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  1. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...ultracentrifugation of the viral stock, followed by resuspension of the collected particles in a smaller volume...white pellet. The pellet can then be rinsed and resuspended in cold sterile PBS.  Alternatively, to concentrate...

  3. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ..., homogeneous time resolved fluorescence, and suspension bead assay (Figure 2). After finding no cross-reactivity...scFVs to cognate aaRS antigens as measured by suspension bead assay. Image from  Preger et al., 2020. ...

  4. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ... wear comfortable shoes, as you will likely be spending a few hours on your feet. Pack water and snacks...you ever gone up to a poster where the presenter spends the entire time talking to the poster, as opposed...

  7. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...and contribute to my community. This past year, I spent some time thinking about how I could combine all...science communicators.  What can CRISPR do? I spent more time on this section of the presentation because...

  8. Tips for arabidopsis transformation

    Type
    Blog Post
    ...agrobacterial cultures are ready, spin them down and resuspend them in transformation media. If you are dipping...transformant. I like to spin down 100 mL of culture and resuspend in 25 mL of transformation medium per Agrobacterium...

  9. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...day, you can save the leftover bacteria-water suspension and use them to start cultures of your positive...up PCR reactions: The remaining bacteria-water suspension will serve as the template for your PCR reaction...

  10. Lab to Office Culture Shock

    Type
    Blog Post
    ...think about how much of that time you’re actually spending doing work. Yes, you may legitimately need to ...interact with...non-scientists! Maybe you’ve just spent 5-10 years learning to “speak science,” and virtually...

  12. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ... below “Tell a story”) and note how they build suspense. Notice the simplicity of their slides and how..., you just need to figure out how to make it suspenseful. 4. Start preparing your talk early - like today...

  13. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...Lysine-specific Demethylase 1 (LSD1) Tatjana Sauka-Spengler's lab has deposited pX330a dCas9-LSD1 for targeted...Artibani M, Taylor G, Wells D, Ahmed AA, Sauka-Spengler T (2017). Genome and epigenome engineering CRISPR...

  14. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ... your membranes with PBST to remove salt, then suspend a freshly harvested dose of cells (preferably in...enjoys pondering how phages get through the day, spending time with her horse, and wondering what her cat...

  16. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...depending on the revisions and the amount of time spent shopping a manuscript. Manuscript writing can take...fit into a future manuscript? You could easily spend a day or a week planning an experiment, even a relatively...

  19. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ...was so moved by our deck the lab contest that she spent a year hoarding used tip boxes for a lab supply ...
Showing: 41 - 60 of 180 results