...Thursday, September 20 6:00 PM-8:00 PM Group Dinner Relax and Connect with Fellow Speakers and Attendees Friday...

...reference (typically ROX dye), to normalize non-PCR–related fluorescence fluctuations and to minimize well-...following: Standard curve: R 2 (coefficient of correlation) ~ 1.0, E (efficiency of PCR) ~100% (90%-110%...

...Intuitively, one would expect that assembling relatively small, repeat-laden DNA regions into a single...

... across the dilutions. RPP30 copies should be relatively constant across samples. In the RRE example below...concentration of RPP30 positive droplets stays relatively even across samples (green). To increase the ...

...to interrogate brain function and examine the relationship between neuronal signaling and behavior. Depending...

...AND NOT Cre Dual recombinase-dependent: Rabies-related Addgene ID Plasmid Logic Sites and Mutations 131779...

...should the double strand break (DSB) be positioned relative to where the homology ends? The Zhang lab has ...

...contained at lower biosafety levels and elicit relatively low immunological effects in vivo . While AAVs...

...years later, PubMed lists more than 6,300 CRISPR-related publications, many of which detail work to improve...

...of orientation of the protospacer sequence/PAM relative to the top or bottom strand. Determine the reverse...

...researchers also use bacteria like E. coli and their relatively well understood biology to answer many interesting...

...Browse neurodegeneration plasmids for research related to Alzheimer's Disease, ALS, Frontotemporal Dementia...collection of neurodegeneration plasmids for research related to Alzheimer's Disease (AD), Amyotrophic Lateral...pSLIK-NFLAG-hFUS FUS Flag, His TRE ALS Zissimos Mourelatos 51146 DsRed-p150 217-548 DCTN1 CMV ALS Trina ...

...in size. The structure of the primer should be relatively simple and contain no internal secondary structure...

... Let's assume that your favorite vector has a relatively limited MCS (BamHI - EcoRI - SalI) and you want...

... your experiment. Water baths tend to heat up relatively slowly. Place the water bath cover on the top...

...protocol. *Pro-Tip* Spin speed is often given as Relative Centrifugal Force (RCF) or Revolutions Per Minute...

...also make their own competent cells. This is a relatively simple procedure and is useful for performing...

... in the liquid. *Pro-Tip* If pipetting from a relatively large container holding a small volume of liquid...

...10 µg/mL. *Pro-Tip* Transducing too many cells relative to the number of virus particles reduces the transduction...

...ImageJ or a similar photo software to determine the relative intensity of the protein bands to the overall ...