We narrowed to 660 results for: otos

...download. CRISPR Resources : Browse depositor protocols, find software for gRNA design and deep sequencing...

...reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content...

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... YouTube Videos Addgene's Biosafety Resources Protocols General Transfection Bert Vogelstein’s lab practical...

...scientists to best utilize your plasmids (these can be protocols, GenBank files, etc). We can accept sequence files...

...Viral Vector Packaging Service Addgene's Viral Protocols Addgene Blog Viral Vector Topics Page Viral Vectors...

...synthetic nanobodies against delicate proteins. Nat Protoc. 2020 Apr 8. Markus Seeger Antibody Plasmids The...

...reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content...

...molecule Maspin Serpin family B member 5 MDM2 MDM2 proto-oncogene, E3 ubiquitin protein ligase MDMX p53 regulator...

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...integration can occur. Okita et al describes a protocol for generating iPSCs by plasmid transfection where...

...of gene function in zebrafish - Julian Lewis Lab PhOTO Zebrafish: A Transgenic Resource for In Vivo Lineage...

...ampicillin. Find more information on the enzymes and protocols for expression in the Open Bioeconomy Lab's crowd-sourced...

...expressing sgRNA and Cas9 in rice . Includes a protocol (Link opens in a new window) for targeted gene...

...McElligott ZA, Holmes A, Heisler LK, Kash TL. 2016. Serotonin engages an anxiety and fear-promoting circuit ...

...reduces photocurrent amplitude 490 ChR/T159C T159C mutation; displays increased photocurrents 470 SFO/...bacterial photoreceptors to create protein systems controlled by light. These "photoswitchable" proteins...Kv) channels fused to LOV2 photoswitch. Illumination causes the photoswitch to unfold, lowering the toxin's...500 (off) PhoCl Photocleavable protein engineered from a green-to-red photoconvertible fluorescent protein... 400 UVR8 domains UVR8 plant photoreceptor protein that forms photolabile homodimers with slow reversal...responses, such as nonspecific cation influx vs. proton efflux. Researchers have used genetic engineering...neuron. Feature enhancements include: Increased photocurrent amplitude Examples: ChR2(H134R), C1V1(t/t), ...

...viral titer. For more detailed protocols, see Addgene’s viral vector protocols . Production Using Packaging...information in Addgene’s stable cell line protocol . Addgene’s protocol is for lentiviral vectors, but a similar...Addgene protocols Generating Stable Cell Lines Using Lentiviral Vectors Addgene's Viral Protocols References...enhancers. Many times, integration sites are found in proto-oncogenes. This can lead to activation of the oncogene... offer the benefit of having well-established protocols for fast and efficient generation of large-scale...carry a slightly elevated risk of integrating into proto-oncogenes. As with most experiments, infection risks...., Nikoniuk, A., Macmorland, W., Horlock, C., Matsumoto, S., Williams, S., Smith, K., Price, J., Srivastava...

...edition) CRISPR software and depositor protocols Video protocol for genomic deletions in mammalian cell...target is present immediately adjacent to a P rotospacer A djacent M otif (PAM) The PAM sequence (NGG)...can increase the risk of off-target cutting or cytotoxicity, but naturally-occurring Acr (Anti-CRISPR) proteins...fusing the anti-CRISPR protein AcrIIA4 with the photosensitive LOV2 domain . Several labs have also controlled...before use (Figure 8C), following the amplification protocol specified by the depositing lab. After amplification...The translated codons that make up a gene PAM P rotospacer A djacent M otif; Sequence adjacent to the target...engineering using the CRISPR-Cas9 system. Nature Protocols , 8 (11), 2281–2308. PMID: 24157548 Ran, F. A....

...including protocols, tips on using Addgene’s materials, and career advice. Playlists include: Protocols Careers... Molecular Cloning Techniques Protocols Find written and video protocols for a wide range of applications...videos, and protocols Educational... Educational Resources From eBooks to videos and blog posts to protocols, our educational...

...transgene. For more detailed protocols, see Addgene’s viral vector protocols . Figure 5: Overview of lentiviral...Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols Lentiviral Vector Production...vectors can be found in Addgene’s stable cell line protocol . Browse Addgene's collection of in-stock lentiviral...Lines Using Lentiviral Vectors Addgene's Viral Protocols References Cronin, J., Zhang, X., & Reiser, J....mapping of lentiviral integration sites. Nature Protocols , 14 (1), 153–170. https://doi.org/10.1038/s41596...