We narrowed to 876 results for: NSI;
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TypeCollection...yeast, plants, or insect cells. Benefits include consistency between lots and the ability to optimize the ... NeuroMab collection - monoclonal antibodies extensively validated for neuroscience research applications...Recombinant mAb Collection . Once the plasmids are transiently transfected into mammalian tissue culture cells...
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mTOR Pathway
TypeCollection...production through glycolysis to fuel these energy-intensive processes. mTORC1 also blocks catabolic processes...substrate 1 (proline rich) PTEN Phosphatase and tensin homolog Rag RRAGA RRAGB RRAGC RRAGD Ras-related...known as PRR5; proline rich 5 PTEN Phosphatase and tensin homolog Rictor RPTOR independent companion of MTOR... -
Plant Plasmids and Resources
TypeCollection...transcription factors for Agrobacterium-mediated transient expression or plant transformation - Silin Zhong...CRISPR-mediated Plant Base Editors Save Time with Transient Plant Leaf Transformations RNA Interference in...interdisciplinary exchange, open technologies, and responsible innovation for improvement of sustainable agriculture... -
Viral Vectors
TypeCollection...high transduction efficiency and direct high but transient transgene expression. γ-Retrovirus Plasmids | ...8 Kb 80-130 Dividing/Non-dividing Yes Low AAV Transient or Stable* single stranded linear DNA ∼4.5 Kb ...Dividing/Non-dividing No* Very Low Adenovirus Transient double stranded linear DNA >8 Kb 105 Dividing/... -
Viral Production
TypeCollection...staining and the molecular weight and relative intensity of the viral capsid proteins are analyzed. The...preparation shows that the vast majority of the vectors consist of full particles (white arrowhead) relative to...targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease... -
Lentivirus Plasmids
TypeCollection...transgene expression. There are some safety considerations to evaluate when working with lentivirus: these... genome, it has the potential for insertional mutagensis. The 2nd and 3rd generation lentiviral systems...1a-driven GFP and shRNA under the control of a tet-responsive H1 promoter Trono 11651 pLVUT-tTR-KRAB 3rd Inducible... -
Neurodegeneration Research Collection
TypeCollection...speaking and swallowing. HD is caused by abnormal expansion of a repeating CAG triplet series within the huntingtin...or way to prevent progression of PD. Treatment consists mainly of managing symptoms through medication...Use a FRET-based method to monitor structural transitions in protein self-organization. Wan et al. Cell... -
Deisseroth INTRSECT Collection
TypeCollection...recognition sequences (e.g. Lox sites, FRT sites) inside of the introns (A,D). The addition of these recombinase...recombinases, viruses, and important controls to consider as part of experimental design. How-to guide (...Arber S, Lüthi A. 2016. Midbrain circuits for defensive behaviour. Nature 534(7606):206-12. PubMed (Link... -
CRISPR Pooled gRNA Libraries
TypeCollection...virtually any genetic locus. CRISPR pooled libraries consist of thousands of plasmids, each containing multiple...44,580 KSHV Tiling Library 180272 Knockout KSHV Glaunsinger 3rd NA 24,000 BLaER1 pgRNA Library 183825 Knockout... found to match all requested criteria. Please consider using fewer filtering options. Browse all libraries... -
Luciferase Plasmid Collection
TypeCollection...Reporter Constructs Luciferase is the enzyme responsible for the bioluminescence found in a diverse number... the emission of photons. The robust signal, sensitivity, wide dynamic range, and resistance to photobleaching...depending on the application. Firefly luciferase is considerably brighter than Renilla luciferase, but is more... -
Plasmids for Stem Cell Research
TypeCollection...codon-optimized mini-intronic plasmid for efficient, inexpensive, and xeno-free induction of pluripotency. Sci...861-72. Yamanaka Plasmid Human Non-integrating transient expression of human L-Myc, Lin28, Sox2, Klf4, ...35. Baldwin Fibroblasts iTSCs Lentiviral Mouse Extensive Nuclear Reprogramming Underlies Lineage Conversion... -
Fluorescent Protein Guide: Subcellular Localization
TypeCollection...3XGFP Microtubules ensconsin GFP William Bement 26742 EMTB-mCherry Microtubules ensconsin mCherry William...Return to top Cell Junctions Cell junctions are responsible for forming connections and adhesions between... -
Fluorescent Protein Guide
TypeCollection...Collection Browse the Michael Davidson comprehensive collection consisting of ORFs tagged with a variety of ... -
AAV Viral Preps
TypeCollection...vector preps are purified by density centrifugation. Addgene’s comprehensive high-quality quality control... -
Optogenetics Plasmid Collection
TypeCollection... a light sensitive protein from cryptochromes, phytochromes, or light-oxygen-voltage-sensing (LOV) proteins... -
CRISPR Plasmids - gRNAs
TypeCollection...was originally used. Here are a few questions to consider when choosing a gRNA: Does a given gRNA sequence...enzyme activity, PAM requirements, experimental considerations, and more, created and maintained by the Doudna... -
Zinc Finger Consortium: OPEN Reagents
TypeCollection...1000000013 ) Depositing Labs: Keith Joung This kit consists of plasmids and strains used to engineer ZFNs ...Oligomerized Pool ENgineering (OPEN) method. This kit consists of 5 individually packaged plasmids and 3 individually... -
Microbiology Resources
TypeCollection...plasmids, from networks and gene regulation to sensing and signaling, to perform your synbio experiment...system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid collection is organized... -
Worm Expression Resources
TypeCollection...transparent body of the C. elegans hermaphrodite consists of 959 somatic cells, which makes them attractive...elegans and related nematodes. WormBook - A comprehensive, open-access collection of original, peer-reviewed... -
TALEN Guide
TypeCollection...available as a kit from Addgene , custom arrays consisting of 12-31 repeats can be assembled and inserted... Zinc Finger Consortium with Dr. Voytas, has considerable experience in the DNA targeting field. A third...