We narrowed to 169 results for: tox

...DNA sequence and has been suggested to cause cytotoxicity [5]. Second, instead of using a donor-plasmid...plasmids in some cell lines, most likely due to DNA toxicity. More importantly, unlike Cre recombinase, the...“Recombination between inverted loxP sites is cytotoxic for proliferating cells and provides a simple ...

...plasmids with a cargo that may reduce titer due to toxicity or other reasons For these cases, Addgene will...

...fluorescence. Since emission of green light is more phototoxic to cells, most scientists working on in vivo ...fluorescence when exposed to blue light, which is less phototoxic to live tissue than the UV light used by other... a reduced potential for photobleaching and phototoxicity that standard FPs might induce though their ...

...Entamoeba histolytica Leishmania sp. Plasmodium sp. Toxoplasma gondii Trypanosoma sp. Plasmids for Viruses Species...

...Cre-dependent Cre-dependent expression of diphtheria toxin receptor fused to GFP for studying cell ablation...

...apicomplexan organism Toxoplasma gondii to the infection of human fibroblasts. Toxoplasma mutants were generated...Mülleder M, et al. F1000Res. PubMed PMID: 27830062 Toxoplasma CRISPR knockout pooled library Parasites from... and livestock diseases such as malaria and toxoplasmosis. Despite their importance to global health, ...

...homolog RAC RAC1 RAC2 RAC3 Ras-related C3 botulinum toxin substrate (rho family, small GTP binding protein...

...these DNA samples must also be entirely endotoxin free. Endotoxin is a lipopolysaccharide of the cell wall...

...and auxotrophy) and negative selection (ex: toxin-antitoxin systems and sacB counter-selection) tools used...

...of Pharmacology and Toxicology 55:399–417 . https://doi.org/10.1146/annurev-pharmtox-010814-124803 Vlasov...

...can increase the risk of off-target cutting or cytotoxicity, but naturally-occurring Acr (Anti-CRISPR) proteins...including resistances to chemotherapy drugs and toxins, cell viability, and tumor metastasis. One of the...

...want to make a lot of your plasmid? Is the gene toxic in high amounts? It is always good to keep in mind...they contain large inserts or genes that create a toxic product. The E. coli strain Most E. coli strains...

...µg of DNA into 500 µL of Opti-Pro SFM. Pro-Tip Endotoxins can inhibit transfection; therefore, plasmid ...plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, the plasmid...

... Background Lower Higher Photobleaching/phototoxicity Not susceptible Susceptible Subcellular imaging...live-cell imaging by avoiding autofluorescence and phototoxicity and even allowing multiple proteins to be visualized...

...using this plasmid Cas9 expression is typically cytotoxic, which makes generation of high titer Cas9 lentiviruses...lentiviruses challenging. This cytotoxicity also causes growth rate variability when using lentiCas9-blast...

...advancement over previous chemical versions with toxicity to many organisms. Fig. 1: Exogenous sources... to local dsRNA production. In the same vein, genotoxic stress, such as chemotherapy treatment or ionizing...

...spatial precision of gene expression and reduces toxicity as compared to previous Cre/lox or drug-induced...inactivating Cre. This is critical for reducing Cre toxicity in long-term experiments.  Figure 4: The...

...demonstrated that it can also detect expression of toxin mRNA from Clostridium difficile. As mRNA expression...methods, the ability to detect mRNA transcripts of toxins and other transcripts of interest is a benefit ...

... cell toxicity. Cas7-11 does not display collateral RNA cleavage and is significantly less toxic to mammalian...

... optimized for each transfer plasmid. Pro-Tip Endotoxins can inhibit transfection; therefore, plasmid ...plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, the plasmids...