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We narrowed to 792 results for: post

Showing: 41 - 60 of 792 results

  1. Plant Plasmids and Resources

    Type
    Collection
    ...Promoter PI Return to top Other Resources Addgene Blog Posts Addgene Blog: Plant Biology RUBY-Red Siliques CRISPR-mediated...

  2. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base Edit Catalytically dead dCas9 fused to a cytidine...

  3. Zebrafish Plasmid Collection

    Type
    Collection
    ...proteins. Return to top Other Resources Addgene Blog Posts Advancing Biology with Zebrafish: Genetic Tools ...

  5. CRISPR Plasmids - Plants

    Type
    Collection
    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed...

  6. Fluorescence Titering Assay

    Type
    Protocol
    ...may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service Introduction This... of dilutions of pHAGE-TO-dCas9-3XmCherry . 72 h post transduction, cells were assayed for mCherry expression...

  7. AAV ddPCR Titration

    Type
    Protocol
    ...AAV Viral Service Viral Vector Guides Virus Blog Posts Molecular Biology Reference Introduction This protocol...tips on AAV titering using ddPCR, read this blog post on ddPCR for AAV quantification. This protocol was... tips on AAV titering using ddPCR, read our blog post. Last reviewed on: August 31, 2023...

  8. General Transfection

    Type
    Protocol
    ...may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service Introduction This...production) Day 1: Transfect Cells Day 2 (am): 18 h post transfection - Remove media, replace with fresh ...

  9. Lentivirus Production

    Type
    Protocol
    ...(pm): Transfect packaging cells Day 2 (am): 18 h post-transfection. Remove media, replace with fresh media...cells. Virus can be harvested at 48, 72, and 96 h post transfection in individual harvests or a combined...

  12. Video Library

    Type
    Protocol
    ...for working with tissue culture in the lab. Blog post: 10 Basic tips for mammalian cell culture Aseptic...

  13. Luciferase Plasmid Collection

    Type
    Collection
    ...original publication or read our Luciferase blog post . Empty Backbones Browse or search for popular empty...screening of transformants in cloning process. Lynne Postovit 64034 pGL4Luc-RLuc Firefly, Renilla Dual reporter...

  14. AAV Production in HEK293 Cells

    Type
    Protocol
    ...AAV Viral Service Viral Vector Guides Virus Blog Posts Molecular Biology Reference Introduction This protocol...120 h. Optional: perform a media exchange at ~18 h post transfection. Changing the media can reduce toxicity...

  15. Zhang Lab CRISPR Page

    Type
    Collection
    ... CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo...

  16. Immunocytochemistry

    Type
    Protocol
    ...like... Antibody Plasmid Collection Antibody Blog Posts Antibody Guide Western Blot Protocol Recombinant...

  17. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...in rodents and non-human primates. Read our blog post on systemic capsids for more information. Browse...better understand brain structure. Read our blog post on AAVrg for more information. Self-complementary...more about these and other initiatives in our blog post on using AAV for neuronal tracing . Browse AAV vectors...optimized for their use in rAAV vectors . Read our blog post on overcoming AAV size limitation for CRISPR delivery...development of further AAV applications. Read our blog post on split AAVs for more information. Viral Vector...packaging. WPRE in cis Woodchuck hepatitis virus post‐transcriptional regulatory element; stimulates the...

  18. Plan Your Experiment

    Type
    Guide
    ... and right homology arms. See Addgene's HDR blog post for more details. In some cases, there may be no... aureus Cas9. For more information, see our blog post on overcoming the AAV size limitation . Browse Addgene's...information, take a look at our collection of blog posts about CRISPR expression systems and delivery methods...expression? For more information, check out our blog post on validating CRISPR modifications . Cell Population...

  19. Plasmids for Stem Cell Research

    Type
    Collection
    ...difficult to decide which method to use. Addgene's blog post Delivery Methods for Generating iPSCs provides an...

  20. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR is a useful tool for genetic screening experiments...
Showing: 41 - 60 of 792 results