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...CRISPR? Experts cover CRISPR topics on Addgene's blog. Addgene's Viral Service : Request ready-to-use viral...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR...technologies are continously developing and expanding. Addgene works with leading scientists in the field to ensure...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety...zebrafish, C. elegans, parasites, and Xenopus. Addgene CRISPR Resources CRISPR Guide : Essential background...

... TALENs TALEN Guide Addgene's Guide to TALEN Technologies You may also like....Zinc Finger Consortium Originally published in Addgene's December 2011 Newsletter Overview of TALEN Technology...these plasmids, which are available as a kit from Addgene , custom arrays consisting of 12-31 repeats can...and has already become the best-selling kit in Addgene’s history. Dr. Keith Joung’s lab at Massachusetts...Hospital also recently released a TALEN kit through Addgene. This kit, first described in Nature Biotechnology...at the Broad Institute, will be available from Addgene before the end of the year. Dr. Zhang ‘s lab published...could become a key tool in your plasmid toolbox. Addgene has rapidly become an important resource for this...

...assistance at help@addgene.org . Don’t See What You’re Looking For? At this time, Addgene has made a limited...Grade DNA Service Details Plasmids Available FAQ Addgene is offering Cloning Grade DNA (cgDNA) for over ...start cloning upon arrival of their plasmid from Addgene – reducing the time to experiments by removing ...resources or scientific support? Please see our Addgene Protocols for resources you may find helpful. Our... Please note that this does not guarantee that Addgene will make cloning grade DNA preps of this plasmid...

...benefits of depositing plasmids with Addgene or email deposit@addgene.org to get started. Genes KLF gene pages...Browse Addgene's collection of KLF plasmids and learn more about the scientists who have created and ...to the establishment of this shared resource on Addgene. If you have created plasmids containing KLF, we... KLF1 KLF2 KLF4 KLF5 KLF6 KLF Plasmids Browse Addgene's collection of KLF plasmids. ID Plasmid Description...

...Ready-to-use AAV available from Addgene's viral service. Engineered systemic constructs from Viviana ...As part of our Viral Vector Packaging Service , Addgene is distributing ready-to-use viral preparations...depth descriptions of each serotype, check out Addgene's blog on Systemic Capsids . To review performance...data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER Center’s repository...produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005) . Note on PHP.eB tropism and specific ... produced with the pUCmini-iCAP-PHP.S plasmid (Addgene #103006) . Browse Available PHP.S AAV ID Name Promoter...produced with the pUCmini-iCAP-PHP.V1 plasmid (Addgene #127847) . Browse Available PHP.V1 AAV ID Name ...

...Industry Additional Addgene Resources Blog: Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids... viral proteins in several expression systems. Addgene partnered with Ginkgo to make these plasmids available...expressed or functionally validated by Ginkgo or Addgene. Plasmids Use the search bar or sort buttons to...

... to find the plasmids that are available from Addgene's depositing scientists.... Fluorescent Proteins: Plasmids and Resources Addgene's repository contains thousands of fluorescent protein...organs and whole animals. Additional Resources at Addgene Optogenetics Guide | Optogenetics Plasmids Use ...signaling pathways, and more. Learn More and Explore Addgene Blog: Fluorescent Proteins Fluorescent Protein ...

...about synthetic biology resources available at Addgene. Browse featured collections or depositing labs...engineering of biological systems for novel functions. Addgene is working with leading synthetic biologists around...facilitate the sharing of parts and information. Addgene can help you to share your synthetic biology plasmids...signaling and environmental sensing. Strains Browse Addgene's collection of engineered bacterial strains, including...Resource Allocator and Orthogonal Switches Other Addgene Tools and Resources CRISPR Fluorescent Proteins...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm CRISPR...highlight some of the plasmids and tools available at Addgene for use in worms. Browse Plasmids Plasmid Tools...of plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description Gene...Biology Synthetic biology constructs - Browse Addgene's collection of synthetic biology plasmids for use... in C. elegans . Return to top Other Resources Addgene Blog Posts An “elegans” Approach to Better CRISPR...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...Browse the Open Enzyme collection of plasmids at Addgene for generating enzymes for molecular biology lab...Transcriptases RNA Polymerases Other Enzymes DNA Ligases Addgene ID Plasmid Gene/insert 165503 pOpen-HSlig4 DNA ...pOpen-HSlig3 DNA Ligase III Return to top DNA Polymerases Addgene ID Plasmid Gene/insert 165500 pOpen-9N7polA 9 degrees...Polymerase Return to top Restriction Endonucleases Addgene ID Plasmid Gene/insert 165504 pOpen-EcoRIR EcoRI...pOpen-NcoIR NcoI Return to top Reverse Transcriptases Addgene ID Plasmid Gene/insert 165546 pOpen-MMLV_RT (mut...Reverse Transcriptase Return to top RNA Polymerases Addgene ID Plasmid Gene/insert 165505 pOpen-T7gene1 T7 ... T3 RNA Polymerase Return to top Other Enzymes Addgene ID Plasmid Gene/insert 165509 pOpen-TEV protease...

...Browse Addgene's COVID-19 collection of SARS-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids...protein and you will find examples of each in Addgene's collection: HIV-based lentiviral particles Murine...particles Vesicular stomatitis virus particles See Addgene's Viral Plasmids and Resources page for more information...virus protocol for coronaviruses (DOCX, 184 KB) Addgene Blog Posts Viral Vectors 101: Pseudotyping Antibody...Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants Addgene COVID-19 Resources and Collections COVID-19 and...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...using shuttle vectors pICPIS-EF1 (Addgene #73355) and pICPIS-CB (Addgene #73356) . pICPIS-EF1 contains the...lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors Within this set of...actin promoter with CMV enhancer. pAdx-CMV-LacZ (Addgene #73345) is uniquely suited to simplify the cloning...chicken-beta actin CMV enhancer promoter SV40 polyA Addgene Resources For more information on lentiviral and...Lentiviral Plasmids and Adenoviral Plasmids collections Addgene Blog: Your Lentiviral Plasmid FAQs Biosafety Resource...

... Addgene Homepage Plasmid Collections Addgene Plasmid Collections Addgene's repository contains...Browse Addgene’s curated collections of plasmids, kits, and pooled libraries. Find tools for CRISPR, ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...between the Cell Migration Consortium (CMC) and Addgene, which highlights CMC depositing labs and plasmids...collaboration between the Cell Migration Consortium and Addgene. Member laboratories of the Cell Migration Consortium...plasmids useful for cell migration research at Addgene. CMC investigators who have deposited plasmids ...

...FLEx switch and what you can do with it, read Addgene's blog on FLEx Vectors . Flp-IN: A single FRT site...Description Vector Type Promoter PI Additional Addgene resources Explore our full catalog for plasmids... Packaged on Request service for more options. Addgene blog Plasmids 101: Cre-lox Cre-ating New Methods...Description Vector Type Promoter PI Additional Addgene resources Search our full catalog for plasmids ... Packaged on Request service for more options. Addgene blog Advanced Uses of Cre-lox and Flp-FRT — A Neuroscientist... Plasmid Description Vector Type PI Additional Addgene resources Use the target site sequence for your...

...Addgene's COVID-19 collection of molecular tools and resources available for Coronavirus research....understanding of COVID-19 and related coronaviruses. Addgene assisted this effort by providing a platform for... ID Plasmid Description Industry PI Additional Addgene resources SARS-CoV-2 Viral Pseudotyping Collection...plasmids. Ginkgo Bioworks Plasmid Collection – Addgene partnered with Ginkgo Bioworks to distribute SARS-CoV...protein constructs for making VSV pseudo virus. Addgene blog Scientists Map the SARS-CoV-2-Human Interaction... ID Plasmid Description Industry PI Additional Addgene resources ACE2 plasmids - Host cell receptor mediating...plasmids And more! ID Plasmid Description Industry PI Addgene blog Finding nucleic acids with SHERLOCK and DETECTR...

...pAdEasy-1 (Addgene #16400) plasmid Vogelstein Return to top Other Resources Resources on addgene.org Adenoviral...bacterial strains for adenoviral vector production in Addgene's repository. Viral...Vogelstein 18703 pEZYshuttle Modified pShuttle-CMV (Addgene #16403) plasmid with Gateway cassette Zhang 50958...Vectors 101 eBook Viral Vectors 101 YouTube Videos Addgene's Biosafety Resources Protocols General Transfection...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...plasmids that have been used in these efforts. Addgene's parasite CRISPR plasmid collection also includes...protein-coding genes. To find emtpy gRNA plasmids, visit Addgene's Empty gRNA Expression Vectors page and search ... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...indicators, and rabies targeting genes. Recombinases Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral...pAAV-CaMKIIa-Flpo None Yes Single recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral...Vcre No Dual recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral...AND NOT Cre Yes Dual recombinase-dependent: GECI Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral... Dual recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral... Dual recombinase-dependent: Inhibitory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral...NOT Cre Yes Dual recombinase-dependent: DREADDs Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral...

...which Cas has been integrated into the genome. Addgene has a large collection of empty gRNA vectors. Explore...Multiplex gRNA Kits Many CRISPR plasmid kits at Addgene contain multiplex-compatible gRNA plasmids, including...CRISPR/Cas Toolkit for Plants CRISPR Resources Addgene has a large selection of other CRISPR plasmids ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...