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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 172 results for: tox

Showing: 41 - 60 of 172 results

  1. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ... handled at BSL-1, AAVs expressing oncogenes or toxins should be handled at BSL-2. AAV can transduce both...non-dividing cells with a low immune response and low toxicity. Although recombinant AAV does not integrate into...

  2. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...apicomplexan organism Toxoplasma gondii to the infection of human fibroblasts. Toxoplasma mutants were generated...Mülleder M, et al. F1000Res. PubMed PMID: 27830062 Toxoplasma CRISPR knockout pooled library Parasites from... and livestock diseases such as malaria and toxoplasmosis. Despite their importance to global health, ...

  3. Lentivirus Plasmids

    Type
    Collection
    ...particle) and toxicity of the gene product (i.e., if the gene encoded by the transfer plasmid is toxic to mammalian...

  7. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...want to make a lot of your plasmid? Is the gene toxic in high amounts? It is always good to keep in mind...they contain large inserts or genes that create a toxic product. The E. coli strain Most E. coli strains...

  8. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ... Background Lower Higher Photobleaching/phototoxicity Not susceptible Susceptible Subcellular imaging...live-cell imaging by avoiding autofluorescence and phototoxicity and even allowing multiple proteins to be visualized...

  9. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...using this plasmid Cas9 expression is typically cytotoxic, which makes generation of high titer Cas9 lentiviruses...lentiviruses challenging. This cytotoxicity also causes growth rate variability when using lentiCas9-blast...

  10. All in a Twist: dsRNA

    Type
    Blog Post
    ...advancement over previous chemical versions with toxicity to many organisms. Fig. 1: Exogenous sources... to local dsRNA production. In the same vein, genotoxic stress, such as chemotherapy treatment or ionizing...

  13. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...demonstrated that it can also detect expression of toxin mRNA from Clostridium difficile. As mRNA expression...methods, the ability to detect mRNA transcripts of toxins and other transcripts of interest is a benefit ...

  14. Hot Plasmids February 2024

    Type
    Blog Post
    ...cells remain difficult to genetically edit due to toxicity from electroporation or poor cellular entry with...frequency (nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines such as...

  15. Tetracycline Inducible Expression

    Type
    Collection
    ... may be needed. Or, when inducing a potentially toxic protein you may need extremely minimal background...and tTA. Dox has good tissue distribution, low toxicity, a known half-life (24 hours), and is relatively...

  16. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...extremely useful if a desired protein product is toxic to cells at high concentrations. Despite their advantages...lower expression may be required if your protein is toxic to E. coli. Of all the vectors, pET, under the control...

  17. General Transfection

    Type
    Protocol
    ...µg of DNA into 500 µL of Opti-Pro SFM. Pro-Tip Endotoxins can inhibit transfection; therefore, plasmid ...plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, the plasmid...

  18. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...increased particle stability; however, the protein is cytotoxic precluding the long-term expression of lentiviral...vectors in producer cell lines. Identifying less toxic envelope proteins or envelope proteins that target...

  19. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...correct? Is the purified plasmid DNA viable? Or cytotoxic? How and where did things go wrong? The use of...reagents or the transfection process itself has any cytotoxic effects on the target cells. Another type of transfection...

  20. Lentivirus Production

    Type
    Protocol
    ... optimized for each transfer plasmid. Pro-Tip Endotoxins can inhibit transfection; therefore, plasmid ...plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, the plasmids...
Showing: 41 - 60 of 172 results