...plasmids used in this publication at Addgene. Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA...other chronic diseases, including dementia, gut disorders, nerve damage, and cardiac disease. This increased... could be rescued by CRISPR. CD4+ T-cells were isolated from healthy individuals, expanded, and infected...CRISPR reduced the HIV-1 copy number by over 50% in isolated CD4+ T-cells from two HIV-1 infected patients....

..., Gola, A., Thakur, N., Shih, R., Yao, L., Yaniv, Z. R., Beuschel, R. T., Kabat, J., Croteau, J., Davis...primary antibodies are all of different species/isotypes and that each secondary has a different color ...Lab, using recombinant antibodies with different isotypes. (A) Neocortex labeled with anti-pan-Nav (magenta...using primary antibodies from different species or isotypes, so that the secondary antibodies don’t cross-...whichever combination of host and target species, isotype specificity, and dye meets your needs and budget...with a primary antibody of a different species/isotype; when you add the secondary antibody, it should...primary antibody that has the same species and isotype as the primary antibody in your experiment). Confirm...

...j.neures.2014.09.002 Zingg, B., Chou, X. lin, Zhang, Z. gang, Mesik, L., Liang, F., Tao, H. W., & Zhang, ...designing and testing your AAV.  Figure 2: Comparison of AAV serotypes 1, 2, 5, 6, 8, and 9 in two ...Taschenberger et al., 2017).     Figure 3: Comparison of two astrocyte selective promoters demonstrating...vector-mediated transduction of the corticospinal tract: Comparison of four promoters. Gene Therapy, 28(1–2), 56–...

..., Bhattacharya, K., Pyrgiotakis, G., Zhang, Z., Purington, E., Gramlich, W., Grondin, Y., Rogers, R., ...The most-used fluorescent dye is fluorescein isothiocyanate (FITC), with excitation/emission peak at 495...For instance, the fluorescence of fluorescein isothiocyanate (FITC) can be stably combined with nanoparticles... Darzi, M., Tarahomi, M., & Ghods, R. (n.d.). Comparison of the Photobleaching and Photostability Traits...Traits of Alexa Fluor 568-and Fluorescein Isothiocyanate-conjugated Antibody. Salari, M., Bitounis, D., ...

...Baguley, S. W., Richens, J. H., Wissner, R. F., Xi, Z., Allgeyer, E. S., Zhong, S., Thompson, A. D., Lowe...STED) nanoscopy. Scale bar, 2 μm. Inset shows comparison with standard confocal microscopy. Image adapted...159 aa) are smaller than HaloTag (295 aa) – for comparison, common fluorescent proteins are typically 230...Strategies Matter for Super-Resolution Microscopy: A Comparison between HaloTags and SNAP-tags. Cell Chemical...

...tRNA-gRNA (PTG) constructs. Eukaryotic RNases P and Z recognize the tRNA sequences, cleave them, and release...supply it with another plasmid.   Figure 3: Comparison of Multiplex Strategies including Standard PolIII-gRNA...

... An indicator, not a diagnosis. IDT, 2014. Dwight Z; Palais R.; Wittwer CT. uMELT: prediction of high-...multiplex (Thellwell, 2000). See Table 1 for a comparison of features of several commonly used chemistries... See Table 2, (adapted from Wong, 2018) for a comparison of different analysis methods.   Method ...defined log-linear phase   Table 2: A comparison of different qPCR analysis methods. Table adapted...

... PMID: 23287718 Cui, Z., Tian, R., Huang, Z., Jin, Z., Li, L., Liu, J., Huang, Z., Xie, H., Liu, D., Mo... Liu, Y., Gu, Z., Dickerson, K. E., Xie, S., Hon, G. C., Xuan, Z., Zhang, M. Q., Shao, Z., & Xu, J. (2017...Topkar, V. V., Nguyen, N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J. J., Aryee... 35236982 Bubeck, F., Hoffmann, M. D., Harteveld, Z., Aschenbrenner, S., Bietz, A., Waldhauer, M. C., ...H., Zhou, R., Lang, B., Meng, B., Weng, H., & Hu, Z. (2022). FrCas9 is a CRISPR/Cas9 system with high ... 24463574 Gao, S., Wang, Y., Qi, T., Wei, J., Hu, Z., Liu, J., Sun, S., Liu, H., & Wang, Y. (2023). Genome...Sun, N., Zeina, C. M., Gao, X., Rees, H. A., Lin, Z., & Liu, D. R. (2018). Evolved Cas9 variants with ...

...future! References Bubeck F, Hoffmann MD, Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer MC, Börner K,...of transferrin receptor CD71 in HeLa cells.   Comparison of photoactivatable CRISPR strategies Publication...

...0334-z (Link opens in a new window) PMID: 31659156 (Link opens in a new window) Luo, J., Deng, Z. L., ... Yu, X., Farina, E. M., Yan, S., Zhao, C., Zheng, Z., Shu, Y., Wu, X., Lei, J., Li, Y., Zhang, W., Yang... nucleus, normally as double-stranded circular episomes. Due to this, and to the strong host’s immune ...genome and instead are maintained in the cell as episomes. This highly reduces the potential for insertional...nonenveloped viruses from the Adenoviridae family. Episome Genetic element inside the cell that remains as...

...Cho YK, Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, et al. 2014. Independent optical excitation of distinct...These tools have been instrumental in neurological disorder research, helping scientists to better understand...Halorhodopsins are light-gated inward chloride pumps isolated from halobacteria. Wild-type halorhodopsin, known...

...) PMID: 27720486 (Link opens in a new window) Wu, Z., Asokan, A., & Samulski, R. J. (2006). Adeno-associated...typically processed into a double-stranded circular episome which can concatemerize, producing high molecular...majority of rAAV DNA is thought to be maintained in episomes or concatemers. This avoids the effects local ... and the expression of neighboring genes. rAAV episomes can develop chromatin-like organization and persist...period of years without damaging the host cell. Episomal stability allows for long-term transgene expression...in the field of gene therapy to treat genetic disorders. rAAV vectors are favored over other viral delivery...trans-splicing or overlapping vectors: a quantitative comparison . Molecular Therapy, 4 (4), 383–391. https://...

...Dunn P, Thomas CG, Smith B, Schaefer H, Chen J, Hu Z, Zalocusky KA, Shankar RD, Shen-Orr SS, Thomson E,...disulfide isomerase family A, member 2 PDA2, PDI, PDIP, PDIR PDIA3 protein disulfide isomerase family A...gamma isoform CALNA3 PPP3R1 protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform CALNB1...phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform CALN, CALNA, CALNA1, CCN1, CNA1, PPP2B PPP3CB protein...phosphatase 3 (formerly 2B), catalytic subunit, beta isoform CALNA2, CALNB PPP3CC protein phosphatase 3 (formerly...