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We narrowed to 1,015 results for: LEA

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Showing: 931 - 960 of 1015 results

  3. Antibody Guide

    Type
    Guide
    ...code inserted into the plasmid. This produces the least amount of variation between antibodies, allows for... induced to express antibodies. Antibodies are released into the growth medium and can either be used ...fragments - Fab fragments are created by enzymatically cleaving the variable regions of an antibody (each Fab ...they should be stored at -20 °C in aliquots of at least 10 µL. Antibodies cannot tolerate freeze-thaw cycles...single cells. Includes: Flow cytometry Read on to learn more about the applications common to each category...should be run with your protein of interest and at least one ubiquitously expressed protein such as GAPDH...protein:DNA complexes. After capture, the DNA is released from the complex and can be sequenced and amplified...

  14. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...the goal of the experiment Mammalian cells At least one of the following: to stably transfect the vector...

  19. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Read our adeno-associated viral vector guide to learn about AAV vector components, production and common...recombination between the transfected plasmids could lead to the production of replication-competent viruses...element necessary for RABV trans-synaptic transport. Learn more about these and other initiatives in our blog... packaged in the relatively small rAAV capsids. Learn more about optogenetics by watching our Optogenetics...stimulates the expression of transgenes via increased nuclear export. Poly(A) signal in cis The polyadenylation...polyadenylation or poly(A) signal aids in the nuclear export of RNA and RNA translation, and promotes RNA transcript...like viruses or bacteria, within a host organism, leading to disease. Insertional mutagenesis Random insertion...

  20. Five Popular Model Organisms

    Type
    Blog Post
    ...primarily characterized by protein misfolding that leads to a build-up of protein aggregates termed “lewy...

  22. Lentiviral Vector Guide

    Type
    Guide
    ...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and...lentiviruses has a long incubation period that eventually leads to serious disease and occurs in many species. Some...comparison of second- and third-generation systems, please refer to Table 1. Figure 4: Third-generation lentiviral...HEK293T cells will produce the viral vectors and release them into the supernatant. After an initial media...stimulates the expression of transgenes via increased nuclear export. RRE in cis Rev Response Element; the sequence...and partially spliced transcripts to facilitate nuclear export. Provided by a separate plasmid from gag...like viruses or bacteria, within a host organism, leading to disease. Lentivirus A retrovirus from the Retroviridae...

  23. Modular Cloning Guide

    Type
    Guide
    ...Learn about modular cloning (MoClo) and find MoClo kits deposited with Addgene, including the MoClo Toolkit...BpiI/BbsI to cut outside their recognition site, leaving DNA fragments with an overhang of 4 bp. If each...integrate up to three transcription units in the oleaginous yeast Y. lipolytica . EXPRESSYALI Yeast Expression...expressing multiple gRNAs (up to seven) with a Cas9 nuclease, nickase, dCas9, or dCas9-FokI fusion for use ...Vladimir Nekrasov 95 plasmids including CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for..., TALEN Feng Zhang Plasmids for building TALE nucleases (TALENs) and transcription factors (TALE-TFs) ...

  24. Optogenetics Guide

    Type
    Guide
    ...biomolecular processes with light. Read this guide to learn more, or explore Addgene's Optogenetics Plasmid ...relevant variants. This list is not exhaustive — please browse our Optogenetics Plasmid Collection to find...of a protein of interest. A genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion...voltage (LOV) domain of FKF1 LINUS light-inducible nuclear localization signal based on the LOV2 domain of...phototropin 1 LOVETRAP reversibly sequester and release proteins from cellular membranes using light. light-induced...protein in the light ~400 (on)/~500 (off) PhoCl Photocleavable protein engineered from a green-to-red photoconvertible...

  25. Molecular Biology Reference

    Type
    Guide
    ...Learn about the basics of molecular biology, including molecular genetics, plasmids, sequencing, and ...cell division, and each daughter cell receives at least one copy of the plasmid. By the 1970s, the combined... ( ori ), an antibiotic resistance gene, and at least one unique restriction enzyme recognition site (...CRISPR technology. CRISPR is composed of a DNA endonuclease and guide RNAs that target specific locations...genes, marking the birth of molecular genetics. To learn more about different types of cloning methods, check...of its sequence to design a effective primer. To learn more about the sequencing technologies Addgene uses...

  30. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Read our gamma-retroviral guide to learn about gamma-retroviral components, retroviral production, and.... The HEK293T cells will produce the virus and release it into the supernatant. After an initial media...integration sites are found in proto-oncogenes. This can lead to activation of the oncogene and possible development...stimulates the expression of transgenes via increased nuclear export. Psi (Ѱ) in cis RNA packaging signal; recognized...like viruses or bacteria, within a host organism, leading to disease. Provirus The genetic material (cDNA...
Showing: 931 - 960 of 1015 results