...be lysed or the substrate must be delivered intracellularly in order to measure luminescence. Gaussia luciferase...Resonance Energy Transfer (BRET) CalFlux VNT : An intracellular calcium sensor created by fusing NanoLuc® and...

...pAAV-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G...pAAV-TREtight-mTagBFP2-B19G helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA...

...features using sequence analysis RNA Extraction Without a Kit Extract RNA from tissues or cells without ...! DNA Purification Miniprep, phenol-chloroform extract, and precipitate DNA DNA Quantification Measure...choice AAV Purification by Iodixanol Gradient Ultracentrifugation Purify adeno-associated virus from a preparation...

...phenol-chloroform extraction - see protocol below . Note: Phenol-chloroform extraction removes remaining...-50 μL of TE. Top Protocol: Phenol-Chloroform Extraction of DNA Samples Add an equal volume of TE-saturated...it is advisable to perform a phenol/chloroform extraction of the supernatant after step 6 and before step...

...generation of shRNA transcripts. cPPT Central polypurine tract, cPPT, improves transduction efficiency by facilitating...competent cells Invitrogen #18258-012 Qiaquick gel extraction kit Qiagen #28704 Low melting point agarose Sigma...at 37°C for 2 hours. Purify with Qiaquick gel extraction kit. Elute in 30 μL of ddH 2 O. Digest eluate... the DNA. Purify the DNA using a Qiaquick gel extraction kit. Elute in 30 μL of ddH 2 O. Measure the DNA...AB01200-02000, LB contains: 10g tryptone 5g yeast extract 10g sodium chloride 15g agar Prepare LB agar solution...

...Mitogen-activated protein kinases (MAPKs), also known as extracellular signal-regulated kinases (ERKs), are serine/...

...use to scientists. Addgene's Protocol for RNA Extraction Without A Kit...

...fluorescent CRISPR system has been used for dynamic tracking of repetitive and non-repetitive genomic loci,...

...Monitor small biomolecules or other physiological intracellular processes with genetically encoded fluorescent...

... other difficult-to-target antigens that are intractable via animal immunization. Addgene's antibody catalog...

... other difficult-to-target antigens that are intractable via animal immunization. IPI is a nonprofit research...

... a fluorescent protein to the opsin in order to track its expression and localization. Many other types...

... : Generates crRNA for use in combination with tracrRNA. 51434 pHD-DsRed : vector for generating dsDNA...

...repeat motifs (squares), and also carrying the tracrRNA sequence (not shown). (B) Relative GFP and RFP...

...experiments by removing the amplification and extraction steps required when one receives plasmids in ...

...Synthetic Biology Articles - Peccoud lab social media tracker Synthetic Biology - Open access journal Do you ...

...resistance. Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in...

...consists of 959 somatic cells, which makes them attractive for imaging and fluorescence microscopy experiments...

...experiments. Shipping 2–7 days We will email you with tracking information as soon as your order ships. Shipping...

...Spang, A., Dacks, J. B., & Ettema, T. J. (2016). Tracing the archaeal origins of Eukaryotic Membrane-Trafficking...