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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 620 results for: PROC

Showing: 601 - 620 of 620 results

  1. Lentivirus ddPCR Titration

    Type
    Protocol
    ...15 mL DMEM Complete 3 µL of 250 U/µL benzonase Procedure Transducing Cells Thaw the virus on ice. Prepare...Seal’ button. After the plate has been sealed, proceed to thermocycling. Thermal Cycling Run the following...

  2. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...found at the Broad Institute's TRC shRNA Design Process . To minimize degradation of off-target mRNAs, ...institution-specific instructions. You must follow safety procedures and work in an environment (e.g. BL2+) suitable...

  5. Weighing Reagents Protocol

    Type
    Protocol
    ....) Reagents Material that will be weighed out Procedure The balance in the photo above has a capacity ...

  6. Water Bath Protocol

    Type
    Protocol
    ... bath weights and floats Reagents None needed Procedure Ensure that the water bath and water bath cover...

  9. Fluorescence Titering Assay

    Type
    Protocol
    ... supernatants to multiple freeze-thaw cycles. Procedure Seed 75,000 cells into each well of a 6-well dish...

  12. General Transfection

    Type
    Protocol
    ...the best ratio of mass DNA:mass PEI determined. Procedure Seed HEK293T cells at 3.8x10 6 cells per plate...

  13. Gibson Assembly Protocol

    Type
    Protocol
    ...reaction. Can combine many DNA fragments at once. Procedure Design your plasmid and order primers (see figure...

  14. Immunocytochemistry

    Type
    Protocol
    ...stock solution into 5 mL PBS. Protect from light. Procedure Section 1: Seeding cells Place a sterile poly-...

  15. Colony Formation Titering Assay

    Type
    Protocol
    ...transduction, and not during the cell seeding step. Procedure Before beginning a colony formation assay, the...

  17. Pipetting Protocol

    Type
    Protocol
    ...the P2 this represents the third decimal place. Procedure How to Pipette Now that you know the different...

  18. Western Blot

    Type
    Protocol
    ...antibody for primary antibodies raised in a mouse. Procedure Section 1: Lyse cells Centrifuge 5 x 10 6 cells...

  20. Kit Free RNA Extraction

    Type
    Protocol
    ...with the volatile reagents in the list above. Procedure Option #1 - Solution D Protocol Before starting...
Showing: 601 - 620 of 620 results