification of base editors that utilize...

  15. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    ...artifacts. If you're using green fluorescent protein or Enhanced Green Fluorescent Protein (GFP/EGFP) for a transcriptional... means that your POI fused to GFP or another fluorescent protein (FP) could be forming dimers in cells... Figure 1: Each problem with dimerizing fluorescent proteins is illustrated. 1) Membrane proteins... by FP dimerization.   1. Transmembrane fluorescent protein fusions Transmembrane proteins, i.e. ...energy to the second FP, often decreasing the fluorescent signal from the first FP and increasing the signal...These simple strategies can help you make better fluorescent fusion protein reporters for your studies and...PubMed PMID: 11988576. 3. Matz, M.V., et al., Fluorescent proteins from nonbioluminescent Anthozoa species...

  16. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    ...minutes of inactivating the CASANOVA complex, fluorescent CRISPR complexes were observed binding telomeric...30377362. Studying neuronal activity using the fluorescent voltage indicator Archon Article contributed ...team identified Archon1, a novel opsin-based fluorescent voltage indicator. Archon1 can embed itself into...Heckler et al., 2015) or ratiometric Bimolecular Fluorescence Complementation (rBiFC, Grefen and Blatt, 2012...adapted cells expressing these plasmids, tgRFP fluorescence localizes to the mitochondria, because cPYP ...

  20. Immunocytochemistry

    Type
    Protocol
    ...primary antibody against a target protein and a fluorescent secondary antibody....primary antibody against a target protein and a fluorescent secondary antibody. This protocol outlines the... tips and pipettes Rocking platform Tweezers Fluorescent microscope 0.5–10 µL single channel pipette 2...500 µL PBS on a rocking platform. Dilute the fluorescently-labeled secondary antibody to the desired concentration...generally ranges from 1-10 µg/mL. Add 500 µL fluorescently-labeled secondary antibody to the wells and ...cell labeling on a microscope with appropriate fluorescent filters. Tips and Troubleshooting The optimal...
Showing: 41 - 60 of 476 results