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  2. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    .... Use a (Link opens in a new window) ligation calculator to easily quantify how much vector and insert...vector end. (Link opens in a new window) Ligation calculators are easily found on the web. Just enter the concentration...

  3. Colony Formation Titering Assay

    Type
    Protocol
    ...1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents...Swirl gently to mix and place the dish in the incubator. Incubate for 48–72 h. Pro-Tip Different cells...

  4. Using a Light Microscope Protocol

    Type
    Protocol
    ...research and are found in many different types of laboratories. These tools allow you to observe specimens ... in the future. Conclusion Like any important laboratory instrument, you should be sure to take care of...

  5. Pipetting Protocol

    Type
    Protocol
    ...liquid (think: 0.1 µL to 1 mL). When working in a laboratory, properly dispensing liquid ensures the accuracy...Reagents Liquid for pipetting Background Information Anatomy of a Pipette This front view photo of a P1000 pipette...

  6. Educational Resources

    Type
    Guide
    ... a wide range of topics in molecular biology, laboratory research, and career development. eBooks Download...

  7. DNA Quantification

    Type
    Protocol
    ... to protein (260/280) is generally used as an indicator of the purity of DNA samples. These days, many...

  8. Adenovirus Guide

    Type
    Guide
    ...information about using the AdEasy™ System? The laboratory of Bert Vogelstein has published their method...

  9. Retrovirus Guide

    Type
    Guide
    ...Woodchuck hepatitis virus post‐transcriptional regulatory element; sequence that stimulates the expression...

  10. Weighing Reagents Protocol

    Type
    Protocol
    ... reagents for a stock mixture is an essential laboratory technique, as imprecise measurements can affect...

  12. Fluorescence Titering Assay

    Type
    Protocol
    ...1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Inverted...

  13. General Transfection

    Type
    Protocol
    ...1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents...

  14. Gibson Assembly Protocol

    Type
    Protocol
    ...especially useful for introducing promoters, terminators, and other short sequences into the assembly ...

  15. Lentivirus Production

    Type
    Protocol
    ...1000 µL single channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container pH ...

  18. Western Blot

    Type
    Protocol
    ...the Top Stack to one side and discard the white separator. Keep the Bottom Stack in the plastic tray. Place...

  19. AAV ddPCR Titration

    Type
    Protocol
    ... Transfer the cartridge holder to the droplet generator. Close the lid and wait for the droplets to be...

  20. Lentivirus ddPCR Titration

    Type
    Protocol
    ... Transfer the cartridge holder to the droplet generator. Close the lid and wait for the droplets to be...
Showing: 621 - 640 of 641 results