...be packaged in a variety of capsids. To date, at least 12 different capsids, or serotypes, have been identified...

...preferences. It sounds complicated but has a pretty low learning curve! Restraint: scruffing Location: throat Gauge...

... cells, and its lifecycle ends in cell lysis, releasing the virus to the supernatant. The virus will reach...

...the fluorescent signature upon antibody binding, leading to discrepancies during unmixing. Additionally,...

...compensation values later. This can be helpful as you're learning! Ashlyn has a research background in CD8+ T cells...

...science happens in a vacuum!  Munro and Pelham learned of these new antibodies and identified Myc1-9E10...

... have your parts. That was the motivation for releasing a large parts kit. Once you have so many parts...

...: An Inside Look at NGS Plasmid Quality Control Learn about our Snapgene-powered plasmid maps. Resources...

... their minds, but I think many of the students learned something. Based on the initial guidelines I received...

...recombination events to assemble the final plasmid. At least 5 fragments can be correctly assembled using IVA...

...quantified, and validated, it’s ready for use! You can learn more about common antibody applications in our Antibodies...

...gene does not fully suppress Zeocin-induced DNA cleavage in human cells. Mutagenesis, 20(2), 111–114. https...

...This limitation may be the both most obvious and least flexible one. Tissue expansion is not compatible...

...replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter...

...the Addgene Blog Read more Inside Addgene Posts! Learn all about Plasmids in our Plasmids 101 series! ...

...first disease-causing mutations in the MECP2 gene leading to Rett Syndrome (Amir et al., 1999). We now know...

... about plasmids in our Plasmids 101 blog series Learn more about trends in synthetic biology in Natural...

...the goal of the experiment Mammalian cells At least one of the following: to stably transfect the vector...

...Created with BioRender.com. Inosine excision leads to the first adenine transversion editors Uracil...

... chromosomal DNA ensure that the helicases are released from the DNA once the replication forks reach ...

.... It’s far better to verify via NGS now than to learn some of your plasmids didn’t amplify after your ...

...Physical and genetic studies with restriction endonucleases on the broad host-range plasmid RK2. Molecular...

...Learn about the types of plasmids used to produce AAV in the lab. Addgene’s AAV Guide will teach you ...genome editing. For more information on AAV-CRISPR, please see this blog post. Browse the article from Ran...particles produced during a lytic cycle are quickly released through cell lysis. Episomal stability enables...

...primarily characterized by protein misfolding that leads to a build-up of protein aggregates termed “lewy...

...integrin α4β1 required and conferred less force, leading cells to spread into symmetrical circles. While...

...firing through stimulating phospholipase C, which releases intracellular calcium stores. There are currently...For a discussion of alternative DREADD ligands, please see the text. Browse Addgene's collection of DREADD...(Fz) or chemical variations thereof (FFz, CFz). Learn more about optogenetic systems in our Optogenetics...

...Troiano, E. C., Moghadaszadeh, B., Peacker, B. L., Leacock, K. A., Horwitz, N., Beggs, A. H., Wagers, A. J...

...PMC4737176. Additional Resources on the Addgene Blog Learn New Plasmid Cloning Techniques Pushing the Limits...

... by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA. J Biotechnol 241: 136-146. PubMed...

...editing abilities, such as base editing, nicking, cleaving, etc. The possibilities really are endless: genetic...