We narrowed to 869 results for: SOM

... difficult to replicate. Unclear methods Until someone attempts to replicate the results of another’s ...original research finding we can be more certain that some important, previously unknown variable actually ...

...a lot of space and don’t tell you why you did something or what the result was, so try not to do this.... that I try to follow in my own research. Again, some of this may not apply or may not work for you. The...

...abilities to turn genes on and off,” he said. “Some are strong and some more moderate; there is an allelic series...

...like a lot of specialized science, these tools sometimes have limited distribution. We hope that will change...navigate when mapping out a new project. We’ve shared some of the concerns that Revolution Bio had to address...

...explained that the data must be reproducible by someone else. Second, "an entrepreneur must be OK with ...importantly, “the company does not become real until someone takes the risk full-time and abandons all safety...

... a homologous template provided by the sister chromosome. As cells take those two ends and put them back...there is a possibility of introducing a mutation somewhere else in the genome. It remains to be seen which...

...backbone, but they express or target different genes. Some libraries cover the majority of the genome, while...the depositor’s protocol for the best results. For some libraries, plasmid DNA can be delivered directly...Vector resources . Check out our blog posts featuring some of our most popular libraries! Genome-wide Screening...

...order aggregates. This property can be tolerated in some applications (e.g. labeling of cells or tracking...uploading it as a preprint, or documenting it in some other way. Besides the data, new plasmids and/or...

...light. Any fluorescent molecule will photobleach at some point. For live-cell imaging, it is desirable to...different ways. We describe a number of issues with some of the experimental designs used to make these measurements...

...strains ultimately perform the same function, there is some evidence that ccdB Survival™ can be more difficult...plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes.” J Mol Biol. 1992 Aug 5;226(3)...

... a month or so of meetings, I began to notice something: even though our scientists and engineers were...versa), then you have almost certainly experienced some of the hurdles I needed to overcome.  I eventually...

...individuals with similar ideas to actually do something about it. Building a diverse team with different... industry partners, not the other way around. Sometimes, due to misconceptions and stereotypes, solving...

...by offering advice on areas where you might need some help. Today in our transferable skills guide: Problem-Solving... question you have about the world. You might do some background research to better understand it and ...

...components. Nearly all of us played with LEGO as a child (some of us still do), so we are used to the idea of general...test out lots of combinations, to end up finding something surprisingly useful. To apply these principles...

...protein expression control tools as well as explore some new innovations. Whether you want to turn things...more difficult than a simple knock out. Looking for some permanent knock out plasmids? Check out our Cre ...

... these options can be cumbersome, can lower on-target efficiency, and sometimes even increase off-target...

...Gate cloning into a modular cloning strategy. Sometimes referred to as MoClo, this strategy uses the Type...Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. ...

...homo-oligomerization of a protein fused to CRY2, although some studies have shown that this clustering only happens...To overcome this issue, Shemesh et. al. created a soma-targeted derivative of the opsin CoChR, termied ...

...visual person. Why don’t you record a podcast or put some effort into making a video? Use your unique talent...annual meetings. 8) Training – Get a formal education Some people get into science communication by taking ...

...methods aren’t stealthy enough to gain access to some cells and tissues, such as human induced pluripotent...zygotes with CRISPR components. However, this sometimes requires intracellular injections into the cytoplasm...