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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 771 results for: LIS

Showing: 41 - 60 of 771 results

  1. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...Central PMCID: PMC4461904. 9. Huang, Q., Mao, Z., Li, S., Hu, J., & Zhu, Y. “A non-radioactive method ...PMC164612. 11. Liou, Y.-R., Wang, Y.-H., Lee, C.-Y., & Li, P.-C. “Buoyancy-Activated Cell Sorting Using Targeted...short list of some other common applications and links to relevant papers and webpages are listed below...such as gluconeogenesis, lipogenesis, amino acid metabolism and energy transduction. Biotinylation is the... EMSA (Electrophoretic Mobility Shift Assays) ELISA (Enzyme-Linked Immunosorbent Assay) Southern/Northern... Krijgsveld, J., Katsantoni, E., Heck, A., … Strouboulis, J. “Efficient biotinylation and single-step ...

  3. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...Zhang X, Su J, Jeong M, Gundry MC, Huang Y-H, Zhou Y, Li W, Goodell MA (2017) Targeted DNA methylation in ...publication at Addgene. Xu X, Tao Y, Gao X, Zhang L, Li X, Zou W, Ruan K, Wang F, Xu G, Hu R (2016) A CRISPR-based...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...are also available as controls. For an up-to-date list of CRISPR epigenetic tools, check out our CRISPR...

  4. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...doi.org/10.1104/pp.15.00636 Ma X, Zhang X, Liu H, Li Z (2020) Highly efficient DNA-free plant genome editing... . https://doi.org/10.1073/pnas.1818290116 Zhu H, Li C, Gao C (2020) Applications of CRISPR–Cas in agriculture...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...

  5. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...prep pAAV-hSyn-GRAB-gDA3m AAV1 Biosensors Yulong Li New viral prep pGP-AAV-GFAP-iGABASnFR2-WPRE AAV1...

  6. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ..., Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park J, Blackburn EH, Weissman JS, Qi LS, Huang...that there’s a new CRISPR advance or technique published every week! One of the newest applications is ...fluorescent distances correlating with the previously established physical map. In comparing pairs of targets ~...

  8. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ..., (Constitutive or Cre-dependent) Li Lab GRAB_Ado Plasmids Yulong Li ADP A fluorescent, reagentless biosensor.... 2021 Dec 17. pii: S0896-6273(21)00988-0. Yulong Li ATP/ADP Monitor the ATP:ADP ratio during live-cell...studies. Nat Biotechnol. 2018 Sep;36(8):726-737. Yulong Li Acetylcholine Optimized acetylcholine sensor for ...2020 Sep 28. pii: 10.1038/s41592-020-0953-2. Yulong Li Acetylcholine Fluorescent sensor for in vivo acetylcholine...activity in vivo. Nat Methods. 2023 Nov 30. Yulong Li Dopamine Dopamine sensors GRAB_DA2 Next-generation...vivo. Nat Methods. 2020 Nov;17(11):1156-1166. Yulong Li Dopamine Ultrafast neuronal imaging of dopamine dynamics...2021 Nov 11. pii: 10.1038/s41587-021-01074-4. Yulong Li GABA GABA Sensor (iGABASnFR) (Constitutive or Cre-dependent...

  9. Retrograde AAV viral preps

    Type
    Collection
    ...fluorescent dopamine(DA) sensor GRAB_DA1h Dopamine sensor Li 123308 pAAV-hSyn-GRAB_NE1m Syn genetically-encoded...norepinephrine(NE) sensor GRAB_NE1m Norepinephrine sensor Li 123309 pAAV-hSyn-GRAB_NE1h Syn genetically-encoded...norepinephrine(NE) sensor GRAB_NE1h Norepinephrine sensor Li 123310 pAAV-hSyn-GRAB_NEmut Syn GRAB-NEmut control...sensor (does not respond to NE) Norepinephrine sensor Li 20297 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA...retrograde AAV based on the functional catagories listed below. Narrow down the items available within a...

  10. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...serotypes pAAV-hSyn-GRAB_OTmut AAV1 Biosensor Yulong Li New viral prep pAAV-CAG-NIR-GECO2G AAV1, AAV2,...

  13. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...Hires, S. A., Zhu, J. J., Cui, G., Lin, D., Du, J., & Li, Y. (2019). A Genetically Encoded Fluorescent Sensor...and resources References Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou, J., Wang, H., Zhang, ...

  14. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    ...gene is synthetic or natural? The answer to this lies in the codon usage biases of different organisms... in synthetic biology and DNA synthesis. They published their results in a paper in Nature Communications... Nielsen & Christopher A. Voigt also recently published a tool (trained on Addgene plasmid datasets) to...

  15. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    ... with the FP mNeptune684 (originally described by Li ZY, et al. PLoS One, 2016) and tested a series of...a recent plasmid deposit please sign up here.   Listen this episode of Hot Plasmids! Light controls the... dynamics Article contributed by Klaus Wanisch  Listen to the optogenetics podcast segment LOVTRAP is...localization Article contributed by Tyler Ford  Listen to the riboglow podcast segment Researchers from... story Article contributed by Caroline LaManna  Listen to the mCarmine podcast segment Scientists are ...activity Article contributed by Angela Kaczmarczyk  Listen to the base editing podcast segment The increasing...

  17. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ... F., Conatser, L. M., Lue, V. M., Dobbins, A. L., Li, C., Hirsch, M. L., & Samulski, R. J. (2020). Adeno-Associated...fmed.2023.1106085. PMID: 36817775. Wilmott, P., Lisowski, L., Alexander, I. E., & Logan, G. J. (2019). ...

  18. p53 Pathway

    Type
    Collection
    ...of tumors in early adulthood, a disorder known as Li-Fraumeni syndrome. Recently some impacts of p53 mutations...PubMed PMID: 19776742 . Germline TP53 mutations and Li-Fraumeni syndrome. Varley JM. Hum Mutat. 2003 Mar...pathway Background p53 Pathway Plasmids p53 Gene List References Background p53 is a transcription factor...transcriptional co-activators and modulate transcription. The list of p53 transcriptional targets is ever-expanding...available plasmids for the gene, or browse the gene list below. For multiple isoforms or subunits, individual...with the help of . Return to top p53 Pathway - Gene List Click on a name to find available plasmids for the... for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail help...
Showing: 41 - 60 of 771 results