We narrowed to 702 results for: abo.1

...testing. You can find Nick on LinkedIn. References 1. Skloot, Rebecca, and Bahni Turpin. The immortal life...Learn All about Mammalian Vectors Read up on CRISPR Delivery Systems for Mammalian Cells Learn about the Dangers...Misidentified or cross-contaminated cells have been known about since 1966 when Stanley Gartler reported that most...there is a loss of reputation that can impede collaborations, especially with industry. Today we also have...The LGC Group also provides standards and has laboratories to carry our independent quality control for...culture contamination." Archives of pathology & laboratory medicine 133.9 (2009): 1463-1467. PubMed PMID...

...declaration, only a small fraction (<1%) of authors have asked for advice about the scope of the policy. End ...the data policy was raised by researchers worried about the effort to make the underlying data easily available...making data available, but there remains concern about the extra work this may entail.  So if access to...be archived? Simplifying data access Some of the above mentioned issues could be mitigated by on-line services...him on Twitter @jwoodgett.     More Discussions About Data Sharing: PLOS Data Sharing Policy Discussion...Scholarly Kitchen) Read more Addgene blog posts about scientific sharing ...

...mammalian expression from human elongation factor 1 alpha Constitutive  Tends to give consistent expression...Promoter of the arabinose metabolic operon Inducible by arabinose and repressed catabolite repression in the ...Browse All Plasmids 101 Posts Learn about Mammalian Vectors Read about Reporter Gene like Luciferase and...and operators (response elements). Promoters are about 100 to 1000 base pairs long and found upstream of...this list is a great place to start, the tables above do not delve into the tissue or development-specific...

... reached labs in over 75 countries. Figure 1: SARS-CoV-2 proteins and other components. Image from...different SARS-CoV-2 spike variants. You can learn more about their research in this article. To see other plasmids... optimized. Browse the collection or learn more about it on the blog. COVID-19 research is happening fast...through plasmid sharing and more. As you’ve read above, some of these plasmids have been in the repository...Additional resources on the Addgene blog Learn more about mapping the SARS-CoV-2 - human protein-protein interaction... interaction Find tips for remote collaborations Browse all COVID-19 blog posts Resources on Addgene....

...such as model systems and primary cells. References 1. Doench, John G., et al. "Rational design of highly...Read this blog post about CRISPR Kinome Libraries available at Addgene Learn about the advantages of pooled...large-scale functional screens Read this blog post about genome-wide screening using CRISPR Resources on...libraries for the mouse genome! Other Resources Read about CRISPRi and CRISPRa from the NIH E-newsletter ...

...commandments for making it all work – most of the time. #1 Thou shalt find enjoyment in your work If you are ...on the Society for Laboratory Automation and Screening (SLAS) Electronic Laboratory Neighborhood.   Work...worry less about how they will handle an emergency and this will make you much more confident about their ...

...inclusion bodies and is folding properly. References 1. Angov, Evelina, et al. "Heterologous protein expression...that lead to these preferences and what can we do about them? Read on to find out!   Why do organisms have...understood, but some possible reasons include: Metabolic pressures - it takes cellular resources to produce...amino acid I’d like to encode,” but, as described above, not every region of a protein should necessarily...Effects on transcription (remember, it’s not all about translation - e.g. codon choice may interrupt transcription...

...off-target effects (Zibitt et al. 2021).    Figure 1: A schematic illustration of CRISPR/Cas9-based approaches... to devise an experiment that can tell you more about its function.  Resources and references Resources...

...(RBD) of the viral spike protein.   Figure 1. (A) SARS-CoV-2 RBD DMS Library generation and sequencing...acid, suggesting that MPyV system can improve metabolic pathway yields by encapsulating a single enzyme...

... digging deeply into their data.     Fig. 1: Genomic fraction for each of five major ancestry ...dependencies associated with each ancestry group. Since about 90% of the cell lines used in DepMap CRISPR screens...

...template including the desired edit. Figure 1: Prime Editing schematic. Created with BioRender.com... primer binding site, starting with a length of about 13 nt (Anzalone et al., 2019). Primer binding sites...of reverse transcriptase template, starting with about 10–16 nucleotides. For longer templates, it is even.... Test multiple nick sites, starting with sites about 50 bp upstream and downstream from the prime editing...

...determine purity of the AAV preparation. Figure 1: Silver staining of purified and non-purified AAV ... before being distributed to customers. Details about our production protocols, titering methods, and ...either in-house by Addgene scientists or through collaboration with viral vector manufacturing facilities, ...generated from lentiviral preparations (described above) that are subject to a concentration step prior ...

...drives our bioinformatics needs       Fig. 1: QC'ing data at Addgene is like standing under a Niagara...much information as possible from depositing laboratories and associated publications, but our quality...the process. Another design choice we appreciated about Nextflow was how effortless it was for us to switch...interface. In our next post, we’ll share our story about how we used Nextflow Tower to augment our pipeline...

...antibody bonding strengths.    . Steps of an IP: 1) Protein extracts from cells or tissues contain a ...proteins or nucleic acids. Read on to learn more about this versatile technique.  Immunoprecipitation ...proteins in an IP are not denatured (learn more about antibodies here!) When using Protein A/G to immobilize...eluted by boiling the beads in SDS. (To learn more about Western blotting check out our blog post The Basics...

...plasmids are passed down through generations (Figure 1). These so called “plasmid addiction systems” require... more about CcdB, the toxic key to efficient cloning Read all Plasmids 101 blog posts Read about knockout...with the other toxin-antitoxin systems described above, one part of the pair is more stable than the other...

...antibiotic selection. Essentially, this gene is a 2-in-1 providing both bacterial and eukaryotic selection,...therefore prevent cell death. One of the cool things about amp is that it's exclusively toxic to dividing cells...antibiotic selection. If you feel like multiple of the above antibiotics would work for you, they likely do! ...

...designed to shuttle between species. Figure 1: Shuttle vectors are vectors that can function in ...your experiment, meaning you don’t have to worry about vector replication at all. In these cases, transient...place in. In many cases, you won’t need to worry about the expression of your gene of interest in both ...

...sample’s absorption at 260 nm (A260). Figure 1: Characteristic absorption spectra for pure DNA and...flow-through solutions and use clean tubes? How about spin times, were these long enough?  There are, ...

... end sequences (R and L ends).  Figure 1: (A) Components involved in each type of CRISPR-Transposon..., 2020), to multi-copy genomic integration of metabolic cassettes (Zhang et al. 2020); however, the technology...

...your virus. Let’s start there!     Figure 1: Flowchart showing the process for using pooled lentiviral...ordering a pre-made library, you don’t need to worry about amplifying and producing lentivirus. But pre-made...