We narrowed to 869 results for: SOM

...realized that certain stains were retained only in some species of bacteria. The stain colored one type,...medicine and more successful experiments. It’s something that all scientists should understand when stepping...

...that CRISPR/Cas9 could be used to edit several autosomal-dominant mutations that have been connected to...disease in the near future,” Li says, though there are some things to sort out first. The potential for off-...

...BY fromGroell et.al, 2018.   3D models are somewhat of a middle ground between 2D assays and in vivo...of the adverse drug reaction. This system would somewhat act like a 2-step verification. All approaches...

...often get positive feedback on “our” MTAs, which sometimes makes people think our MTAs are different than... research use only to industry-based scientists. Some organizations choose to share their plasmids under...

...contributions.   Our volunteers are extremely diverse. Some of them are undergraduates or PhD students who are...researcher makes a small but crucial contribution to someone else’s project. We think that these collaborations...

...assignment that was open ended... it was actually something bordering on research, rather than 'here’s the..., sharing ethic that Addgene has behind it is something that very much resonates with me.” About Addgene...

...a process called transfection. Transfection is somewhat comparable to bacterial transformation (the introduction...large-T antigen (293E or 293T cells), allow for episomal amplification of plasmids containing the viral...

...we will focus on the most recent advances and how some of these variants may be appropriate for your specific...these off-target effects can be genome-wide and, in some cases, difficult to determine, the potential for...

...travels through a host organism, the expression of some genes can change depending on the location. Current...reporters have limited deep-tissue performance, and sometimes require an alternative such as a radioactive tracer...

...RNA sequence (in this case, norovirus RNA), the ribosome binding site is inaccessible. When the target ...however, it binds to the switch and exposes its ribosome binding site, allowing for the gene on the toehold...

...a lot of space and don’t tell you why you did something or what the result was, so try not to do this.... that I try to follow in my own research. Again, some of this may not apply or may not work for you. The...

...activate many GPCRs are still unknown. To identify some of these unknown ligands and their GPCR binding ...off-target effects thus scientists then modified some GPCRs further to respond to specific, pharmacologically-inert...

...Technologies. If the first thing you hear when someone says they’re a software product manager is “project...sequencing (NGS), and high-throughput screening. While some developers had science backgrounds, most did not...

...Find plasmids for blue-white screening at Addgene! Some bacterial strains contain lacZ in their genome, ...sequencing primers in our Molecular Biology Reference. Sometimes common  primers can't provide all of the information...

... should be included and what basic information someone new to antibodies might need to successfully start...separate from the author, and a second review from someone less familiar with the subject for clarity. Our...

...cells (Allen, et al., Frontiers in Genome Editing).  Some of the RNA stabilizing modifications listed above...applications like siRNA and antisense oligonucleotides. Some of these synthetic modifications, specifically ones...

...constant domains, and the hinge region length. Now for some essential isotype nomenclature. Antibody heavy chain... the lower-case Greek letters μ, δ, γ, ε, and α. Some isotypes have different versions or subclasses that...

...adjusted voltage and compensated your colors. Here are some good practice tips on adjusting voltage: During ...would be shifted to the right in comparison, say somewhere between a 104–105 signal intensity. If your samples...

... gating your target population (b). There is some debate in the literature as to when you need isotype...them to gate our populations, but others do not. Some journals require isotype controls, while others ...

...Bioluminescent reporters require a chemical substrate (and sometimes cofactors) to be provided and are generally dimmer...as their substrate is available. Table 1 compares some general advantages and disadvantages of each approach...