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We narrowed to 222 results for: his

Showing: 41 - 60 of 222 results
  1. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

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    Blog Post
    ...As MIT's Ed Boyden explains it, his goal is "to understand the brain at a level of abstraction that enables... fix it when things go awry. To reach that goal, his group develops tools to tinker with brain circuits... article published in Nature Methods, Boyden and his team describe the discovery of a light-sensitive ...optogenetics advance, the big questions that motivate his interest in neuroengineering, and some of the optogenetic...
  2. Tips for Using FRET in Your Experiments

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    Blog Post
    ...optimized CyPet pCyPet-His Cyan Bacterial Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian...optimized YPet pYPet-His Yellow Bacterial Expresses YPet with C-terminal His tag SYFP2 Yellow Mammalian...Cyan Bacterial Expresses Aquamarine with N-terminal His tag pAquaN1 Cyan Mammalian Expresses mammalian ...
  3. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...which can be carried out using Addgene plasmids. His-tag labeling reagents You may already have a FRET...used for protein purification: the NTA binds to the His tag on your protein and the coupled Cy fluorophore... Tc: CCPGCC 50 40-72 Intact Cells Cy3NTAc Poly His  44 35-63 Plasma membrane protein, permeabilized ... Tc: CCPGCC 48 38-70 Intact Cells Cy3NTAc Poly His  63 50-90 Plasma membrane protein, permeabilized ... Tc: CCPGCC 54 42-78 Intact Cells Cy3NTAc Poly His  65 52-94 Plasma membrane protein, permeabilized ...efficiencies between 80% and 10%. C Cy3NTA and Cy5NTA bind His tags similarly but with differing R0 values, thereby...department at Brigham and Women’s Hospital in Boston, MA. His interests include developing novel fluorescent tools...
  4. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ... Children’s Hospital and Harvard Medical School. His lab studies the molecular genetics of blood cell ...cell development and disease. Learn more at his webpage, bauerlab.org.     Matthew Canver is an MD/PhD student...laboratories of Stuart Orkin and Daniel Bauer. He received his BSE in Bioengineering from the University of Pennsylvania...
  5. Celebrating Accomplishments in the Lab

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    Blog Post
    ...cooks and cleans with his family, “sometimes listening to audiobooks.” Church and his family spend family...Ellis highlights the importance of acknowledging his teams successes. “When PhD students graduate there...
  6. How to Negotiate a Successful Lab Start Up Budget

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    Blog Post
    ...instead started a company around his own research. He was clear on his science, and found a solution. However...manageable laboratory environments. His business skills enhance his passion for science. Follow him on ...to provide the resources required for him to meet his goals. Therefore, he turned from academia altogether...
  7. Future of Research Conference - Remarkable Opening Session

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    Blog Post
    ...problems resulting from past practices and mores.  His list is dire:  The current system is unsustainable... be in academia to make change.  He talked about his own unique way of funding basic research at NEB and... of the Novartis industry postdoctoral program.  His strongest comments were about the astounding lack...
  8. Twelve Amazing Guest Blog Contributors!

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    Blog Post
    .... Illuminating Choices Paul Heisig, who shared his flow cytometry expertise in three posts. An Introduction...Serendipity Nicolas Mathis, who shared information on his open software that predicts efficiencies of prime...
  9. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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    Blog Post
    ...first of 19 talks given over the course of the day. His talk, which focused on the history of genome engineering...mentioned specifically in Dr. Zhang's introduction. His willingness to share reagents so freely with the ...analog versions of each, and Tim Lu even compared his "memory" gene codes to a Turing machine (also discussed...
  10. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    Blog Post
    ...about his own biases--many of which will be shared by fellow scientists--but that doesn’t make his introduction...case studies are “old,” they lead Zimmer to one of his most critical observations: “Today the most successful...if Zimmer was able to rewrite the last chapter of his book today--the day you are reading this review--...
  11. Finding and Joining Your Dream Lab

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    Blog Post
    ... successful applicants and get chosen to work in his or her lab of choice? Is the group micromanaged or...to the lab to see how they fit in with the team. His advice is “if you’re within a year from the end of...the lab for a full day, gives a 1 hour talk about his or her interests, and spends one-on-one time with...
  12. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

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    Blog Post
    ...science lab.” Introducing GreenGate As Lohmann and his colleagues describe in the PLoS ONE paper that introduced... it no longer works, just trash it.” Lohmann and his colleagues including Joachim Forner now expect and...
  13. A Primer on Optogenetics: Introduction and Opsin Delivery

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    Blog Post
    ... Dr. Deisseroth has also generously made many of his optogenetic constructs available through the vector... at the University of North Carolina) that offer his constructs. Importantly, control viruses (not expressing...guest blogger Derek Simon! Derek P. Simon received his PhD in Cellular and Molecular Biology from the University...Mary Jeanne Kreek at the Rockefeller University.  His interests include the role of learning and memory...is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org or follow him on Twitter...
  14. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    ...with a CRISPR gene editing therapy personalized to his genetic mutation. “Baby KJ” was diagnosed with a ...rare and dangerous metabolic disease shortly after his birth. Within a seven-month whirlwind of developing...Rebecca Ahrens-Nicklas administered the first dose of his personalized genetic medicine (Musunuru et al., 2025...KJ was born with a C→T mutation on one allele of his CPS1 gene, which could be repaired with an A→G edit...
  15. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...journal Science Signaling in December 2014, he and his colleagues at Duke University, in collaboration with...Applications of the cancer pathways ORFs kit Wood and his colleagues developed the kit for use in screening...
Showing: 41 - 60 of 222 results