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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 610 results for: sam

Showing: 581 - 600 of 610 results

  1. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...before you place the sample at -80°C. Frozen tubes are hard to write on and samples stored for long periods...glycerol. You can prepare the glycerol stock the same time you prepare your plasmid DNA. In the morning...

  2. Video Library

    Type
    Protocol
    ...Pipetting Technique Learn how to pipette multiple samples at once using a multichannel pipette and avoid ...bands out of the agarose gel and purify the DNA samples. This is a commonly used technique for molecular... Your guide to the MTA process when requesting samples from Addgene Technology Transfer How to Order A...

  3. Immunocytochemistry

    Type
    Protocol
    ...optimal fixation method will vary depending on the sample type and the protein of interest. You may need ...expected and specific, include a positive control sample that you know expresses the protein, such as cells...the protein of interest, and a negative control sample such as cells that do not express the protein of...

  4. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...of DNA Samples Add an equal volume of TE-saturated phenol-chloroform to the aqueous DNA sample. Pro-Tip...remaining contaminant proteins and RNase A from the DNA sample. When phenol is mixed with the aqueous solution...

  5. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...absorbed. The spreading of cells can be done in the same way as the antibiotic, using either a bent micropipette...over-agar for selection of transformed E. coli . Sample Data Selection of E.coli on LB-agar using different...

  6. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...two different shRNAs that target the same gene can produce the same phenotype will alleviate concerns about...boiling water. If using a PCR machine, incubate the sample at 70°C for 10 minutes then slowly cool to room...

  8. Colony Formation Titering Assay

    Type
    Protocol
    ...protocol outlines the seeding of the cells at the same time as the virus-mediated transduction. For some... titer, take the average of multiple dilutions. Sample Data Figure 1: A549 cells were transduced with ...

  9. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...complete + 10 µg/mL polybrene. Note, this is just a sample of possible dilutions. You may want to try higher...monoclonal lines from the early polyclonal populations. Sample Data Figure 1: Generation of monoclonal cell lines...

  10. Antibody Guide

    Type
    Guide
    ...application Collect and lyse samples to make proteins available. Tissue samples may need additional processing...culture samples to extract proteins. Homogenize (tissues only) and lyse. Cross-link the samples with formaldehyde...isotype. Overview of application Fix your samples. Prepare your samples using permeabilization, antigen retrieval...Blank controls in the form of sample buffer must also be run. Unique samples (non-biological replicates)...cultured B cells, where each culture should produce the same antibody clone, or from plasmid-based expression...factors, binding partners, location in the cell, and sample processing can hide or reveal epitopes on an antigen...contain antibodies targeting various epitopes on the same antigen. Polyclonal antibodies are often more environmentally...

  11. CRISPR Guide

    Type
    Guide
    ...protein, instead of a two-component system (Figure 9C) SAM activators - co-expression of dCas9-VP64 with a modified... ensures that all gRNAs will be expressed in the same cell. This is called multiplexing , and increases...deletions or inversions with two gRNA sites on the same chromosome. Most multiplex systems enable researchers...sequence ~1 kb shorter than SpCas9 while retaining the same basic function. Its size allows it to be efficiently...from Neisseria meningitidis ), which are about the same size as SaCas9, with higher fidelity than SpCas9... types of base edits: Base transitions - edit to same classification of nucleotide base (purine-to-purine...an enzyme evolved to excise inosine to access the same repair pathway and convert A to any other base. ...

  12. Protocol - How to Design Primers

    Type
    Protocol
    ...primer is used to amplify a eukaryotic genomic DNA sample. However, a primer should not be too long (> 30...

  14. Ligation Independent Cloning

    Type
    Protocol
    ...have different leader sequences, but operate on the same principle (homologous to the first G on 3'-5' strand...

  16. Fluorescence Titering Assay

    Type
    Protocol
    ... titer, take the average of multiple dilutions. Sample Data Figure 1: 293T cells were transduced with ...

  17. General Transfection

    Type
    Protocol
    ...24–48 h before checking for protein expression. Sample Data Legend: Lenti-X 293T cells were transfected...

  18. Lentivirus Production

    Type
    Protocol
    ... °C as soon as possible to avoid loss of titer. Sample Data Figure 1: 293T cells were transfected with...

  20. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Both lentiviruses and gamma-retroviruses use the same packaging genes. However, they are different viruses...with gamma-retroviral vectors is essentially the same as lentiviral vectors. Many gamma-retroviral vectors...in cis refers to genetic elements located in the same plasmid as the gene of interest. in trans In the...
Showing: 581 - 600 of 610 results