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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 517 results for: trac

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Showing: 41 - 60 of 517 results

  1. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...features using sequence analysis RNA Extraction Without a Kit Extract RNA from tissues or cells without ...! DNA Purification Miniprep, phenol-chloroform extract, and precipitate DNA DNA Quantification Measure...choice AAV Purification by Iodixanol Gradient Ultracentrifugation Purify adeno-associated virus from a preparation...

  2. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...phenol-chloroform extraction - see protocol below . Note: Phenol-chloroform extraction removes remaining...-50 μL of TE. Top Protocol: Phenol-Chloroform Extraction of DNA Samples Add an equal volume of TE-saturated...it is advisable to perform a phenol/chloroform extraction of the supernatant after step 6 and before step...

  4. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...generation of shRNA transcripts. cPPT Central polypurine tract, cPPT, improves transduction efficiency by facilitating...competent cells Invitrogen #18258-012 Qiaquick gel extraction kit Qiagen #28704 Low melting point agarose Sigma...at 37°C for 2 hours. Purify with Qiaquick gel extraction kit. Elute in 30 μL of ddH 2 O. Digest eluate... the DNA. Purify the DNA using a Qiaquick gel extraction kit. Elute in 30 μL of ddH 2 O. Measure the DNA...AB01200-02000, LB contains: 10g tryptone 5g yeast extract 10g sodium chloride 15g agar Prepare LB agar solution...

  5. Brain Initiative Collection

    Type
    Collection
    ...pAAV-syn-FLEX-splitTVA-EGFP-tTA helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G...pAAV-TREtight-mTagBFP2-B19G helper virus for monosynaptic tracing; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA...

  6. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...with an empty tube. Alternatively, you can just subtract the weight of the empty tube from the weight of...the (Link opens in a new window) QIAquick Gel Extraction Kit . Always follow the manufacturer's instructions...

  8. Centrifugation

    Type
    Protocol
    ...experimental procedures, such as minipreps and RNA extractions. Plus, centrifuges are useful for simply collecting...cousins, but can hold much larger containers. Ultracentrifuges are also generally floor sized centrifuges...

  9. MAPK Plasmids

    Type
    Collection
    ...Mitogen-activated protein kinases (MAPKs), also known as extracellular signal-regulated kinases (ERKs), are serine/...

  10. Fluorescent Protein Guide

    Type
    Collection
    ...Monitor small biomolecules or other physiological intracellular processes with genetically encoded fluorescent...

  11. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ... glass bottle: 4 g NaCl 4 g Tryptone 2 g Yeast Extract and dH 2 O to 400 mL Note: If your lab has pre-...mL Kanamycin 50 µg/mL Spectinomycin 50 µg/mL Tetracycline 10 µg/mL Tips and FAQ What is the difference...

  13. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...experiments by removing the amplification and extraction steps required when one receives plasmids in ...

  14. All Antibodies

    Type
    Collection
    ... other difficult-to-target antigens that are intractable via animal immunization. Addgene's antibody catalog...

  15. Optogenetics Plasmid Collection

    Type
    Collection
    ... a fluorescent protein to the opsin in order to track its expression and localization. Many other types...

  17. E11 Bio PRISM Collection

    Type
    Collection
    ...protein barcodes enable self-correcting neuron tracing with nanoscale molecular context . bioRxiv 2025.09.26.678648...

  18. Retrovirus Plasmids

    Type
    Collection
    ...William Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in...

  19. Institute for Protein Innovation

    Type
    Collection
    ... other difficult-to-target antigens that are intractable via animal immunization. IPI is a nonprofit research...

  20. Pouring LB Agar Plates

    Type
    Protocol
    ... g pre-mixed powder consisting of: 5.0 g yeast extract 10.0 g peptone from casein 10.0 g sodium chloride.../mL 50 µg/mL Spectinomycin 50 mg/mL 50 µg/mL Tetracycline 10 mg/mL 10 µg/mL Notes: Unless otherwise indicated...
Showing: 41 - 60 of 517 results