Special Delivery: Fluorophore Targeting for FRET Studies
Type
Blog Post
...which can be carried out using Addgene plasmids.
His-tag labeling reagents
You may already have a FRET...used for protein purification: the NTA binds to the His tag on your protein and the coupled Cy fluorophore...
Tc: CCPGCC
50
40-72
Intact Cells
Cy3NTAc
Poly His
44
35-63
Plasma membrane protein, permeabilized ...
Tc: CCPGCC
48
38-70
Intact Cells
Cy3NTAc
Poly His
63
50-90
Plasma membrane protein, permeabilized ...
Tc: CCPGCC
54
42-78
Intact Cells
Cy3NTAc
Poly His
65
52-94
Plasma membrane protein, permeabilized ...efficiencies between 80% and 10%.
C Cy3NTA and Cy5NTA bind His tags similarly but with differing R0 values, thereby...department at Brigham and Women’s Hospital in Boston, MA. His interests include developing novel fluorescent tools...