...fluorescence. Since emission of green light is more phototoxic to cells, most scientists working on in vivo ...fluorescence when exposed to blue light, which is less phototoxic to live tissue than the UV light used by other... a reduced potential for photobleaching and phototoxicity that standard FPs might induce though their ...

...apicomplexan organism Toxoplasma gondii to the infection of human fibroblasts. Toxoplasma mutants were generated...Mülleder M, et al. F1000Res. PubMed PMID: 27830062 Toxoplasma CRISPR knockout pooled library Parasites from... and livestock diseases such as malaria and toxoplasmosis. Despite their importance to global health, ...

...these DNA samples must also be entirely endotoxin free. Endotoxin is a lipopolysaccharide of the cell wall...

...and auxotrophy) and negative selection (ex: toxin-antitoxin systems and sacB counter-selection) tools used...

...of Pharmacology and Toxicology 55:399–417 . https://doi.org/10.1146/annurev-pharmtox-010814-124803 Vlasov...

...want to make a lot of your plasmid? Is the gene toxic in high amounts? It is always good to keep in mind...they contain large inserts or genes that create a toxic product. The E. coli strain Most E. coli strains...

...using this plasmid Cas9 expression is typically cytotoxic, which makes generation of high titer Cas9 lentiviruses...lentiviruses challenging. This cytotoxicity also causes growth rate variability when using lentiCas9-blast...

...demonstrated that it can also detect expression of toxin mRNA from Clostridium difficile. As mRNA expression...methods, the ability to detect mRNA transcripts of toxins and other transcripts of interest is a benefit ...

...spatial precision of gene expression and reduces toxicity as compared to previous Cre/lox or drug-induced...inactivating Cre. This is critical for reducing Cre toxicity in long-term experiments.  Figure 4: The...

...advancement over previous chemical versions with toxicity to many organisms. Fig. 1: Exogenous sources... to local dsRNA production. In the same vein, genotoxic stress, such as chemotherapy treatment or ionizing...

... cell toxicity. Cas7-11 does not display collateral RNA cleavage and is significantly less toxic to mammalian...

...cells remain difficult to genetically edit due to toxicity from electroporation or poor cellular entry with...frequency (nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines such as...

...extremely useful if a desired protein product is toxic to cells at high concentrations. Despite their advantages...lower expression may be required if your protein is toxic to E. coli. Of all the vectors, pET, under the control...

...increased particle stability; however, the protein is cytotoxic precluding the long-term expression of lentiviral...vectors in producer cell lines. Identifying less toxic envelope proteins or envelope proteins that target...

...tagRFP Ebert pSAG1::CAS9-U6::sgUPRT 54467 Other/Toxoplasma gondii none, Q5 mutagenesis yes, cut S. pyogenes...pyogenes Sibley pU6-Universal 52694 Other/Toxoplasma gondii BsaI yes, cut S. pyogenes Lourido pRGE31 50929...

...correct? Is the purified plasmid DNA viable? Or cytotoxic? How and where did things go wrong? The use of...reagents or the transfection process itself has any cytotoxic effects on the target cells. Another type of transfection...

...µg of DNA into 500 µL of Opti-Pro SFM. Pro-Tip Endotoxins can inhibit transfection; therefore, plasmid ...plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, the plasmid...

...produce viral proteins and die, either through cytotoxicity or the immune response. The second strategy ...expression. In cell lines that can produce HIV-1 toxic proteins, CRISPR/Cas9 SAM caused apoptosis, indicating...

... optimized for each transfer plasmid. Pro-Tip Endotoxins can inhibit transfection; therefore, plasmid ...plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, the plasmids...

...problematic because excitation light can cause phototoxicity, autofluorescence, and perturb biological phenomena...the case of exciting cyan-excitable donors, phototoxicity. Another challenge to using FRET sensors comes...