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...pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE CamKII ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Karl Deisseroth...pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Karl Deisseroth...pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE EF1a ChRmine (high-photocurrent, red-shifted) mScarlet Cre dependent 1, 5 Karl... 6m-p2A-ChRmine-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) GCaMP6m Constitutive 8 Karl Deisseroth... pAAV-nEF-ChRmine-mScarlet nEF ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Karl Deisseroth...pAAV-nEF-Con/Fon-ChRmine-oScarlet nEF ChRmine (high-photocurrent, red-shifted) oScarlet Cre and Flp dependent...-Coff/Fon-ChRmine-oScarlet nEF ChRmine (high-photocurrent, red-shifted) oScarlet Flp dependent 8 Karl ...

...native UGA recognition. Farren Isaacs Return to top Other Resources and References GCE4All Research Center... directed evolution to charge the tRNA with a different amino acid. To achieve these modifications, two...coli p-acetyl-l-phenylalanine (pAcF) Bacterial TAG Farren Isaacs 73545 pEvol-pAcFRS.1.t1 pAcFRS.1.t1 E. coli...coli p-acetyl-l-phenylalanine (pAcF) Bacterial TAG Farren Isaacs 73546 pEvol-pAzFRS.2.t1 pAzFRS.2.t1 E. coli...coli p-azido-l-phenylalanine (pAzF) Bacterial TAG Farren Isaacs 73547 pEvol-pAzFRS.1.t1 pAzFRS.1.t1 E. coli...coli p-azido-l-phenylalanine (pAzF) Bacterial TAG Farren Isaacs 82417 pUltra-sY sY-specific aaRS M. jannaschii...EAziRS) p-Azido-phenylalanine (Azi) Mammalian TAG Irene Coin 105830 pNEU-hMbPylRS-4xU6M15 PylRS M. barkeri...

...Overview of shRNA-mediated RNA interference. Created with BioRender.com. shRNA Plasmids Browse our selection...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral...Tools for RNAi Browse Plasmids Resources RNA interference (RNAi) is an RNA-mediated gene silencing mechanism...pSico with pSicoligomaker (Andrea Ventura lab) References Carthew, R. W., & Sontheimer, E. J. (2009). Origins...J. (2009). siRNA vs. shRNA: Similarities and differences . Advanced Drug Delivery Reviews, 61 (9), 746... Tuning silence: conditional systems for RNA interference . Nature Methods, 3 (9), 682–688. PubMed (Link...

...Beta-catenin GFP Adherens junctions beta-Catenin EGFP Alpha Yap 67937 mouse E-cadherin GFP (423) Adherens junctions...interest with a fluorescent protein that has a different emission wavelength. For example, if the marker...mGreenLantern Gregory Petsko 221898 mito-mStayGold_pTwistCMVBetaGlobWPRENeo Mitochondria Mitochondrial targeting...mGreenLantern Gregory Petsko 221899 mStayGold-aTubulin_pTwistCMVBetaGlobWPRENeo Microtubules alpha-Tubulin mStayGold...stress. There are four types of cell junctions: adherens junctions, gap junctions, tight junctions, and...EGFP Alpha Yap 71366 Murine E-cadherin mCherry Adherens junctions E-cadherin mCherry Alpha Yap 55114 mCherry-Paxillin...Michael Davidson 55001 mCherry-Beta-Catenin-20 Adherens junctions beta-Catenin mCherry* Michael Davidson...

...Phosphoprotein Reagents Phosphoprotein Synthesis Reagents References & Protocols Additional Resources Figure 1: SepRS...amino acids on either side occurring within the parent protein. Using the recoded strain of E. coli , ...performing phosphoproteomic experiments in need of reference spectra or phosphopeptide standards for quantitative...The Mode #2 phosphosite library contains four different phosphobinding domains: 14-3-3β, 14-3-3σ, the ...phosphorylation positional isomers are allocated to different subpools, allowing precise knowledge of phosphorylated... SepOTSα SepRS/EF-Sep ( aka. pKD-SepRS-EFSep) References & Protocols Iterative Synthetically Phosphorylated...Umehara, T., Guo, L., Osborne, E. M., Benner, J., Noren, C. J., Rinehart, J., & Söll, D. (2011). Expanding...

...Overview of CRISPR interference. Browse, sort, or search the tables below for CRISPR interference plasmids. To...expression by interfering with transcription. This interference is most efficient when the gRNA targets your... To learn more about CRISPR interference and other CRISPR topics, read our CRISPR Guide . Mammalian ID...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...

...express both components in the same relative ratio. Different generations of transactivators and promoters are...new window) for comparison of Tet-On systems in different applications. Use of Tetracycline or a Derivative...172115 PB-TO-hNGN2 Piggybac Tet-On plasmid for differentiating iPSCs into glutamatergic neurons via human ...NGN2 into AAVS1 safe harbor site, for iPSC differentiation into cortical neurons Michael Ward 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA...Inducible Promoters Blog: Repressible Promoters References Das, A. T., Tenenbaum, L., & Berkhout, B. (2016...window) Krueger, C., Pfleiderer, K., Hillen, W., & Berens, C. (2004). Tetracycline derivatives: alternative...

...Constitutive rg* Loren Looger 116870 pAAV-CAG-H2B tdTomato CAG H2B-tdTomato Constitutive rg* Loren Looger 105530...These AAV can be used to compare the activity of different serotypes. Serotype AAV1 AAV2 AAV5 AAV6 AAV8 AAV9... GFPsm_myc (does not fluoresce) Cre dependent 1 Loren Looger 98928 pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40...Ruby2sm-Flag (does not fluoresce) Cre dependent 1, 5 Loren Looger 45185 AAV-EF1a-BbTagBY EF1a TagBFP and EYFP...

...generated between the target sites. Created with BioRender.com. Scientists have adapted these systems to activate...enzyme is present. Read on to learn about the different ways that Cre-lox and other site-specific recombinases... 14 bp inverted repeats + 4 bp spacer (32 bp) Currently, only the canonical rox site is widely used. Flp...C-terminal fragments and placed under the control of different promoters. Expression of both fragments in the...of the orientation specificity of Cre and the different types of target sites available, both mutant and...

...Plasmid Collection INTRSECT Implementation Plasmids References The development of powerful molecular reagents...pAAV-Ef1a-Con/Fon/Von-GCaMP6M Cre AND Flp AND Vcre No References Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee...Conzelmann KK, Staiger JF. 2019. Mapping Brain-Wide Afferent Inputs of Parvalbumin-Expressing GABAergic Neurons...neurons. Preprint (Link opens in a new window) Yu K, Ahrens S, Zhang X, Schiff H, Ramakrishnan C, Fenno L, ...Lazaridis I, Tzortzi O, Weglage M, Märtin A, Xuan Y, Parent M, Johansson Y, Fuzik J, Fürth D, Fenno LE, Ramakrishnan...

...all the antibodies currently available from our catalog. Addgene values transparency and accessibility ...recommended for an application or species. We currently assess western blot, immunohistochemistry, and...should be added to this collection? Check out our current collection of Antibody Plasmids , fill out our ...

...both safety and efficiency. Learn more about the different types of viral vectors and find viral production...Viral Vector Type Each viral vector type exhibits different properties and are suited for specific research...for research and gene therapy. Created with BioRender.com. Wild-type viruses have one goal, which is to...Specific modifications to these plasmids, such as different promoters or tags, can even direct delivery of...

...migration, survival, adhesion, growth, and differentiation. Schematic of Ras signaling cascade that starts...ultimately turn on genes involved in cell growth, differentiation and survival. Ras is essential for regulating...Growth factor receptor bound protein 2 ICMT Isoprenylcysteine carboxyl methyltransferase INSR Insulin receptor...Initiative - at the National Cancer Institute (NCI) References Ras oncogenes and their downstream targets. Rajalingam...

... to target different cell populations in many brain regions. See More iPSC Differentiation Factors Find...Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other...of these are due to a mutation in one of three different inherited genes: Presenilin 1, Presenilin 2, and...

...throughput over traditional tagging methods. Several different tagging techniques, as well as the plasmids and...plasmids for a CRISPR-based system to add a tag (currently using FLAG) to endogenous proteins. This CRISPR... in each row. If your own gene of interest is currently unavailable, you will need to design and clone...Note that the principle of the PITCh system is different from the conventional HDR-mediated gene knock-...

...replication in many gram-negative bacteria with different purification tag combinations, including PelB ...of small molecules, light, and temperature, in different bacterial systems. ID Plasmid Promoter Inducer...contains specific bacterial promoters that vary in strength and that can be used to reliably drive expression...Fusobacterium nucleatum Jörg Vogel 46002 pGR Terminator strength Fluorescence (GFP:mRFP1 ratio) Escherichia coli...

...the genome based on their differential chromatin accessibility across different cell types and cloned into...plasmids to target a variety of cell populations in different brain regions. Labeled cell populations were tested...Kalmbach, B., Lenz, G. H., Morin, E., Nguyen, T. N., Garren, E., Bendrick, J. L., Kim, T. K., Zhou, T., Mortrud...

...heritable in dividing cells. CRISPR activation and interference technologies also indirectly modify local chromatin...epigenetic modification using CRISPR. Created with BioRender.com. Browse, sort, or search the tables below for...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...

...resistance. Protocols & References A transcription factor atlas of directed differentiation. Joung J, Ma S, Tay...The Human TFome Library Stem Cells Protocols & References MORF Plasmids MORF Pooled Library The MORF library...

...the simplicity of gRNA design, programmable for different genomic loci, capable of detecting multiple genomic...visualizing genomic loci using CRISPR. Created with BioRender.com. Browse, sort, or search the tables below for...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...