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  4. The MTA at Addgene

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    Blog Post
    ...often get positive feedback on “our” MTAs, which sometimes makes people think our MTAs are different than... research use only to industry-based scientists. Some organizations choose to share their plasmids under...

  5. Addgene Depositors Get More Citations

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    Blog Post
    ...journals as those that did make deposits to Addgene.  Insomuch as different journals represent different research...using these weighted numbers. Note: this does drop some journals from our dataset, since not all journals...

  7. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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    Blog Post
    ...Our recent paper (Richardson et al 2016) revealed some of the biophysical parameters that can influence...genetic diseases in which sequence replacement holds some kind of fitness advantage but error-prone repair...

  8. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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    Blog Post
    ...BY fromGroell et.al, 2018.   3D models are somewhat of a middle ground between 2D assays and in vivo...of the adverse drug reaction. This system would somewhat act like a 2-step verification. All approaches...

  9. Components of CRISPR/Cas9

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    Blog Post
    ...' NAAAAC  The synthetic guide RNA or gRNA (sometimes sgRNA) In the native Type II CRISPR/Cas system...sequence) to modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes...

  10. Addgene Welcomes John Doench to the Advisory Board

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    Blog Post
    ...assignment that was open ended... it was actually something bordering on research, rather than 'here’s the..., sharing ethic that Addgene has behind it is something that very much resonates with me.” About Addgene...

  11. Plasmids 101: Mammalian Vectors

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    Blog Post
    ...a process called transfection. Transfection is somewhat comparable to bacterial transformation (the introduction...large-T antigen (293E or 293T cells), allow for episomal amplification of plasmids containing the viral...

  14. Antibodies 101: Isotypes

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    Blog Post
    ...constant domains, and the hinge region length. Now for some essential isotype nomenclature. Antibody heavy chain... the lower-case Greek letters μ, δ, γ, ε, and α. Some isotypes have different versions or subclasses that...

  15. GPCRs: How Do They Work and How Do We Study Them?

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    Blog Post
    ...activate many GPCRs are still unknown. To identify some of these unknown ligands and their GPCR binding ...off-target effects thus scientists then modified some GPCRs further to respond to specific, pharmacologically-inert...

  16. A Quick Guide to a Career in Software Product Management

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    Blog Post
    ...Technologies. If the first thing you hear when someone says they’re a software product manager is “project...sequencing (NGS), and high-throughput screening. While some developers had science backgrounds, most did not...

  17. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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    Blog Post
    ...Find plasmids for blue-white screening at Addgene! Some bacterial strains contain lacZ in their genome, ...sequencing primers in our Molecular Biology Reference. Sometimes common  primers can't provide all of the information...

  19. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ...adjusted voltage and compensated your colors. Here are some good practice tips on adjusting voltage: During ...would be shifted to the right in comparison, say somewhere between a 104–105 signal intensity. If your samples...

  20. Antibodies 101: Flow Cytometry Controls

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    Blog Post
    ... gating your target population (b). There is some debate in the literature as to when you need isotype...them to gate our populations, but others do not. Some journals require isotype controls, while others ...
Showing: 581 - 600 of 861 results