We narrowed to 512 results for: trac

...phenol-chloroform extraction - see protocol below . Note: Phenol-chloroform extraction removes remaining...-50 μL of TE. Top Protocol: Phenol-Chloroform Extraction of DNA Samples Add an equal volume of TE-saturated...it is advisable to perform a phenol/chloroform extraction of the supernatant after step 6 and before step...

...blue to red over time, allowing tracking of processes such as intracellular trafficking or cell cycle dynamics...

...generation of shRNA transcripts. cPPT Central polypurine tract, cPPT, improves transduction efficiency by facilitating...competent cells Invitrogen #18258-012 Qiaquick gel extraction kit Qiagen #28704 Low melting point agarose Sigma...at 37°C for 2 hours. Purify with Qiaquick gel extraction kit. Elute in 30 μL of ddH 2 O. Digest eluate... the DNA. Purify the DNA using a Qiaquick gel extraction kit. Elute in 30 μL of ddH 2 O. Measure the DNA...AB01200-02000, LB contains: 10g tryptone 5g yeast extract 10g sodium chloride 15g agar Prepare LB agar solution...

...pAAV-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G...pAAV-TREtight-mTagBFP2-B19G helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA...

...with an empty tube. Alternatively, you can just subtract the weight of the empty tube from the weight of...the (Link opens in a new window) QIAquick Gel Extraction Kit . Always follow the manufacturer's instructions...

... COVID-19 resource collections. Protocol: RNA Extraction Without A Kit...

...experimental procedures, such as minipreps and RNA extractions. Plus, centrifuges are useful for simply collecting...cousins, but can hold much larger containers. Ultracentrifuges are also generally floor sized centrifuges...

...Mitogen-activated protein kinases (MAPKs), also known as extracellular signal-regulated kinases (ERKs), are serine/...

...Monitor small biomolecules or other physiological intracellular processes with genetically encoded fluorescent...

... glass bottle: 4 g NaCl 4 g Tryptone 2 g Yeast Extract and dH 2 O to 400 mL Note: If your lab has pre-...mL Kanamycin 50 µg/mL Spectinomycin 50 µg/mL Tetracycline 10 µg/mL Tips and FAQ What is the difference...

...fluorescent CRISPR system has been used for dynamic tracking of repetitive and non-repetitive genomic loci,...

...experiments by removing the amplification and extraction steps required when one receives plasmids in ...

... other difficult-to-target antigens that are intractable via animal immunization. Addgene's antibody catalog...

... a fluorescent protein to the opsin in order to track its expression and localization. Many other types...

...repeat motifs (squares), and also carrying the tracrRNA sequence (not shown). (B) Relative GFP and RFP...

...protein barcodes enable self-correcting neuron tracing with nanoscale molecular context . bioRxiv 2025.09.26.678648...

... other difficult-to-target antigens that are intractable via animal immunization. IPI is a nonprofit research...

...William Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in...

... g pre-mixed powder consisting of: 5.0 g yeast extract 10.0 g peptone from casein 10.0 g sodium chloride.../mL 50 µg/mL Spectinomycin 50 mg/mL 50 µg/mL Tetracycline 10 mg/mL 10 µg/mL Notes: Unless otherwise indicated...

... a sonication step may be required for hard to extract proteins such as those in the nucleus. Incubate...absorbance of the duplicate samples on the plate. Subtract the average absorbance of the blank from all of...