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We narrowed to 957 results for: REP

Showing: 601 - 620 of 957 results

  1. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...sequencing. The sequencing results covered TFs representing 92% of the MORF library, with an average of ...generate, in part because of difficulty with replicating the specific dysregulation in the disease. For...Documentation of the network that initiates biological reprogramming is a valuable reference if you are looking to...

  2. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ... when compared to the conventional AAVs (AAV preparations were always normalized to their genomic titers...retina upon intravitreal injection." Scientific reports 7 (2017): 45329. PubMed PMID: 28361998. PubMed ...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

  3. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...might like to store about FPs than is currently represented at fpvis (complete spectral information, accession...rapidly growing, with your help!), I believe this represents one of the largest available online collections...analysis tools, and a better way to store and represent within-study photobleaching comparisons.  We very...

  4. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...plasmid and viral vectors. In applications when repetitive tRNA sequences must be avoided, different tRNAs...).   Fig. 2: Comparison of previous representative dual-gRNA architectures with DAP array ...Multiplex expression of gRNAs Four Base Editing Reporters to Monitor and Enrich Editing in Real Time Prime...

  6. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...Here are a few things to consider when you're preparing your resume for a non-academic position: What ...services, but some of the general knowledge about preparing a good resume, writing a concise cover letter,..., but the earlier you ask yourself, the better prepared you are for your next step. Here are some great...

  7. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...enough to ensure the virus can replicate and spread. Instead, viruses replicate by hijacking the host’s system...people in nature, extra safety measures, like replication incompetent viral particles, are required. We...

  8. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    ...through many rounds of mutations, selection, and replications per day.  The PACE and PANCE systems use M13...uninfected host cell culture). This dilution step represents the major difference between PACE and PANCE: ...that change C to T, G to A, A to G, and T to C, represent about two-thirds of known pathogenic point mutations...

  9. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...our strategy using the pMBP-LbCas12a and pC013-Twinstrep-SUMO-huLwCas13a plasmids from Addgene, which would...a seminar on “Tools to increase scientific reproducibility” by Benoit Giquel, an outreach scientist from...The talk highlighted the importance of reagent repositories and the myriad facilities offered by them which...

  10. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...each with subcategories such as plasmids and viral preps for catalog, blog posts and protocols for educational...and vector type, while a search scoped to viral preps shows filters for promoter and serotype, among others...filter value. For example, there is only one viral prep filter for promoter “Syn”, that, when selected, ...

  11. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...increasing the standard mutation rate seen during DNA replication by 120-fold over background. Due to its modular...bacteria resistant to both spectinomycin and streptomycin, but could also be used for studying epistatic...generate specific mutations in your plasmids Use REPLACER Mutagenesis to mutate your plasmid Resources ...

  12. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...persuading an employer that your past work has prepared you to contribute at a high level to their team...audience your primary focus When writing a paper or preparing a presentation, it's easy to forget that you have...form? Training in science communication should prepare you to ask and answer these questions for a diverse...

  13. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    ... properties. The phagemids are packaged into replication-incompetent phage particles with assistance from...and AAV plasmids as well as ready-to-use viral preparations at Addgene. For more information on viral vectors... found on an episome, a piece of DNA that can replicate on its own or be integrated within a bacterial...

  14. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...will see two bands: one representing your vector backbone and the other representing the insert. If the plasmid...using lysed bacterial colonies, without the need to prep your plasmid. In this case, you’d be using colony...

  15. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...all viral genes except ITRs (inverted terminal repeats – the sequences that flank the deliverable DNA)...transduced gene is episomal and does not divide and replicate in the same way the host DNA does. Because of ... since you don’t have to wait for the host to replicate the DNA before expression. Now that you’ve got...

  17. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...contains ccdB. A successful insertion will completely replace ccdB with the investigator’s insert of interest...ccdB SurvivalTM, one can efficiently propagate and prep plasmids containing the ccdB gene, which can then... strains could possibly be used to propagate and prep your ccdB-containing empty backbones but should ...

  18. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ... an ERK kinase translocation reporter with a mRuby2 fluorescent reporter (ERK KTR-mRuby2). The ERK KTR...Activity with GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable fluorescent proteins...

  19. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...fluorescent biosensor. Scientific Reports,5(1). doi:10.1038/srep12397. PubMed PMID: 26215030. PubMed Central...2008). Rosella: A fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in...

  20. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...community. This is no small task, however, at a repository that consistently receives around 200 new DNA... over 2000 primers. Two 96-well PCR plates are prepared with the primer stocks for forward and reverse...primers that bind within or must read through repetitive regions (such as LTRs or ITRs), hairpins, or ...
Showing: 601 - 620 of 957 results