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We narrowed to 49 results for: luciferase expression

Showing: 41 - 49 of 49 results

  1. Tetracycline Inducible Expression

    Type
    Collection
    ...Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Additional Resources: Browse...subsequent increase in expression by the activation domain, resulting in reduced gene expression. This idea of ... rtTA or TetR expression plasmid Stable cell lines can be made that continuously express a system component...Lentiviral, miRNA expression (PRIME) system for application in knockdown of gene expression at a single copy...copy in mammalian cells; Expresses firefly luciferase hairpin and GFP under pTREtight promoter None Either...promoters capable of controlling eukaryotic gene expression. Several endogenous promoters had been identified...coli presented the best candidates for inducible expression. Bacterial systems were tested for functionality...

  2. Plasmid Collections

    Type
    Collection
    ...Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression Kits Kits ...Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics Chemogenetics Viral Plasmids Lentivirus... Topics Antibody Plasmid Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion...Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic Biology Viral Vectors Communities Find...

  3. Fluorescent Protein Guide

    Type
    Collection
    ... Resources You May Also Like... Luminescence: Luciferase Plasmids Blog: Which FP Should I Use? Blog: How... Proteins from Kurt Thorn and UCSF Molecular Expressions Galleries from Michael Davidson and FSU Spectra...

  4. Chemogenetics Guide

    Type
    Guide
    ...light-emitting luciferase and a light-sensing optogenetic element. When the luciferase substrate (luciferin... Dlx Interneurons EF1a, CAG General expression Targeted expression. Depending on the experiment, an AAV-encoded...cell-specific expression of AAV-encoded chemogenetic receptors. FLEx vectors block expression in the absence...luciferin) is added, the luciferase enzyme generates light that activates the opsin. The opsin may excite...excite or inhibit the neurons expressing the LMO, depending on its biophysical properties. While LMOs provide...Coupling brighter bioluminescent modules, such as luciferase-fluorescent protein fusions, to more-sensitive...increased efficiencies. Depending on the type of luciferase used, the luciferin substrate may be the native...

  5. Cre-lox system

    Type
    Collection
    ...present. By expressing Cre at specific times or locations, you can precisely control expression of your gene...developed ways to tightly control Cre expression and to optimize Cre expression once it is induced. Addgene has...defines the areas in which Cre will be expressed. Cre may be expressed globally under a broadly active promoter... or expressed only in a subset of cells under a more specific promoter (e.g. Rho-Cre is expressed in the...visualization of Cre expression. Optimized Cre: Codon-optimized Cre ( iCre ) is expressed at higher levels...pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Cre, luciferase, and sgRNA expression EFS AAV Zhang 60229 AAV:ITR-U6-sgRNA... EGFP, and sgRNA expression hSyn AAV Zhang 60820 pSECC Cre, Cas9 and sgRNA expression EF-1 alpha Lentiviral...

  6. COVID-19 Resources

    Type
    Collection
    ...SARS-CoV-2 infection. General Tools : Plasmids expressing Cas12a/Cas13a, Reverse Transcriptases, etc. that...Collection - This page includes a table of all spike expression plasmids in our collection that are suitable ...spike pseudotyped virus. It also lists several luciferase and fluorescent reporter plasmids that have been...collection contains: Plasmids for E. coli and Yeast expression Spike protein constructs for making VSV pseudo...Taq polymerase and MMLV reverse transcriptase expression plasmids from the Drew Endy and Philippa Marrack...2020.04.13.039941 (Link opens in a new window) BST-LF expression plasmid from Andrew Ellington's lab. Find plasmid...

  7. Molecular Biology Reference

    Type
    Guide
    ...information. Expression Plasmids - Used for gene expression (for the purposes of gene study). Expression vectors... the level of insert expression (i.e., a strong promoter directs high expression, whereas weaker promoters...amount of protein or RNA produced. Expression vectors can drive expression in various cell types (mammalian...reducing the expression of an endogenous gene. This is frequently accomplished through expression of an shRNA... control of a bacterial promoter, and is thus expressed in the bacteria by bacterial transcriptional machinery...restriction enzymes digestion and ligation. In expression plasmids, the MCS is often located downstream...that when a gene is inserted within the MCS, its expression will be driven by the promoter. As a general ...

  8. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...You may also like... Addgene Blog: Biosensors Luciferase Plasmids Subcellular Localization Optogenetics...Kim , GENIE Project Calcium Bicistronic vector expressing GCaMP6 and mRuby2 for imaging neural activity... Huebener , Tobias Rose Calcium Soma-targeted expression of jGCaMP8f, GCaMP7f, or GCaMP6f Precision Calcium...6. Edward Boyden Calcium Intersectional viral expression of GCaMP6m/s in cells depdent on Cre, Flp, or...Karl Deisseroth , INTRSECT 2.0 Project Calcium Expression of GCaMP6f in PV cortical interneurons under ... Aug 4;11(1):3881. Adam Cohen Calcium GCaMP6f expression in forebrain GABA-ergic interneurons under the... Oct 25;9(1):4440. Eric Schreiter Calcium AAV expression of photoconvertible fluorescent protein-based...

  9. Control AAV Preps

    Type
    Collection
    ...Service: Control AAV Browse our control AAV that express fluorescent proteins under general promoters. These...Constitutive 5, 8 Wilson 105532 pAAV.CMV.ffLuciferase.SV40 CMV ffLuciferase Constitutive 8 Wilson 105536 ...
Showing: 41 - 49 of 49 results