We narrowed to 630 results for: PROC

...polyethylenimine (PEI) transfection protocol. This procedure can be modified for alternative packaging cell...that are below passage 15 for viral production. Procedure Seed 293T packaging cells at 3.8×10 6 cells per...

...filter. Aliquot and freeze upright at -20 °C. Procedure Section 1: Seeding cells The day prior to transfecting...between 1.5–2 x 10 6 cells/mL with >95% viability to proceed with transfection. Transfect the flask containing...

...of Buffer B and 2 mL of 5 M NaCl to 43 mL PBS Procedure Preparation and loading of the iodixanol gradient...0.001% in PBS: 45 mL PBS + 5 ml B + 200 mM NaCl Procedure Cover the filter membrane with 15 mL of 0.1% Poloxamer...

...subcloning), including design and experimental procedures....will save you time in future steps. Experimental Procedure Digest your DNA Set up restriction digests for...

... cooling (denaturing and annealing) cycle. The process became automated with the discovery of a heat resistant...Polymerase (5 units/μL) PCR Machine Agarose Gel Procedure Primer Design and PCR Design Primers. See our ...

... be subjected to multiple freeze-thaw cycles. Procedure Before beginning, determine the optimal dose of...antibiotic. This is the beginning of the selection process, which will begin the selection of a stable cell...

...0.15 μL 15 μL Nuclease Free Water 4.7 μL 470 μL Procedure Prepare a plasmid stock of 2 x 10 9 molecules/...contaminating plasmid DNA carried over from the production process (DNase does not penetrate the virion). 5 μL sample...

... mL of deionized water. Gently invert to mix. Procedure Section 1: SDS-PAGE Add 5 µL of 4X sample buffer... indicates that the samples may not have been processed correctly in the cell (e.g., failure to cleave...

...for different cell types. Before starting this procedure, be sure that the cell pool has been sufficiently...well plate gave rise to 15–20 monoclonal lines. Procedure Generating Conditioned Medium (optional) Seed ...

...Poloxamer 188 4475 µL Molecular Biology Grade Water Procedure Preparation Before handling any viruses, get materials...Seal’ button. After the plate has been sealed, proceed to thermocycling. Thermal Cycling Run the following...

...15 mL DMEM Complete 3 µL of 250 U/µL benzonase Procedure Transducing Cells Thaw the virus on ice. Prepare...Seal’ button. After the plate has been sealed, proceed to thermocycling. Thermal Cycling Run the following...

...found at the Broad Institute's TRC shRNA Design Process . To minimize degradation of off-target mRNAs, ...institution-specific instructions. You must follow safety procedures and work in an environment (e.g. BL2+) suitable...

...learn how to create bacterial glycerol stocks. Procedure Follow the steps for Inoculating an Overnight ...

...resistance gene (or other antibiotic resistance) Procedure Day 1 Prepare carbenicillin to a concentration...

....) Reagents Material that will be weighed out Procedure The balance in the photo above has a capacity ...

... bath weights and floats Reagents None needed Procedure Ensure that the water bath and water bath cover...

...the building blocks for many more complicated procedures. Name Description (Link opens in a new window...

... supernatants to multiple freeze-thaw cycles. Procedure Seed 75,000 cells into each well of a 6-well dish...

...For long term storage of the bacteria, you can proceed with creating a glycerol stock . You can now isolate...

...reaction. Can combine many DNA fragments at once. Procedure Design your plasmid and order primers (see figure...