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  1. Antibodies 101: Isotypes

    Type
    Blog Post
    ...constant domains, and the hinge region length. Now for some essential isotype nomenclature. Antibody heavy chain... the lower-case Greek letters μ, δ, γ, ε, and α. Some isotypes have different versions or subclasses that...

  2. GPCRs: How Do They Work and How Do We Study Them?

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    Blog Post
    ...activate many GPCRs are still unknown. To identify some of these unknown ligands and their GPCR binding ...off-target effects thus scientists then modified some GPCRs further to respond to specific, pharmacologically-inert...

  3. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...Technologies. If the first thing you hear when someone says they’re a software product manager is “project...sequencing (NGS), and high-throughput screening. While some developers had science backgrounds, most did not...

  4. How to Keep a Lab Notebook for Bioinformatic Analyses

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    Blog Post
    ...a lot of space and don’t tell you why you did something or what the result was, so try not to do this.... that I try to follow in my own research. Again, some of this may not apply or may not work for you. The...

  6. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...like a lot of specialized science, these tools sometimes have limited distribution. We hope that will change...navigate when mapping out a new project. We’ve shared some of the concerns that Revolution Bio had to address...

  8. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ... a homologous template provided by the sister chromosome. As cells take those two ends and put them back...there is a possibility of introducing a mutation somewhere else in the genome. It remains to be seen which...

  10. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...light. Any fluorescent molecule will photobleach at some point. For live-cell imaging, it is desirable to...different ways. We describe a number of issues with some of the experimental designs used to make these measurements...

  11. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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    Blog Post
    ...strains ultimately perform the same function, there is some evidence that ccdB Survival™ can be more difficult...plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes.” J Mol Biol. 1992 Aug 5;226(3)...

  12. Transferable Skills Guide: Cross-team Communication

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    Blog Post
    ... a month or so of meetings, I began to notice something: even though our scientists and engineers were...versa), then you have almost certainly experienced some of the hurdles I needed to overcome.  I eventually...

  14. Plasmids 101: The protein expression toolbox

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    Blog Post
    ...protein expression control tools as well as explore some new innovations. Whether you want to turn things...more difficult than a simple knock out. Looking for some permanent knock out plasmids? Check out our Cre ...

  15. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post
    ...cells (Allen, et al., Frontiers in Genome Editing).  Some of the RNA stabilizing modifications listed above...applications like siRNA and antisense oligonucleotides. Some of these synthetic modifications, specifically ones...

  16. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...by offering advice on areas where you might need some help. Today in our transferable skills guide: Problem-Solving... question you have about the world. You might do some background research to better understand it and ...

  18. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...Gate cloning into a modular cloning strategy. Sometimes referred to as MoClo, this strategy uses the Type...Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. ...
Showing: 601 - 620 of 861 results