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...antibody can be purified for use in a variety of applications. Sharing speeds science. We believe that sharing...antibody-expressing plasmids containing the SV40 origin of replication use a HEK293 line stably expressing the SV40 ... containing the Epstein-Barr virus origin of replication use a HEK293 line stable expressing Epstein Barr...

...sufficient quantities of library for experimental applications. Repeated amplifications should be avoided as...required for bacterial propagation (origin of replication and antibiotic selection). This recombination...each 50 µL aliquot of thawed Endura Duos on ice. Flick gently to mix. Electroporate cells (one at a time...

... lentiviral vector allows you to control the multiplicity of infection (MOI) in downstream studies. This... these cells can still be used in downstream applications. Safety Warnings Lentivirus is generally considered...

...lentivirus can be used for a variety of downstream applications such as stable-cell line generation. Workflow...mixture. Add the diluted PEI dropwise while gently flicking the diluted DNA tube. Incubate the mixture 12–...

...amplify and design primers that will bind to and replicate it. The following image shows the ends of the ...mutation than restriction enzyme based cloning. DNA replication by PCR has error rates that range from roughly...

...BSA standard that range from 0–2000 µg/mL . In duplicate, dilute 10 µL of standard, blank, and lysate samples... nm . Calculate the average absorbance of the duplicate samples on the plate. Subtract the average absorbance...

...mini-prepped DNA onto the pedestal. Close the lid and click measure, be sure to record the concentration and...

...of the UV and with a clean, sterile razor blade, slice the desired DNA fragment from the gel. Notes: To...

.... You can find Addgene's sequencing results by clicking on the "View Sequences" link on the Plasmid Information...

...according to speed and time (and temperature, if applicable) listed in your protocol. Pro-Tip Spin speed ...

...Link opens in a new window) . Gibson Assembly® is licensed to New England Biolabs by TelesisBio. Gibson DG...

...DNA. Add the diluted PEI dropwise while gently flicking the diluted DNA tube. Incubate the mixture 15–...

... 3:1 ratio is not working or when doing more complicated cloning. While 3:1 will get you in the ballpark...

...as methanol or acetone may be better for some applications. Remove the paraformaldehyde and follow your...

...lentiviral preparation allows you to control the multiplicity of infection (MOI) in downstream studies. This...

...information on determining DNA concentration and purity click here . Top Protocol: Kit-free Alkaline Lysis Plasmid...

... of a heat resistant DNA polymerase from the thermophilic bacterium, Thermus aquaticus (Taq) . Taq polymerase...

...lower dilutions depending on your downstream applications. If you’ve titered your virus beforehand, you...

...quantification. This protocol was modified from the publication Lock et al. (2014) (Link opens in a new window...

... layers are covered with media. 293T cells are delicate and detach very easily - media should always be...