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We narrowed to 210 results for: cycs

Showing: 41 - 60 of 210 results

  1. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ... of XFPs in the original method, we used the Tetracycline trans-activator system to boost the expression...expression levels, and we named the system Tetbow (Tetracycline trans-activator Brainbow) (Sakaguchi et al.,...

  4. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...reporter mRNAs. Using translation inhibitors cycloheximide and puromycin, Halstead et al. verified that...effects of 5’ UTR variants in translation of cell cycle protein Emi1, showing that ~80% of transcripts with...

  5. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...for infection of quiescent cells or infrequently cycling cells but is fine for experiments with immortalized...integration to express its cargo, in fact its viral life cycle does not include genomic integration. While the ...

  6. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ...you’re working with them, but avoid freeze-thaw cycles, as they’ll degrade your antibody.  At some point...been left out, gone through a freeze-thaw-freeze cycle before, or otherwise been subject to mishandling...

  10. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...some viral preps are very sensitive to freeze thaw cycles; the titer measured with a freshly collected lentiviral...aliquot that has been subjected to a freeze/thaw cycle for titering as this will better represent future...

  11. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ... to increase yield. The collection process is a cycle of collecting the media and adding fresh media every...Baculovirus replicates inside insect cells, and its lifecycle ends in cell lysis, releasing the virus to the...

  12. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ... target cells and can be altered by freeze/thaw cycles (Lock et al., 2010). It is recommended that you...are quite stable and will withstand freeze/thaw cycles and dehydration (Howard & Harvey, 2017). You can...

  15. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...few things you can try: Increase the number of cycles. After additional rounds of amplification, your...positive and negative droplets. No more than 50 cycles is recommended. Decrease your ramp rate. A rate...

  16. MXS Chaining

    Type
    Blog Post
    ..., one can generate constructs to visualize cell cycle dynamics, titrate inducible transgene expression...later construct (i.e.,functional cassettes are recyclable) Can only work if the restriction sites used ...

  17. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...is tightly coupled to the bacterial host's cell cycle, maintaining a stable concentration of plasmid. ...copy number of pSC101 through a negative feedback cycle. As with DnaA, high concentrations of RepA negatively...

  18. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...generally aliquoted to reduce the number of freeze-thaw cycles needed when using the virus. A small aliquot is...lentivirus. Since viruses are sensitive to freeze/thaw cycles, we include a 1.3 mL aliquot with mostlibrary requests...

  19. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...splice acceptor. TRE General expression mRNA Tetracycline response element promoter Inducible with Tetracyline...promoter with low basal activity and several tetracycline operators. Transcription can be turned on or...

  20. Five Popular Model Organisms

    Type
    Blog Post
    ...grow and maintain in the lab, have a short life cycle of 8 to 14 days, and produce a large number of offspring...small, transparent worms that have a rapid life cycle and large brood sizes. The majority of C. elegans...
Showing: 41 - 60 of 210 results