We narrowed to 651 results for: EPO
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TypeCollection...these antibodies, IPI will be indicated as the Depositing Lab on the antibody webpage. To learn more about...
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CRISPR Plasmids - Drosophila
TypeCollection...Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 49410... -
CRISPR Plasmids - Yeast
TypeCollection...(s) Validated In Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 49014... -
Chemogenetics AAV Preps
TypeCollection...PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement Serotype PI 44361 pAAV-hSyn-DIO-hM3D... -
CRISPR Plasmids - Bacteria
TypeCollection...(s) Validated In Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 42875... -
Open Enzyme Collection
TypeCollection... Depositor Collections FreeGenes Project Open Enzyme Collection... -
Control AAV Preps
TypeCollection...quality AAV preps from select plasmids in the repository. Browse our control AAV collection, including... -
University of Florida Serotype Testing Panel for the Eye and Brain
TypeCollection...quality AAV preps from select plasmids in the repository. Browse the University of Florida serotype testing... -
Tetracycline Inducible Expression
TypeCollection...shRNA efficacy using fluorescence; use with pGFPns-reporter for cDNA target rtTA On Gu 60495 pSBtet-GP Sleeping... -
TALENs for Endogenous Zebrafish Genes
TypeCollection...TALENs for Endogenous Zebrafish Gene Targets Recent reports have shown that engineered TALENs can be used to... -
Optogenetics AAV Preps
TypeCollection...quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV collection, including... -
CRISPR Guide
TypeGuide...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...following the amplification protocol specified by the depositing lab. After amplification, you’ll need to use ... target sequence, it promiscuously cleaves the reporter as well, and a quantifiable signal is produced...CRISPR 101 eBook (3rd edition) CRISPR software and depositor protocols JOVE video reprint: Protocol for genomic...NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s from...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman... -
Deisseroth INTRSECT Collection
TypeCollection... Depositor Collections INTRSECT Plasmid Collection INTRSECT... -
The Pleiades Promoter Project
TypeCollection...MiniPromoters MiniPromoter Source Gene Construct Reporter Negative Control N/A pEMS1312 cre N/A pEMS1301... -
Genomic Deletions in Mammalian Cell Lines
TypeCollection...transfection conditions for each cell line with a reporter construct to ensure robust plasmid delivery before... -
Plan Your Experiment
TypeGuide...promoter is typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive...vector or separate transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify...Additional Resources: CRISPR protocols from Addgene depositors JOVE Video Reprint: Protocol for genomic deletions...in question (see CRISPR protocols from Addgene depositors ). Deliver Cas9 and gRNA Choose a delivery method...facilitates CRISPR/Cas9-based genome editing. Scientific Reports . 5 , 11315. PMID: 26066060 Zuris, J. A., Thompson... -
Allen Institute for Brain Science AAV Enhancer Collection
TypeCollection... Depositor Collections Allen Institute for Brain Science ... -
Optogenetics Guide
TypeGuide...optical switches Sensors are genetically-encoded reporters of molecular signals; e.g., calcium indicators...optogenetics tool - they typically allow the fast depolarization of neurons upon exposure to light through direct... trafficks well to the membrane. Excitatory (Depolarizing) ChR Variants. The optogenetic toolbox has been...light-gated, cation channels resulting in excitation (depolarization) of the neuron. Feature enhancements include... -
Guide to Using Pooled Libraries
TypeGuide... to amplify the library, please refer to the depositor’s protocol for the best results. For some libraries...die or do not pass selection (in the case of a reporter) Sort “winning” cells Sequence those cells that...Resources Science Exchange Scientist (formally Assay Depot) Resources Find additional resources on our website... -
Sequencing Primers
TypeGuide...that Addgene uses for sequence verification of deposited plasmids....distribute primers. For sequencing plasmids in our repository, we've chosen primers based on the plasmid backbone...of primers for sanger sequence verification of deposited plasmids. Below is a list of commonly used primers...