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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 974 results for: LAT

Showing: 41 - 60 of 974 results

  1. Ras Pathway

    Type
    Collection
    ...(ras related) RALBP1 RalA binding protein 1 RALGDS Ral guanine nucleotide dissociation stimulator RAPGEF...have an active (GTP) and inactive (GDP) state, regulated by the addition and removal of a phosphate group...GTPases play prominent roles in signaling pathways regulating cell migration, survival, adhesion, growth, and...differentiation and survival. Ras is essential for regulating the growth of the cell, however when mutations...for clarity and does not indicate a specific relationship. The content and map for this page were generated...EIF4EBP EIF4EBP1 EIF4EBP2 EIF4EBP3 Eukaryotic translation initiation factor 4E binding protein ERBB2 Erb-b2...

  2. Brain Initiative Collection

    Type
    Collection
    ...cortical interneurons under the control of the E2 regulatory element Jordane Dimidschstein 135630-AAV9 pAAV-S5E2...cortical interneurons under the control of the E2 regulatory element Jordane Dimidschstein 135630-PHPeB pAAV-S5E2...cortical interneurons under the control of the E2 regulatory element Jordane Dimidschstein 135631-AAV1 pAAV-S5E2...cortical interneuronsunder the control of the E2 regulatory element Jordane Dimidschstein 135631-AAV9 pAAV-S5E2...cortical interneuronsunder the control of the E2 regulatory element Jordane Dimidschstein 135631-PHPeB pAAV-S5E2...cortical interneuronsunder the control of the E2 regulatory element Jordane Dimidschstein 135632-AAV1 pAAV-S5E2...cortical interneuronsunder the control of the E2 regulatory element Jordane Dimidschstein 135632-AAV9 pAAV-S5E2...

  3. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...include histone acetylation by p300, histone demethylation by LSD1, cytosine methylation by DNMT3A or MQ1...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ...100 bp window. This technique creates diverse populations of mutants for directed evolution. ID Plasmid...Nickase mutants can also be used with a repair template to introduce specific edits via homology-directed...primer binding site and the desired edits on an RT template. ID Plasmid Gene/Insert Promoter Selectable Marker...

  4. Zebrafish Plasmid Collection

    Type
    Collection
    ...NTR 2.0/MTZ-based cell ablation system that allows sustained cell loss and ablation of previously...localization in high-throughput. MAZERATI platform - Leonard Zon Lab. A platform to rapidly model potential cancer...embryo and larvae, it is easy to culture large populations that are amenable to genetics, high-throughput...emerged as a model system for human disease and translational research, including the study of cancer, inflammation... enabling CRISPR/dCas9-interference (CRISPRi) modulation of enhancers and non-coding RNAs in zebrafish...organism lineage tracing by combinatorial and cumulative genome editing - Jay Shendure Lab Simultaneous...droplet reporters - Richard White Lab. An imaging platform to study lipid dynamics in vivo. Bimolecular fluorescence...

  5. Rett Syndrome

    Type
    Collection
    ... of treatments and cures for Rett Syndrome and related MECP2 disorders. The RSRT has a comprehensive, ...diagnostic criteria, defined as the presence of relatively normal early development, regression with a loss...The MECP2 protein is a global transcriptional regulator of thousands of genes and studies have suggested...is not clear, however, the ability to bind to methylated DNA and recruit known co-repressors or other ...modifiers suggests the major function of MECP2 is to regulate gene expression either locally or globally. The...NID demonstrating the importance of binding to methylated DNA and the recruitment of the NCoR complex for... MECP2 null alleles or common mutations that recapitulate Rett syndrome in humans. MECP2 knockout rats...

  6. p53 Pathway

    Type
    Collection
    ...through both phosphorylation and acetylation, which disrupt its interaction with negative regulators, increase...Caspase 3, apoptosis-related cysteine peptidase CASP8 Caspase 8, apoptosis-related cysteine peptidase CASP9...allow it to bind transcriptional co-activators and modulate transcription. The list of p53 transcriptional...for clarity and does not indicate a specific relationship. p53 Pathway Color is used for clarity and does... does not indicate a specific relationship. The content for this page was generated with the help of .... CASP9 Caspase 9, apoptosis-related cysteine peptidase Cyclin B CCNB1 CCNB2 CCNB3 Cyclin B1, 2, or 3 Cyclin...proto-oncogene, E3 ubiquitin protein ligase MDMX p53 regulator; also known as MDM4 Noxa Phorbol-12-myristate-...

  7. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ... a variety of cell populations in different brain regions. Labeled cell populations were tested in mouse...for cell populations in the following regions: Isocortex Hippocampal formation Cortical subplate Striatum...AiP20013 AiE2674m SYFP2 Entorhinal area, lateral part - L2 stellate neurons Hippocampal formation 214479 ...labeling and recombination across brain regions and populations created by the Allen Institute for Brain Science...Enhancer AAV plasmids are plasmids which use genomic regulatory sequences — termed enhancers — to drive cell ...fluorescent labeling across brain regions and populations using systemic AAV delivery. The collection includes...Institute ID Enhancer ID FP/Recombinase Labeled Population Region 230551 pAAV-AiE2638m-minBG-SYFP2-WPRE3...

  8. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...interneurons under the control of the E2 regulatory element Viral manipulation of functionally distinct interneurons...calcium sensors for imaging activity in neuronal populations and microcompartments. Nat Methods. 2019 Jul;...GCaMP6f Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator. Neuron... enhancer A viral strategy for targeting and manipulating interneurons across vertebrate species. Nat ...) Improved methods for marking active neuron populations. Nat Commun. 2018 Oct 25;9(1):4440. Eric Schreiter...ER-targeted CEPIA1er ER-mitochondria tethering by PDZD8 regulates Ca(2+) dynamics in mammalian neurons. Science....sensitivity Sensitive genetically encoded sensors for population and subcellular imaging of cAMP in vivo. Nat ...

  9. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... cell culture. Five years later, PubMed lists more than 6,300 CRISPR-related publications, many of which...specific, programmable platform well-suited for various biological and translational technologies. Biotechnology... a target gene. On the other hand, if a repair template with homology arms was supplied, the break could...could be repaired according to this template, allowing for precise gene editing. These edits can include...convert adenosine to inosine on RNA. Inosine is translated as guanosine, so the overall effect is an A->...to transcriptional activators or repressors can modulate transcription in a reversible manner. Epigenetics...Epigenetics : dCas9 fused to epigenetic modifiers can modulate transcriptions and create heritable epigenetic...

  10. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...including histone acetylation/demethylation, and cytosine methylation/demethylation....include: histone acetylation by p300 histone demethylation by LSD1 cytosine methylation by DNMT3A or MQ1...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ...MQ1 cytosine demethylation by Tet1 These modifications persist over time and are potentially heritable...

  11. Deisseroth INTRSECT Collection

    Type
    Collection
    ...viral expression of molecular payloads in target populations defined by multiple parameters....expressing these tools in well-defined cellular sub-populations has generally been limited to single-component...viral expression of molecular payloads in target populations defined by multiple parameters. Figure 1: Examples...Examples of intersectional cell population definitions using recombinases. A) Cre and Flp are multiplexed... to enable intersectional viral targeting of populations expressing only Cre AND Flp, Cre NOT Flp, or ...recombinase-expression strategies to define cellular sub-populations of interest, including dual-transgenic recombinase-expressing... NOT Cre No Dual recombinase-dependent: Rabies-related Addgene ID Plasmid Logic Sites and Mutations In-Stock...

  12. Viral Production

    Type
    Collection
    ...cells several days later, and isolating genomic DNA. This DNA is then used as a template to amplify a portion...Next-generation sequencing is performed on viral genomes isolated from the final AAV preparation. Sequencing results...of AAV preparations is assayed by comparing the relative stoichiometric ratios of the viral capsid proteins...SYPRO Ruby staining and the molecular weight and relative intensity of the viral capsid proteins are analyzed...Viral capsid proteins VP1, VP2, and VP3 are shown relative to the total protein present in the sample. Sterility...are added to cells in culture. Three to five days later, cell cultures are inspected for sterility. Transducibility...-AAVrg) alone at 1.1E6 vg/mL, or both. Two days later, Cre-dependent GFP expression was detected with ...

  13. Neurodegeneration Research Collection

    Type
    Collection
    ...available at Addgene. Amyotrophic Lateral Sclerosis Amyotrophic lateral sclerosis (ALS), sometimes referred...individuals develop late-onset AD. There is no clear association between a gene and late-onset AD. It is ... between a person’s thirties and mid-sixties, or late-onset, where symptoms appear during or after a person's...APOE), such as the ε4 allele, are a risk factor for late-onset AD. Many more genes have been associated with...reporter to study tau seeding in mammalian cells. Lathuiliere et al. Mol Neurodegener. 2023 Aug 8. Study the...growing collection of general and neuroscience -related antibodies including: Anti-KChIP1 K+ channel [K55...high-volume hybridoma sequencing on the NeuroMabSeq platform. Mitchell et al. Sci Rep. 2023 Sep 27. See More...

  14. Tetracycline Inducible Expression

    Type
    Collection
    ...Learn about tetracycline-regulated gene expression and find plasmids for your experiments using tet- ...view highlighted Tet plasmids . Figure 1: Tet-regulated expression systems. Left: natural TetR mechanism...transduced cells express both components in the same relative ratio. Different generations of transactivators...of background expression. For example, only a relatively low level of doxycycline can access the brain...toxicity, a known half-life (24 hours), and is relatively inexpensive. The induction profiles for dox and...applications, or search our collection for all dox-regulated plasmids . Looking for viral preps? Find in-stock... systems or search our collection for all dox-regulated plasmids . ID Plasmid Description PI 63800 PB-...

  15. Synthetic Biology - Overview

    Type
    Collection
    ...plasmids related to metabolic pathways and auxiliary components. Networks and Gene Regulation Browse plasmids...Lab Biopixels Jaramillo Lab Riboregulation Keasling Lab BglBrick Platform , Terpenoid Production , and...Category Cloning and Genomic Tools Browse plasmids related to cloning and genomic modification, including ...containing both naturally occurring and synthetic regulatory elements, including promoters, terminators, repressors...networks. Sensing and Signaling Browse plasmids related to intercellular signaling and environmental sensing...Plasmids and Phagemids Balazsi Lab Negative Autoregulation and Yeast-Mammalian Circuit Transfer C. Collins...

  16. COVID-19 Resources

    Type
    Collection
    ...the knowledge and understanding of COVID-19 and related coronaviruses. To assist with this effort Addgene...plasmids available that may be useful for COVID-19 related research. Additionally, we have linked to collections...articles, protocols, and other resource collections related to COVID-19 that may be of use to scientists. Background...Description Industry PI Due to export control regulations, MERS and SARS-CoV materials are available only...entry of SARS-CoV and SARS-CoV-2. Find plasmids related to these genes below. ID Plasmid Description Industry... in the body. For a full list of cytokines and related plasmids see our Immunology Resource Guide . Return...and DETECTR - Simple, portable, and inexpensive platforms to reliably detect nucleic acids at the atomolar...

  17. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and ...edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed...Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting ...Nickase mutants can also be used with a repair template to introduce specific edits via homology-directed...primer binding site and the desired edits on an RT template. ID Plasmid Gene/Insert Promoter PI Publication... to direct the dCas9-activator to promoter or regulatory regions of your gene of interest. If the plasmid...the spread of infection throughout the entire population. ID Plasmid Promoter PI Publication Purify A ...

  18. Zhang Lab CRISPR Page

    Type
    Collection
    ...and constitutively expressing Cas9 knockin mice (Platt et al ., Cell 2014). In these mice the CRISPR-Cas9...luciferase-2A-Cre recombinase, and Kras G12D HDR template 60225 : control for AAV-KPL; sgRNA targeted to...expression vectors for cancer modeling Using Cas9 mice, Platt et al . simultaneously modeled the dynamics of KRAS...contains a Kras G12D homology directed repair donor template. #60225 - AAV:ITR-U6-sgRNA(LacZ)-pEFS-Rluc-2A-...to enable the rapid ligation of annealed and phosphorylated oligos designed based on the target site sequence...for genome editing in the brain Using Cas9 mice Platt et al. demonstrated in vivo genome editing in the...

  19. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...sequence (not shown). (B) Relative GFP and RFP concentration given relatively to the non‐targeting spacer...the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently...has deposited a collection of CRISPR arrays to modulate the expression levels of two targets independently...sb400021j Figure 1: Multiplexing CRISPR knockdown to modulate expression of two genes without cross‐talk. (A... a single cell, and each color represents the population obtained with one CRISPR plasmid. An overlaid... connects the median values of the different populations. Image and caption from Vigouroux et al., 2018...

  20. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...powerful genome engineering tools used to precisely manipulate DNA and control the expression of specific genes...Dre with rox sites), enabling precise genetic manipulation without cross-reactivity with other recombinase... in the presence of tamoxifen. A tetracycline-regulated or other drug-inducible approach may also be used...Genes A and B. When Cre is absent, only Gene A is translated correctly. Cre expression excises Gene A and ...and alters the reading frame to allow in-frame translation of Gene B. FLEx Switch (for “flip-excision”):...available, both mutant and wild type. The ability to manipulate the number, orientation, and type of target sites...can improve specificity in targeted neuronal populations. Read our blog post about how to minimize leakiness...
Showing: 41 - 60 of 974 results