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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 53 results for: Z-ISO

Showing: 41 - 53 of 53 results

  1. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ..., Gola, A., Thakur, N., Shih, R., Yao, L., Yaniv, Z. R., Beuschel, R. T., Kabat, J., Croteau, J., Davis...primary antibodies are all of different species/isotypes and that each secondary has a different color ...Lab, using recombinant antibodies with different isotypes. (A) Neocortex labeled with anti-pan-Nav (magenta...using primary antibodies from different species or isotypes, so that the secondary antibodies don’t cross-...whichever combination of host and target species, isotype specificity, and dye meets your needs and budget...with a primary antibody of a different species/isotype; when you add the secondary antibody, it should...primary antibody that has the same species and isotype as the primary antibody in your experiment). Confirm...

  2. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...j.neures.2014.09.002 Zingg, B., Chou, X. lin, Zhang, Z. gang, Mesik, L., Liang, F., Tao, H. W., & Zhang, ...designing and testing your AAV.  Figure 2: Comparison of AAV serotypes 1, 2, 5, 6, 8, and 9 in two ...Taschenberger et al., 2017).     Figure 3: Comparison of two astrocyte selective promoters demonstrating...vector-mediated transduction of the corticospinal tract: Comparison of four promoters. Gene Therapy, 28(1–2), 56–...

  3. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ..., Bhattacharya, K., Pyrgiotakis, G., Zhang, Z., Purington, E., Gramlich, W., Grondin, Y., Rogers, R., ...The most-used fluorescent dye is fluorescein isothiocyanate (FITC), with excitation/emission peak at 495...For instance, the fluorescence of fluorescein isothiocyanate (FITC) can be stably combined with nanoparticles... Darzi, M., Tarahomi, M., & Ghods, R. (n.d.). Comparison of the Photobleaching and Photostability Traits...Traits of Alexa Fluor 568-and Fluorescein Isothiocyanate-conjugated Antibody. Salari, M., Bitounis, D., ...

  4. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...Baguley, S. W., Richens, J. H., Wissner, R. F., Xi, Z., Allgeyer, E. S., Zhong, S., Thompson, A. D., Lowe...STED) nanoscopy. Scale bar, 2 μm. Inset shows comparison with standard confocal microscopy. Image adapted...159 aa) are smaller than HaloTag (295 aa) – for comparison, common fluorescent proteins are typically 230...Strategies Matter for Super-Resolution Microscopy: A Comparison between HaloTags and SNAP-tags. Cell Chemical...

  5. CRISPR Guide

    Type
    Guide
    ... PMID: 23287718 Cui, Z., Tian, R., Huang, Z., Jin, Z., Li, L., Liu, J., Huang, Z., Xie, H., Liu, D., Mo... Liu, Y., Gu, Z., Dickerson, K. E., Xie, S., Hon, G. C., Xuan, Z., Zhang, M. Q., Shao, Z., & Xu, J. (2017...Topkar, V. V., Nguyen, N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J. J., Aryee... 35236982 Bubeck, F., Hoffmann, M. D., Harteveld, Z., Aschenbrenner, S., Bietz, A., Waldhauer, M. C., ...H., Zhou, R., Lang, B., Meng, B., Weng, H., & Hu, Z. (2022). FrCas9 is a CRISPR/Cas9 system with high ... 24463574 Gao, S., Wang, Y., Qi, T., Wei, J., Hu, Z., Liu, J., Sun, S., Liu, H., & Wang, Y. (2023). Genome...Sun, N., Zeina, C. M., Gao, X., Rees, H. A., Lin, Z., & Liu, D. R. (2018). Evolved Cas9 variants with ...

  6. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...tRNA-gRNA (PTG) constructs. Eukaryotic RNases P and Z recognize the tRNA sequences, cleave them, and release...supply it with another plasmid.   Figure 3: Comparison of Multiplex Strategies including Standard PolIII-gRNA...

  7. Deep Dive: qPCR

    Type
    Blog Post
    ... An indicator, not a diagnosis. IDT, 2014. Dwight Z; Palais R.; Wittwer CT. uMELT: prediction of high-...multiplex (Thellwell, 2000). See Table 1 for a comparison of features of several commonly used chemistries... See Table 2, (adapted from Wong, 2018) for a comparison of different analysis methods.   Method ...defined log-linear phase   Table 2: A comparison of different qPCR analysis methods. Table adapted...

  9. Adenovirus Guide

    Type
    Guide
    ...0334-z (Link opens in a new window) PMID: 31659156 (Link opens in a new window) Luo, J., Deng, Z. L., ... Yu, X., Farina, E. M., Yan, S., Zhao, C., Zheng, Z., Shu, Y., Wu, X., Lei, J., Li, Y., Zhang, W., Yang... nucleus, normally as double-stranded circular episomes. Due to this, and to the strong host’s immune ...genome and instead are maintained in the cell as episomes. This highly reduces the potential for insertional...nonenveloped viruses from the Adenoviridae family. Episome Genetic element inside the cell that remains as...

  10. Optogenetics Guide

    Type
    Guide
    ...Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, et al. 2014. Independent optical excitation of distinct...These tools have been instrumental in neurological disorder research, helping scientists to better understand...Halorhodopsins are light-gated inward chloride pumps isolated from halobacteria. Wild-type halorhodopsin, known...

  11. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...) PMID: 27720486 (Link opens in a new window) Wu, Z., Asokan, A., & Samulski, R. J. (2006). Adeno-associated...typically processed into a double-stranded circular episome which can concatemerize, producing high molecular...majority of rAAV DNA is thought to be maintained in episomes or concatemers. This avoids the effects local ... and the expression of neighboring genes. rAAV episomes can develop chromatin-like organization and persist...period of years without damaging the host cell. Episomal stability allows for long-term transgene expression...in the field of gene therapy to treat genetic disorders. rAAV vectors are favored over other viral delivery...trans-splicing or overlapping vectors: a quantitative comparison . Molecular Therapy, 4 (4), 383–391. https://...

  12. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...T7 ALS Andreas Martin 226783 pFB-EPCP1 LRRK2 His, Z, TEV polH Parkinson's Sebastian Mathea 227004 pAAV-CMVenh...Parkinson's Wade Harper 228879 pFB-EPCP5 LRRK2 His, Z, TEV polH Parkinson's Sebastian Mathea 228917 sg_Atm_i3...SLC1A3 PGK Episodic ataxia Walker Jackson 129410 px335 Slc1a3 nickase 1 SLC1A3 HA U6, Cbh Episodic ataxia ... KCNA1 U6 Episodic ataxia Xiaolong Wang 159788 pGL3-U6-sgRNA_KCNA1-mcherry KCNA1 U6 Episodic ataxia Xiaolong...Flag CMV, T7 ALS Alison Twelvetrees 200790 CLIPf-KIF5A KIF5A CLIPf CMV, T7 ALS Alison Twelvetrees 200791...Parkinson's Ted Dawson 17706 GLASTp-DsRed2 SLC1A3 DsRed2 Episodic ataxia Nicholas Gaiano 17797 pM-ErbB4CTF ERBB4...GST T7 ALS Aaron Gitler 29638 pGEX-MK1 KCNA1 tac Episodic ataxia Bruce Tempel 30137 pCAX APP 695 APP CAG...

  13. Immunology Research Plasmids and Resources

    Type
    Collection
    ...Dunn P, Thomas CG, Smith B, Schaefer H, Chen J, Hu Z, Zalocusky KA, Shankar RD, Shen-Orr SS, Thomson E,...disulfide isomerase family A, member 2 PDA2, PDI, PDIP, PDIR PDIA3 protein disulfide isomerase family A...gamma isoform CALNA3 PPP3R1 protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform CALNB1...phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform CALN, CALNA, CALNA1, CCN1, CNA1, PPP2B PPP3CB protein...phosphatase 3 (formerly 2B), catalytic subunit, beta isoform CALNA2, CALNB PPP3CC protein phosphatase 3 (formerly...
Showing: 41 - 53 of 53 results