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Showing: 41 - 60 of 429 results

  1. AAV Viral Preps

    Type
    Collection
    ...DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre...

  2. Optogenetics Plasmid Collection

    Type
    Collection
    ... cells. Other microbial opsins, such as the Halorhodopsins, Archaerhodopsins and Leptosphaeria rhodopsins...

  3. Mammalian RNAi Tools

    Type
    Collection
    ...PubMed (Link opens in a new window) . Dana, H., Chalbatani, G. M., Mahmoodzadeh, H., Karimloo, R., Rezaiean...

  4. Microbiology Resources

    Type
    Collection
    ...by Microbe System Plasmids for Archaea Species Haloferax sp. Methanococcus maripaludis Methanothermobacter...

  5. Arf GTPase Family

    Type
    Collection
    ...GTPases and their GEFs and GAPs: concepts and challenges. Molecular Biology of the Cell , 30 (11), 1249...

  6. p53 Pathway

    Type
    Collection
    ...early ageing-associated phenotypes. Tyner SD, Venkatachalam S, Choi J, Jones S, Ghebranious N, Igelmann ...

  11. Optogenetics AAV Preps

    Type
    Collection
    ...AAV collection, including channelrhodopsins, halorhodopsins, and more....

  12. Caltech Systemic Capsids

    Type
    Collection
    ...Link opens in a new window) and was adapted from Challis et al. (2022). Text version of the AAV decision...

  13. Bacterial Expression Systems

    Type
    Collection
    ...purified protein. Protein purification can be challenging, but once you get it right, you can learn many...

  14. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Chari 3rd 5 30,868 35,517 Focused Ras Synthetic Lethal Human CRISPR Knockout Library 92352 Knockout Human...

  15. Plasmids for Stem Cell Research

    Type
    Collection
    ...iPSCs, much like their ES cell cousins, have the hallmarks of pluripotent cells--they are capable of differentiating...

  16. Optogenetics Guide

    Type
    Guide
    ...plasmids . Halorhodopsins Halorhodopsins are light-gated inward chloride pumps isolated from halobacteria. Wild-type...Jaws Browse Halorhodopsin plasmids . Archaerhodopsins Archaerhodopsin-3 (Arch) from Halorubrum sodomense...variant 517 Halorhodopsins Jaws Red-shifted, light-driven inward chloride pump from Haloarcula (Halobacterium...when one half of a given protein, such as the Cre recombinase, is fused to Cry2 and the other half to CIB1...Wild-type halorhodopsin, known as NpHR (from Natronomonas pharaoni ), causes hyperpolarization (inhibition...enhancements such as: Human codon optimization Example: Halo Increased photocurrent amplitude Examples: eNpHR...strain Shark), used to silence neuronal activity 632 Halo/NpHR Light-driven inward chloride pump from Natronomonas...

  18. CRISPR Guide

    Type
    Guide
    ...less widely used in bacteria due to technical challenges, bacterial CRISPR libraries have been developed... S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., Cradick, T. J., Marraffini, L. A., Bao, G....., Gootenberg, J. S., Kriz, A. J., Zetsche, B., Shalem, O., Wu, X., Makarova, K. S., Koonin, E. V., Sharp...790. PMID: 38216671 Klompe, S. E., Vo, P. L. H., Halpin-Healy, T. S., & Sternberg, S. H. (2019). Transposon-encoded...Communications . 9 (11), e1007749. PMID: 30575746 Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott...cells. Science . 343 (6166), 84–87. PMID: 24336571 Shalem, O., Sanjana, N. E., & Zhang, F. (2015). High-throughput...Meadows, S. K., Roberts, B. S., Mackiewicz, M., Mendenhall, E. M., & Myers, R. M. (2015). CETCh-seq: CRISPR...

  19. Immunology Research Plasmids and Resources

    Type
    Collection
    ...PRLTS PDYN prodynorphin MGC26418, PENKB PENK proenkephalin - PF4 platelet factor 4 CXCL4, MGC138298, SCYB4... Cell Mobility Using Fluorescent Proteins The Challenges of Cell Culture External Resouces Immport : Bhattacharya...

  20. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...known as HaloTag is actually HaloTag7, and recent specialized variants run up to HaloTag11. HaloTag is now... (Gallagher et al., 2009). HaloTag: Scientists at Promega developed HaloTag in 2008 (Los et al., 2008)...2008), based on a bacterial haloalkane dehalogenase that binds a synthetic chloralkane (CA) ligand. The current...159 Trimpethoprim  (TMP-tag) Few Easy HaloTag 295 Chloralkane (CA) Many Easy CLIP-tag...proteins (often up to 10-fold more). So, labeling a HaloTag or SNAP-tag construct can help you better detect...) Labeling scheme. b) COS-7 cell ER labeled with Halo-Sec61β with SiR-CA (magenta) and outer mitochondrial...protein of interest. For example, the Cohen lab used HaloTag for their “ticker tape” method for intracellular...
Showing: 41 - 60 of 429 results