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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 625 results for: Emb

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  1. Lentiviral Vector Guide

    Type
    Guide
    ... methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited...requires a production cell line, typically human embryonic kidney 293T (HEK293T) cells. Standard HEK293T ... infection risks occur with contact to mucous membranes or broken skin. Needle sticks and ripped gloves...

  2. Molecular Biology Reference

    Type
    Guide
    ...nucleotide is present at the end of each fragment and assembles that information into the DNA sequence. Sanger...reactions on different pieces of DNA to be able to assemble the whole puzzle. That’s where Next Generation...sequenced templates are then aligned to each other to assemble the entire sequence or puzzle. It is important...

  3. Plan Your Experiment

    Type
    Guide
    ...read our three tips to improve HDR efficiency . Remember that HDR also requires donor DNA to serve as template...can transfect at high efficiency, such as human embryonic kidney 293 (HEK293) cells. For more difficult ...

  4. CRISPR Guide

    Type
    Guide
    ...against these foreign nucleic acids. Acr family members inhibit CRISPR by a variety of mechanisms, including...Mediated Genetic Screening in Mice with Haploid Embryonic Stem Cells Carrying a Guide RNA Library. Cell ...

  5. Chemogenetics Guide

    Type
    Guide
    ...cell surface receptors, GPCRs are seven-pass transmembrane proteins that bind a specific ligand. Ligand...
Showing: 621 - 625 of 625 results