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...which may produce aerosols or splashes. These procedures should be done underneath a hood. Clean any spills...

...loading dye Electrophoresis buffer Pipet tips Procedure Select restriction enzymes to digest your plasmid...

...the best ratio of mass DNA:mass PEI determined. Procedure Seed HEK293T cells at 3.8x10 6 cells per plate...

...stock solution into 5 mL PBS. Protect from light. Procedure Section 1: Seeding cells Place a sterile poly-...

...transduction, and not during the cell seeding step. Procedure Before beginning a colony formation assay, the...

...fit on a microscope stage) Reagents None needed Procedure Set up your microscope by removing any protective...

...the P2 this represents the third decimal place. Procedure How to Pipette Now that you know the different...

...antibody for primary antibodies raised in a mouse. Procedure Section 1: Lyse cells Centrifuge 5 x 10 6 cells...

...Before Starting Warm reagents to room temperature. Procedure Section 1: Prepare the Antigen Standard and coat...

...with the volatile reagents in the list above. Procedure Option #1 - Solution D Protocol Before starting...