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We narrowed to 413 results for: his

Showing: 41 - 60 of 413 results

  1. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...which can be carried out using Addgene plasmids. His-tag labeling reagents You may already have a FRET...used for protein purification: the NTA binds to the His tag on your protein and the coupled Cy fluorophore... Tc: CCPGCC 50 40-72 Intact Cells Cy3NTAc Poly His  44 35-63 Plasma membrane protein, permeabilized ... Tc: CCPGCC 48 38-70 Intact Cells Cy3NTAc Poly His  63 50-90 Plasma membrane protein, permeabilized ... Tc: CCPGCC 54 42-78 Intact Cells Cy3NTAc Poly His  65 52-94 Plasma membrane protein, permeabilized ...efficiencies between 80% and 10%. C Cy3NTA and Cy5NTA bind His tags similarly but with differing R0 values, thereby...department at Brigham and Women’s Hospital in Boston, MA. His interests include developing novel fluorescent tools...

  2. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    ...cooks and cleans with his family, “sometimes listening to audiobooks.” Church and his family spend family...Ellis highlights the importance of acknowledging his teams successes. “When PhD students graduate there...

  3. TALEN Guide

    Type
    Collection
    ...21822235 . See the Q&A with Dr. Adam Bogdanove and his thoughts on the TALe technology here ....already become the best-selling kit in Addgene’s history. Dr. Keith Joung’s lab at Massachusetts General...

  4. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...instead started a company around his own research. He was clear on his science, and found a solution. However...manageable laboratory environments. His business skills enhance his passion for science. Follow him on ...to provide the resources required for him to meet his goals. Therefore, he turned from academia altogether...

  5. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ... Children’s Hospital and Harvard Medical School. His lab studies the molecular genetics of blood cell ...cell development and disease. Learn more at his webpage, bauerlab.org.     Matthew Canver is an MD/PhD student...laboratories of Stuart Orkin and Daniel Bauer. He received his BSE in Bioengineering from the University of Pennsylvania...these may require restriction fragment length polymorphism analysis, allele-specific PCR, T7EN1 cleavage...

  6. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...problems resulting from past practices and mores.  His list is dire:  The current system is unsustainable... be in academia to make change.  He talked about his own unique way of funding basic research at NEB and... of the Novartis industry postdoctoral program.  His strongest comments were about the astounding lack...

  8. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    ... Embracing Serendipity Nicolas Mathis, who shared information on his open software that predicts efficiencies.... Illuminating Choices Paul Heisig, who shared his flow cytometry expertise in three posts. An Introduction...

  9. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...given over the course of the day. His talk, which focused on the history of genome engineering from ZFNs...mentioned specifically in Dr. Zhang's introduction. His willingness to share reagents so freely with the ...analog versions of each, and Tim Lu even compared his "memory" gene codes to a Turing machine (also discussed... of the book on my way to the meeting, and its history of code making and code breaking is wonderfully...

  10. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...about his own biases--many of which will be shared by fellow scientists--but that doesn’t make his introduction...case studies are “old,” they lead Zimmer to one of his most critical observations: “Today the most successful...if Zimmer was able to rewrite the last chapter of his book today--the day you are reading this review--...

  11. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ... successful applicants and get chosen to work in his or her lab of choice? Is the group micromanaged or...to the lab to see how they fit in with the team. His advice is “if you’re within a year from the end of...the lab for a full day, gives a 1 hour talk about his or her interests, and spends one-on-one time with... hires are simpler because of their documented history in science. Potential graduate students typically...

  14. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...Awards and we couldn’t wait to congratulate him on his successes. We've been lucky enough to meet many depositing...Addgene depositing scientist – stop by our booth to say hi! Zhang was awarded one of this year’s SfN Young Investigator...

  15. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ...science lab.” Introducing GreenGate As Lohmann and his colleagues describe in the PLoS ONE paper that introduced... it no longer works, just trash it.” Lohmann and his colleagues including Joachim Forner now expect and...

  16. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ... Dr. Deisseroth has also generously made many of his optogenetic constructs available through the vector... at the University of North Carolina) that offer his constructs. Importantly, control viruses (not expressing...guest blogger Derek Simon! Derek P. Simon received his PhD in Cellular and Molecular Biology from the University...Mary Jeanne Kreek at the Rockefeller University.  His interests include the role of learning and memory...is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org or follow him on Twitter...and for good reason. For the first time in the history of neuroscience research we have a technology that...

  18. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...journal Science Signaling in December 2014, he and his colleagues at Duke University, in collaboration with...Applications of the cancer pathways ORFs kit Wood and his colleagues developed the kit for use in screening...

  20. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...presented with a physical model of his large ribosomal subunit, he kept sliding his palm across the surface of... about Jacob’s initial reaction to this model on his blog. Or, for a description of how effective this...Addgene.org Visit Our CRISPR Guide Page CRISPR/Cas9 History Browse All CRISPR Plasmids and Resources ...
Showing: 41 - 60 of 413 results