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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 658 results for: otos

Showing: 581 - 600 of 658 results

  1. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following...

  2. Viral Vector Guides and Plasmids

    Type
    Collection
    ...Viral Vector Packaging Service Addgene's Viral Protocols Addgene Blog Viral Vector Topics Page Viral Vectors...

  4. Antibody Plasmid Collection

    Type
    Collection
    ...synthetic nanobodies against delicate proteins. Nat Protoc. 2020 Apr 8. Markus Seeger Antibody Plasmids The...

  5. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Prime editing...

  7. Plant Plasmids and Resources

    Type
    Collection
    ... and gRNA backbone for use in rice. Includes a protocol (Link opens in a new window) for Targeted Gene...

  8. p53 Pathway

    Type
    Collection
    ...molecule Maspin Serpin family B member 5 MDM2 MDM2 proto-oncogene, E3 ubiquitin protein ligase MDMX p53 regulator...

  9. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base Edit ...

  10. Zebrafish Plasmid Collection

    Type
    Collection
    ...of gene function in zebrafish - Julian Lewis Lab PhOTO Zebrafish: A Transgenic Resource for In Vivo Lineage...

  11. CRISPR Plasmids - Plants

    Type
    Collection
    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following...

  12. Open Enzyme Collection

    Type
    Collection
    ...ampicillin. Find more information on the enzymes and protocols for expression in the Open Bioeconomy Lab's crowd-sourced...

  14. Deisseroth INTRSECT Collection

    Type
    Collection
    ...McElligott ZA, Holmes A, Heisler LK, Kash TL. 2016. Serotonin engages an anxiety and fear-promoting circuit ...

  15. Optogenetics Guide

    Type
    Guide
    ...reduces photocurrent amplitude 490 ChR/T159C T159C mutation; displays increased photocurrents 470 SFO/...bacterial photoreceptors to create protein systems controlled by light. These “photoswitchable” proteins...Kv) channels fused to LOV2 photoswitch. Illumination causes the photoswitch to unfold, lowering the toxin's...500 (off) PhoCl Photocleavable protein engineered from a green-to-red photoconvertible fluorescent protein... 400 UVR8 domains UVR8 plant photoreceptor protein that forms photolabile homodimers with slow reversal...responses, such as nonspecific cation influx vs. proton efflux. Researchers have used genetic engineering...neuron. Feature enhancements include: Increased photocurrent amplitude Examples: ChR2(H134R), C1V1(t/t), ...

  16. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...viral titer. For more detailed protocols, see Addgene’s viral vector protocols . Production Using Packaging...information in Addgene’s stable cell line protocol . Addgene’s protocol is for lentiviral vectors, but a similar...Addgene protocols Generating Stable Cell Lines Using Lentiviral Vectors Addgene's Viral Protocols References...enhancers. Many times, integration sites are found in proto-oncogenes. This can lead to activation of the oncogene... offer the benefit of having well-established protocols for fast and efficient generation of large-scale...carry a slightly elevated risk of integrating into proto-oncogenes. As with most experiments, infection risks...., Nikoniuk, A., Macmorland, W., Horlock, C., Matsumoto, S., Williams, S., Smith, K., Price, J., Srivastava...

  17. CRISPR Guide

    Type
    Guide
    ...edition) CRISPR software and depositor protocols Video protocol for genomic deletions in mammalian cell...target is present immediately adjacent to a P rotospacer A djacent M otif (PAM) The PAM sequence (NGG)...can increase the risk of off-target cutting or cytotoxicity, but naturally-occurring Acr (Anti-CRISPR) proteins...fusing the anti-CRISPR protein AcrIIA4 with the photosensitive LOV2 domain . Several labs have also controlled...before use (Figure 8C), following the amplification protocol specified by the depositing lab. After amplification...The translated codons that make up a gene PAM P rotospacer A djacent M otif; Sequence adjacent to the target...engineering using the CRISPR-Cas9 system. Nature Protocols , 8 (11), 2281–2308. PMID: 24157548 Ran, F. A....

  18. Educational Resources

    Type
    Guide
    ...including protocols, tips on using Addgene’s materials, and career advice. Playlists include: Protocols Careers... Molecular Cloning Techniques Protocols Find written and video protocols for a wide range of applications...videos, and protocols Educational... Educational Resources From eBooks to videos and blog posts to protocols, our educational...

  19. Lentiviral Vector Guide

    Type
    Guide
    ...transgene. For more detailed protocols, see Addgene’s viral vector protocols . Figure 5: Overview of lentiviral...Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols Lentiviral Vector Production...vectors can be found in Addgene’s stable cell line protocol . Browse Addgene's collection of in-stock lentiviral...Lines Using Lentiviral Vectors Addgene's Viral Protocols References Cronin, J., Zhang, X., & Reiser, J....mapping of lentiviral integration sites. Nature Protocols , 14 (1), 153–170. https://doi.org/10.1038/s41596...

  20. Plan Your Experiment

    Type
    Guide
    ...review the protocol associated with the specific plasmid in question (see CRISPR protocols from Addgene...10.1038/s41587-020-0490-7 PMID: 29305085 Hashimoto, M., & Takemoto, T. (2015). Electroporation enables the...used as positive controls when working out a new protocol. Read more about how to design your gRNA . Synthesize...editing experiments in mammalian cells. Nature Protocols , 17 (11), 2431–2468. https://doi.org/10.1038/...
Showing: 581 - 600 of 658 results