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We narrowed to 50 results for: luciferase expression

Showing: 41 - 50 of 50 results

  1. Bacterial Expression Systems

    Type
    Collection
    ... Plasmid Collections Bacterial Expression Systems Bacterial Expression Systems Protein Purification Protein...bacterial expression systems. Read below and browse our curated collection of bacterial expression plasmids...Addgene Blog E. Coli Strains for Protein Expression Protein Expression Epitope Tags Protein Tags Return to ...to top Controlled Expression It isn’t always advantageous to constitutively express your gene of interest...turn on expression only at a specific time. This collection contains plasmids whose expression levels ...Addgene Controlled Expression Resources Check out our Tetracycline (Tet) Inducible Expression Collection for...transcription factors. Addgene Blog Luciferase Technologies Enabled by NanoLuc® Luciferase Fluorescent Biosensors ...

  2. Plasmid Collections

    Type
    Collection
    ...Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression Kits Kits ...Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics Chemogenetics Viral Plasmids Lentivirus... Topics Antibody Plasmid Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion...Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic Biology Viral Vectors Communities Find...

  3. Fluorescent Protein Guide

    Type
    Collection
    ... Resources You May Also Like... Luminescence: Luciferase Plasmids Blog: Which FP Should I Use? Blog: How... Proteins from Kurt Thorn and UCSF Molecular Expressions Galleries from Michael Davidson and FSU Spectra...

  4. Chemogenetics Guide

    Type
    Guide
    ...light-emitting luciferase and a light-sensing optogenetic element. When the luciferase substrate (luciferin... Dlx Interneurons EF1a, CAG General expression Targeted expression. Depending on the experiment, an AAV-encoded...cell-specific expression of AAV-encoded chemogenetic receptors. FLEx vectors block expression in the absence...luciferin) is added, the luciferase enzyme generates light that activates the opsin. The opsin may excite...excite or inhibit the neurons expressing the LMO, depending on its biophysical properties. While LMOs provide...Coupling brighter bioluminescent modules, such as luciferase-fluorescent protein fusions, to more-sensitive...increased efficiencies. Depending on the type of luciferase used, the luciferin substrate may be the native...

  5. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...genome or control gene expression or skip ahead to browse highlighted plasmids expressing Cre , Flp , Dre ,...Recognition Sites. Controlling Recombinase Expression By expressing the enzyme at specific times or locations...popular recombinase-dependent gene expression strategies. Gene Expression: A stop codon flanked with loxP...return gene expression to physiological levels. In lox-STOP-lox shRNA constructs, Cre expression promotes...toxicity. Leakiness Leaky expression, where the gene of interest is expressed even in the absence of the...catalog for plasmids expressing Cre recombinase and filter the results by expression system and vector type...catalog for plasmids expressing Flp recombinase and filter the results by expression system and vector type...

  6. COVID-19 Resources

    Type
    Collection
    ...SARS-CoV-2 infection. General Tools : Plasmids expressing Cas12a/Cas13a, Reverse Transcriptases, etc. that...Collection - This page includes a table of all spike expression plasmids in our collection that are suitable ...spike pseudotyped virus. It also lists several luciferase and fluorescent reporter plasmids that have been...collection contains: Plasmids for E. coli and Yeast expression Spike protein constructs for making VSV pseudo...Taq polymerase and MMLV reverse transcriptase expression plasmids from the Drew Endy and Philippa Marrack...2020.04.13.039941 (Link opens in a new window) BST-LF expression plasmid from Andrew Ellington's lab. Find plasmid...

  7. Molecular Biology Reference

    Type
    Guide
    ...information. Expression Plasmids - Used for gene expression (for the purposes of gene study). Expression vectors... the level of insert expression (i.e., a strong promoter directs high expression, whereas weaker promoters...amount of protein or RNA produced. Expression vectors can drive expression in various cell types (mammalian...reducing the expression of an endogenous gene. This is frequently accomplished through expression of an shRNA... control of a bacterial promoter, and is thus expressed in the bacteria by bacterial transcriptional machinery...restriction enzymes digestion and ligation. In expression plasmids, the MCS is often located downstream...that when a gene is inserted within the MCS, its expression will be driven by the promoter. As a general ...

  8. Tetracycline Inducible Expression

    Type
    Collection
    ...Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression You may also like... Blog...factors to further minimize background expression. Improved expression of the transactivator proteins in mammalian...Experimental Considerations Expression System If your gene of interest should only be expressed occasionally, use...PB-EF1a-TetOn3G PiggyBac vector expressing Tet-On 3G transactivator constitutively expressed by EF1a promoter Tet-On...Dox-inducible expression of Cre recombinase. Madeline Lancaster 198752 DiLiCre 2.0 Dox-inducible expression of a...Learn about tetracycline-regulated gene expression and find plasmids for your experiments using tet- ... promoters are powerful tools to control gene expression and study biological targets. The tetracycline...

  9. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...You may also like... Addgene Blog: Biosensors Luciferase Plasmids Subcellular Localization Optogenetics...Kim , GENIE Project Calcium Bicistronic vector expressing GCaMP6 and mRuby2 for imaging neural activity... Huebener , Tobias Rose Calcium Soma-targeted expression of jGCaMP8f, GCaMP7f, or GCaMP6f Precision Calcium...6. Edward Boyden Calcium Intersectional viral expression of GCaMP6m/s in cells depdent on Cre, Flp, or...Karl Deisseroth , INTRSECT 2.0 Project Calcium Expression of GCaMP6f in PV cortical interneurons under ... Aug 4;11(1):3881. Adam Cohen Calcium GCaMP6f expression in forebrain GABA-ergic interneurons under the... Oct 25;9(1):4440. Eric Schreiter Calcium AAV expression of photoconvertible fluorescent protein-based...

  10. Control AAV Preps

    Type
    Collection
    ...Service: Control AAV Browse our control AAV that express fluorescent proteins under general promoters. These...Constitutive 5, 8 Wilson 105532 pAAV.CMV.ffLuciferase.SV40 CMV ffLuciferase Constitutive 8 Wilson 105536 ...
Showing: 41 - 50 of 50 results