Skip to main content
Addgene
Showing: 621 - 640 of 861 results

  4. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...experiments.  In this blog post let's take a look at some of the other open resources discussed at the meeting... (Schwartz and Jorgensen, 2018).  These are just some examples of worm resources and tools openly shared...

  6. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ... of reasons for this but the primary one is that some behavioral experiments require stimulation only ... in this primer on optogenetics we’ll delve into some of the important considerations when performing ...

  7. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ... need to collect will depend on the serotype, as some rAAV serotypes are abundantly released into the ...10.1089/hum.2010.107 Zen, Z., Espinoza, Y., Bleu, T., Sommer, J. M., & Wright, J. F. (2004). Infectious titer...

  8. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...like BioLegend’s Fluorophore Guide Chart.  Pro tip! Some antibodies go by different names depending on your...specific value between the two channels. It is something your computer will do for you to better analyze...

  9. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...Planck Institute of Colloids and Interfaces. Here are some tips for cell culture that will hopefully help you...that cross-contamination can be identified. Even if some mammalian cells lines have similar morphologies,...

  10. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...thermostable periplasmic endonuclease that can sometimes co-purify with your plasmid DNA and lead to degradation...reached your lysate volume limits, the copy number of some origins of replication (e.g. p15A, ColE1) can be...

  11. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...many facts but the training of the mind to think something that cannot be learned from textbooks” (Brian,...Biochemistry and Molecular Biology Annual Meeting. Some of my students published their work identifying ...

  12. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ... We can all acknowledge that negative data is something every researcher experiences and that it often... J., Bahr-Davidson, J., Smith, P. H., Zhen, Z., Sommer, J. M., Pierce, G. F., & Qu, G. (2005). Identification...

  13. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...how to create and use a standard curve and provide some general considerations for scientists planning to...of the unknown sample. The unknown should fall somewhere in the middle of the standard curve. If you do...

  14. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ... to produce your own recombinant antibodies with some molecular biology and cell culture experience. Let...immunocytochemistry. It’s important to note that some antibodies are better suited for certain applications...

  16. Tips for arabidopsis transformation

    Type
    Blog Post
    ...sounds simple, and it pretty much is, but there are some tips and tricks you can use to obtain optimum transformation... this process right the first time. I’ll lay out some guidelines here. A detailed transformation protocol...

  17. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ... plasmid assembly (if primer design is correct). Some DNA sequences are not easy to amplify by PCR, so...Betaine (1 M) to the PCR mix. When PCR is not possible Some plasmids just won’t amplify! Examples of this are...

  18. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes prefer targets with a given single...Exchange) system (Abudayyeh et al., 2019). To avoid some of the inherent drawbacks for RNA editing of natural...

  20. DNA Purification Without a Kit

    Type
    Blog Post
    ...reach for that silica spin column, stop to consider some ways to purify DNA without a kit. DNA purification...precipitated, dried, and then rehydrated. There are some slight modifications depending on the type of DNA...
Showing: 621 - 640 of 861 results