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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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In Category

We narrowed to 876 results for: lic

Showing: 41 - 60 of 876 results

  1. Fluorescence Titering Assay

    Type
    Protocol
    ... lentiviral vector allows you to control the multiplicity of infection (MOI) in downstream studies. This... these cells can still be used in downstream applications. Safety Warnings Lentivirus is generally considered...

  2. Lentivirus Production

    Type
    Protocol
    ...lentivirus can be used for a variety of downstream applications such as stable-cell line generation. Workflow...mixture. Add the diluted PEI dropwise while gently flicking the diluted DNA tube. Incubate the mixture 12–...

  3. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...amplify and design primers that will bind to and replicate it. The following image shows the ends of the ...mutation than restriction enzyme based cloning. DNA replication by PCR has error rates that range from roughly...

  4. Western Blot

    Type
    Protocol
    ...BSA standard that range from 0–2000 µg/mL . In duplicate, dilute 10 µL of standard, blank, and lysate samples... nm . Calculate the average absorbance of the duplicate samples on the plate. Subtract the average absorbance...

  5. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...(Lenti_sgRNA_EFS_GFP) 65656 Mammalian/Lentiviral LIC none S. pyogenes GFP Vakoc U6>sgRNA(F+E) 59986 Other...RNA Targeting RNA Targeting RNA Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled...

  6. DNA Quantification

    Type
    Protocol
    ...mini-prepped DNA onto the pedestal. Close the lid and click measure, be sure to record the concentration and...

  9. Centrifugation

    Type
    Protocol
    ...according to speed and time (and temperature, if applicable) listed in your protocol. Pro-Tip Spin speed ...

  10. Gibson Assembly Protocol

    Type
    Protocol
    ...Link opens in a new window) . Gibson Assembly® is licensed to New England Biolabs by TelesisBio. Gibson DG...

  11. General Transfection

    Type
    Protocol
    ...DNA. Add the diluted PEI dropwise while gently flicking the diluted DNA tube. Incubate the mixture 15–...

  13. Immunocytochemistry

    Type
    Protocol
    ...as methanol or acetone may be better for some applications. Remove the paraformaldehyde and follow your...

  14. Colony Formation Titering Assay

    Type
    Protocol
    ...lentiviral preparation allows you to control the multiplicity of infection (MOI) in downstream studies. This...

  18. AAV ddPCR Titration

    Type
    Protocol
    ...quantification. This protocol was modified from the publication Lock et al. (2014) (Link opens in a new window...

  19. AAV Production in HEK293 Cells

    Type
    Protocol
    ... layers are covered with media. 293T cells are delicate and detach very easily - media should always be...

  20. Lentivirus ddPCR Titration

    Type
    Protocol
    ... sample. This protocol was modified from the publication Wang et al. (2018) . Before Starting Thaw the...
Showing: 41 - 60 of 876 results