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We narrowed to 303 results for: rigi

Showing: 41 - 60 of 303 results

  1. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...high-copy number bacterial ColE1 origin with the RSF1010 origin, an IncQ origin with broad host-range (Meyer...suite of plasmids (pGT) with various replicative origins that can be used for broad or narrow range of microbial...microbial species. These also contain the RP4 transfer origin as well as a selectable marker and a desired genetic...

  2. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...flexibility to the CRISPR editing world. The approach, originally published in Nature, is a “search-and-replace...HDR is typically less than 10%. In contrast, the original paper introducing prime editing demonstrated ~...into one strand, there’s a mismatch between the original sequence on one strand and the edited sequence...indels by 13-fold (Anzalone et al., 2019). PE3, as originally introduced, relies on the PE2 enzyme. PE4 and...and DNA ligases to act first. PE4 and PE5, as originally introduced, rely on the PE2 enzyme. PEmax: Optimizing... plasmids from David Liu's lab! This post was originally written by Jennifer Tsang in October 2019 and...

  3. 6 Tips for Grant Writing

    Type
    Blog Post
    ... the deadline isn’t going to be much use. 2. Originality is essential for grant success The two most important...be looking for in your research proposal are originality and relevance. When reading through your proposal...our own or other groups, but your work must be original (otherwise why would you be interested in it?)...Frontier Science Program's website. One way to add originality to your work is to collaborate with specialists... applications to solve problems will provide originality to your work and possibly a new perspective on...

  4. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ... of these CRISPR systems. The original pCas/pTargetF system Our original pCas/pTargetF system consists...Originally published Apr 13, 2021 and last updated Aug 12, 2021. This post was contributed by guest bloggers... to yield pEcCas. In pTargetF, we replaced the original 20 bp specific sequences on pTargetF with the ...of the pEcCas/pEcgRNA system Compared with the original system, the pEcCas/pEcgRNA is superior in several...

  5. The Challenges of Cell Culture

    Type
    Blog Post
    ...history of the cell line consisting of records of the origin and propagation of the line, and can include assays...isoenzymes and genetic polymorphisms specific to their origins. This technique is still used today to test for...researchers are free of contamination and match their original description. The oldest, largest, and most diverse...This is to ensure the cells are as close to the original as possible and so any results generated are reproducible...you can retain the maximum stocks and use of the original Cell line you purchased, keeping all experiments...

  6. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...plant species. Thus, binary vectors contain two origins of replication, one each for E. coli and Agrobacterium...borders to 25 bases each. By switching the E. coli origin of replication and codon-optimizing the Agrobacterium...interest with a constitutive, robust M24 promoter. Originally isolated from the Mirabilis mosaic virus, the...Synthetic Biology, MoClo, and More Plasmids 101: Origin of Replication    ...

  7. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...domain unfolds allowing it to bind to SspB. This original Light Inducible Dimer (oLID) could be used to ...routinely reaches levels 20-40x greater than the original dCas9-VP64 activator. They also revealed that ...enzymes, such that the ligation regenerates the original restriction sites for further iterative cloning... use of electrodes. The Cohen lab has made the original Arch indicator (pJMK004), as well as the CheRiff...Nano-lantern fusions are designed to complement the original yellow variant, with all 3 having distinct emission... the high rate of read-through transcription originating from cryptic promoters in the plasmid backbone...design pXPG, the authors incorporated a high-copy origin of replication and a modified luciferase gene into...

  8. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...Originally published Jan. 14, 2014 and updated Apr. 14, 2020. Any newcomer who joins a molecular biology...and/or virulence. All natural plasmids contain an origin of replication (which controls the host range and...another cell. Minimally, lab-created plasmids have an origin of replication, selection marker, and cloning site...described below.   Vector Element Description Origin of Replication (ORI) DNA sequence which allows ...

  9. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...accessibility of CRISPR-based phenotypic screen datasets. Original published CRISPR screen data is curated and deposited... Each screen curated in ORCS is linked to the original publication via its PubMed identifier, which can...metadata.   Screen results are reported as the original quantitative gene-level scores provided by authors...rather than sgRNA-level sequence read scores. The original supplemental data tables available with the publication...

  10. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    ...Di Ventura (@barbara_synbio) May 13, 2021 The original AraC transcriptional regulator Their system is...light-inducible dimerization domain, Vaucheria frigida aureochrome1 (VfAu1), led to functional BLADE variants...does BLADE have over AraC? When compared to the original AraC system, BLADE is simply more precise. “We...light switch and shuts off just as quickly. The original AraC system, which uses arabinose, requires many...

  11. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

    Type
    Blog Post
    ... another organism in the lab. Predicting lab-of-origin of engineered DNA This isn’t the only time researchers...Addgene plasmid datasets) to predict the lab-of-origin of engineered DNA. Both of these groups have generated...biosurveillance tool,” Kunjapur says. Predicting lab-of-origin and synthetic vs. natural gene are essential and... A. Voigt. "Deep learning to predict the lab-of-origin of engineered DNA." Nature communications 9.1 (...

  12. Hot Plasmids February 2024

    Type
    Blog Post
    ... photostability and brightness compared to the original, though its maturation is even faster. Published...maintaining the bright and stable fluorescence of the original (Ivorra-Molla et al., 2023). They also named their...collection includes a mouse and a chimeric rabbit (original mouse Fab region with rabbit Fc) anti-FLAG antibody...antibodies for use in western blots, though the original parent clone (M2) has been widely used in a variety...

  13. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...of Cas13a. The origins of Cas13a: An RNA cleaving CRISPR nuclease Cas13a was originally identified in ... whether the target sequence is present in the original pool of nucleotides.   What are the potential...the applications of CRISPR. Since this post was originally published, we've also seen the new development...

  14. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...broad host range. These features are: The plasmid origin of replication (ori) contains structural elements...For example, the pJD4 plasmid harbors distinct origins (ori1, ori2, ori3) and replication initiation proteins...Konieczny, I., & Helinski, D. R. (1998). Replication Origin of the Broad Host Range Plasmid RK2: Positioning...More resources on the Addgene blog Plasmids 101: Origin of Replication Plasmids 101: What is a Plasmid?...

  15. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...turning transcription OFF. Despite their bacterial origins, Tet systems function well in mammalian cells, ... previous post. In the past 30 years since the original Tet-Off system was described by Gossen and Bujard...inducible manner detailed in our previous post. While originally developed for use in mammalian cells, this system...work best for your experiments! This post was originally written by Mary Gearing in 2018, and updated ...

  17. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...other contains the bacterial selection marker and origin of replication. During bacterial culture, only ...plasmid with the antibiotic resistance gene and origin will replicate, and the transgene will be lost....contains the antibiotic resistance gene (AmpR) and origins of replication (ori and f1 ori) which allow it ...

  18. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...put down a basic outline and refer back to the original paper that everyone in your lab has been referencing... down to inadequate information about what the original researchers actually did. Admittedly, the differences...President of Editorial at Cell Press.  She trained originally in cell biology, completing her PhD at the MRC...

  19. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...cell type they are tranfected into – a bacterial origin of replication (ORI) will not allow for plasmid...considered an entirely new cell line from that of the original parental cells. Positive selection in mammalian... 101: Antibiotic Resistance Genes Plasmids 101: Origin of Replication All Plasmids 101 Articles ...

  20. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ... signals from time to time. False positives in original studies and false negatives in replications are...replication study. An important difference between the original setting and the new one, previously unaccounted...when replications do not come out the same as an original research finding we can be more certain that some...
Showing: 41 - 60 of 303 results