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We narrowed to 472 results for: FLU;

Showing: 41 - 60 of 472 results

  1. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    Blog Post
    ...that non-fluorescent tags may be advantageous over fluorescent proteins when the fluorescence is not essential...detection. Fluorescent proteins are most widely used tags because they emit endogenous fluorescence that allow...appropriate fluorescent illumination in in vivo, ex vivo, or post-mortem experiments. Fluorescent proteins...interest. Furthermore, fluorophore variants (for example green and red fluorescent proteins) allow simultaneous..., we found that fluorescent proteins might not always be the best option. Fluorescent proteins can interfere...techniques cannot be directly visualized with fluorescence or immunohistochemistry methods, these proteins...function Under some circumstances, the fused fluorescent proteins can be a roadblock, rather than an aid...

  2. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ... a synthetic, fluorescent analogue have been engineered (Thorn, 2017). These fluorescent analogues are...of the available green fluorescent proteins emit light. The pKa of fluorescent proteins can be accurately...locations (Chen et al., 2013). GFP fluorescence overlaps with autofluorescence The cyan light that is used to...the use of bright red fluorescent proteins (e.g. mScarlet) or infrared fluorescent proteins (Chernov et...Introduction to GFP Read our Fluorescent Proteins Blog Posts Download the Fluorescent Proteins 101 eBook Resources....org Browse the Fluorescent Protein Collection Find empty backbones for fluorescent protein fusions Browse...popular, most widely used genetically encoded fluorescent probe. Several factors contribute to the popularity...

  3. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    Blog Post
    ... Posts on Fluorescent Proteins Browse Our Fluorescent Protein Pages Find the mOX Fluorescent Proteins ...protein non-fluorescent. Unfortunately, accumulated misfolded and nonfunctional (dark) fluorescent proteins...applicable to many microscopy experiments with fluorescent proteins (FPs) localized to compartments other...that they have missed the impact of dark, non-fluorescent, and misfolded FP-fusions on quantitative imaging... and cell physiology in general. Pitfalls of Fluorescent Protein Fusions Observing a bright signal for... fail to correctly fold? Misfolded FPs do not fluoresce. A dark population is not readily apparent and...investigate the localization and dynamics of fluorescent protein fusions in live cells in real time. Previously...

  4. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

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    Blog Post
    ...Updated May 10, 2021. In the world of fluorescent proteins and their use for imaging cell biology, Michael...searchable format on Addgene’s Michael Davidson Fluorescent Protein Collection webpage. These plasmids are... Over 300 empty backbones with 100+ distinct fluorophores to choose from in the green, red, yellow, cyan... 300 plasmid backbones spanning a rainbow of fluorescent proteins “It’s a great collection with over 300... the impressively big deposit. “He has every fluorescent protein under the sun, from the standard oldies...apple, papaya, and tomato) and the photoactivatable fluors (e.g. Phamret and Dendra). It’s just such a great...of interest isn’t in the ORF collection, the fluorescent proteins are, which makes mixing and matching...

  5. Fluorescent Protein Guide: Activity Regulation

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    Collection
    ... Plasmid Collections Fluorescent Proteins Activity Regulation Fluorescent Proteins: Activity Regulation...Plasmids using a fluorescent protein as a scaffold to allow scientists to selectively modulate gene expression...Regulation More Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Biosensors Optogenetics... Optogenetics Background Fluorescent proteins such as GFP have long been used for labeling, but new methods...methods are emerging to use these fluorescent proteins to modulate biological activity. Early tools allow...GFP-lines for cell-specific manipulation. Plasmids Fluorescent Protein Purpose Principal Investigator Plasmids...

  6. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Proteins Michael Davidson Fluorescent Protein Collection Michael Davidson Fluorescent Protein Collection More...Davidson lab fluorescent protein fusion constructs and backbones. Plasmid... Plasmid Collections Fluorescent Proteins...More Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics The lab ... collection of ORFs tagged with a variety of fluorescent proteins. The majority of the ORFs are of mammalian... corresponding empty backbone with different fluorescent tags for you to create fusion proteins with your...

  7. Fluorescent Protein Guide: In Vivo Imaging

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    Collection
    ...Imaging Guide Fluorescent Proteins: In Vivo Imaging More Fluorescent Protein Resources: Empty Backbones FRET...near-infrared, and photoactivatable fluorescent proteins. Plasmid... Plasmid Collections Fluorescent Proteins In Vivo Imaging...organs and whole mammals. Genetically encoded fluorescent probes are suitable for stable imaging of protein...undesirable background signals. Near-infrared fluorescent proteins for in vivo imaging are derived from...photoreceptors (BphPs). Spectrally distinct permanently fluorescent iRFP variants have been shown to have high effective...of the original iRFP has resulted in a split fluorescence complementation probe used for detecting protein-protein...

  8. Fluorescent Protein Guide: Subcellular Localization

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    Collection
    ... Plasmid Collections Fluorescent Proteins Organelle Markers Fluorescent Proteins: Subcellular Localization...Plasmids encoding fluorescent proteins tagged with genes or peptides with known subcellular localization...Localization More Fluorescent Protein Resources: Empty Backbones Allen Institute for Cell Science Plasmid...Plasmid Collection FRET Biosensors Optogenetics Fluorescent Protein Blog Posts Background General Cytoskeletal...localization. One of the most common is to use fluorescence microscopy to compare the localization of your...markers. Addgene has assembled a collection of fluorescent fusion proteins that are targeted to various ... should fuse your protein of interest with a fluorescent protein that has a different emission wavelength...

  9. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... Special Collections Fluorescent Protein Guide Empty Backbones Fluorescent Protein Guide: Empty Backbones...which fluorescence intensity is 50% of its maximum value. Maturation is the time for fluorescence to obtain...collection of empty plasmid backbones with different fluorescent tags for you to create fusion proteins with your...Backbones More Fluorescent Protein Resources: FRET Biosensors Subcellular localization Michael Davidson...Multi-Color Imaging Blog: Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Blog: When is a ...a Monomer not a Monomer? Background Fluorescent proteins are genetically encoded tools that are used extensively...extensively by life scientists. The original green fluorescent protein (GFP) was cloned in 1992 ( Prasher et...

  10. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...oligomerization of fluorescent proteins is a concern. The propensity of fluorescent proteins to oligomerize...of the fluorescent protein are relatively close together (figure 1). Where should the fluorescent protein...length, the specific fluorescent protein variant, and where to add the fluorescent tag. These choices will...dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent proteins." Proceedings...Addgene blog Read our fluorescent proteins blog posts Download the fluorescent proteins 101 eBook Read....org Browse the fluorescent protein collection Find empty backbones for fluorescent protein fusions Browse...Amsterdam). Tagging a protein of interest with a fluorescent protein to study its function is one of the most...

  11. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...photostable fluorophores mOrange2, EGFP, and mKate2 (red) to solve the problematically low fluorescent intensity...default, they are not fluorescent. Instead, they incorporate a truncated, non-fluorescent form of YFP as the...is necessary to excise the non-fluorescent YFP and activate fluorescence of the downstream OFP, GFP, or...combination of all of the fluorescent proteins from the injected construct. The fluorescent protein expressed ...upstream of the fluorescent protein genes, Cre recombination is required before fluorescence can occur. Cre... be labeled by synthetic fluorophores, which are more stable than fluorescent proteins. For more details...Visit our fluorescent proteins topic page Resources on Addgene.org Browse Addgene’s fluorescent protein...

  12. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    ...Their Fluorescence fLuctuation INcrease by Contact (FLINC)-based biosensors measure fluctuations of TagRFP-T...TagRFP-T fluorescence intensity due to interactions with photoactivatable fluorescent protein Dronpa. ...derivatives encoding alternative promoters and/or fluorescent tags are available at Addgene. Shemesh OA, ...proximity. By monitoring changes in TagRFP-T fluorescence intensity at different time points, the authors... Methods. 2017. PubMed PMID: 28288122. New fluorescent proteins for use as FLIM-FRET acceptors  Listen...-FRET acceptors, ShadowG, a novel dark green fluorescent protein (GFP) with an absorption peak of 486 ...486 nm and ShadowY, a novel dark yellow fluorescent protein (YFP) with an excitation peak of 519 nm. These...

  13. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ...PPIs): Bimolecular Fluorescence Complementation (BiFC). What is bimolecular fluorescence complementation?...BiFC only uses a single fluorescent protein to visualize PPIs. This fluorescent protein (FP) is split into...complete FP results in a fluorescent signal that can be detected by fluorescence microscopy. Figure..., resulting in a fluorescent readout.   Applications for bimolecular fluorescence complementation BiFC...non-radiative transfer for energy between neighboring fluorophores, FRET can be used to detect the inter- and intramolecular...complementation? Bimolecular fluorescence complementation is a method for detecting protein-protein interactions that...that uses split fluorescent proteins tethered to potential protein interaction partners. Unlike FRET, BiFC...

  14. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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    Blog Post
    ... reduced eGFP fluorescence. Cells expressing the library are then sorted by fluorescence-activated cell... to use a suite of fluorescent reporters. Bacteria containing unstable fluorescent proteins under the ...inoculation, no more fluorescent proteins are made. As the bacterial replicate, fluorescent proteins will be...Davidson and Roger Tsien played enormous roles in fluorescent protein development by openly sharing their resources... a conference of their choice to share their fluorescent protein based research. This year, we put out...were excited to see the many creative uses of fluorescent proteins. We’re happy to announce the three winners...levels of abundance, the eGFP will be stable and fluoresce. But if the variant is unstable, it will be degraded...

  15. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    ... in increased fluorescent signal. Prior to the development of these probes, fluorophore binding aptamers...cobalamin-binding riboswitches that bind to cobalamin-fused fluorescent dyes; binding of the cobalamin fusion to the ...aptamers and MS2-binding fluorescent proteins were the state of the art in RNA imaging in vivo. These new ...aptamers or MS2 tags using the same number of fluorophores. You can find plasmids containing the cobalamin...are always on the lookout for better, brighter fluorescent proteins (FP) with improved tissue penetrance...30220597. ACE system: A versatile in vivo fluorescent reporter for rapid quantification of base-editing...deposited a reporter plasmid that allows for rapid fluorescence-based quantification of base editors that utilize...

  16. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    ...artifacts. If you're using green fluorescent protein or Enhanced Green Fluorescent Protein (GFP/EGFP) for a transcriptional... means that your POI fused to GFP or another fluorescent protein (FP) could be forming dimers in cells... Figure 1: Each problem with dimerizing fluorescent proteins is illustrated. 1) Membrane proteins... by FP dimerization.   1. Transmembrane fluorescent protein fusions Transmembrane proteins, i.e. ...energy to the second FP, often decreasing the fluorescent signal from the first FP and increasing the signal...These simple strategies can help you make better fluorescent fusion protein reporters for your studies and...PubMed PMID: 11988576. 3. Matz, M.V., et al., Fluorescent proteins from nonbioluminescent Anthozoa species...

  17. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    ...minutes of inactivating the CASANOVA complex, fluorescent CRISPR complexes were observed binding telomeric...30377362. Studying neuronal activity using the fluorescent voltage indicator Archon Article contributed ...team identified Archon1, a novel opsin-based fluorescent voltage indicator. Archon1 can embed itself into...Heckler et al., 2015) or ratiometric Bimolecular Fluorescence Complementation (rBiFC, Grefen and Blatt, 2012...adapted cells expressing these plasmids, tgRFP fluorescence localizes to the mitochondria, because cPYP ...
Showing: 41 - 60 of 472 results