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We narrowed to 1,141 results for: Gene

Showing: 641 - 660 of 1141 results

  1. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...17179932. Additional Resources on the Addgene Blog AAVs CREATed for Gene Delivery to the CNS and PNS Advanced...Neuroscientist's View Control Gene Expression with FLEx Vectors Resources on Addgene.org AAV Vector for TVA Expression...selectively infect a genetically defined cell population. While the EnvA genetic sequences are supplied...combination with molecular tools (e.g., optogenetic or chemogenetic receptors) enables complex control of...enter cells and deliver a variety of cargoes, genetically engineered neurotropic viruses emerged as powerful...individual neurons they infect. However, they generally are not transmitted to adjacent neurons via synapses...of the fact that RABV is an enveloped virus. In general, viral envelopes dictate infection of target cells...

  2. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ... selectable with the antibiotic whose resistance gene is encoded in the viral vector. This antibiotic ...Martin L. Yarmush. "Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent...aren’t working, you can check if virus is being generated by your transfected packaging cells. This check...and transduction. Once you’ve harvested, it’s generally a good idea to titer your virus. Getting an accurate...standardize your experiments later. Stay tuned to the Addgene blog and be sure to check out our viral vector ... PMCID: PMC330758. Additional Resources on the Addgene Blog Learn All about Adenovirus Associated Virus...Lentiviral Vectors Additional Resources on the Addgene.org Check out Our Viral Vector Guide Pages Find ...

  3. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...Medicine. He is interested in everything related to gene expression, particularly at the RNA level. He maintains...fluorescent proteins and imaging technologies to study gene expression, nuclear localization, and dynamics” ...flux, etc). The most commonly used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others...More examples of these biosensors can be found at Addgene. Split FPs – some FPs (e.g. GFP, Venus) can be...proteins interact. Oligomerization: The first generations of FPs were prone to oligomerize. This may affect...More Helpful Websites & Resources: FP guide at Addgene Interactive Visualization of Fluorescent Protein...

  4. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    ...research (check out this article in the GSA’s blog Genes to Genomes about a TMPRSS2 plasmid from Roger Reeve...! Thank you depositors! In fact, this year, Addgene depositors quickly stepped up to make COVID-19 ... 2,300 COVID-19 related plasmids currently in Addgene’s repository which have been requested about 1,500...whether you are a depositor or not. We will have Addgene trivia (with prizes!), polls, and shout outs, and... and we’ll be featuring the work of the Addgene community. Perhaps you’ll find plasmids and new tools ...We’ll also take a look back on some fascinating Addgene history that you might not have known about. Here's...Thursday: Throwback Thursday (Blue Flame awards and Addgene history) Friday: Wrap up! To show the magnitude...

  5. Distribution to Industry

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    Collection
    ...currently available to industry scientists. ID Plasmid Gene/Insert PI Kits Kit name Type PI Description High...Packaging from Addgene Parkinson's Research from The Michael J Fox Foundation Search Use Addgene's Search to...protection for material providers. Addgene’s deposit agreements allow Addgene to distribute plasmids on behalf...can be submitted to Addgene through your user account or by email to mta@addgene.org . After we review ...A portion of Addgene’s collection is available to qualified for-profit entities. Addgene reserves the ...Addgene has a growing collection of plasmids that are available to industry. Learn more about the available...assembling standardized parts to create eukaryotic multigene constructs. Pooled Libraries Pooled Library name...

  6. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...Right: T1 tomato plants that inherited an edited gene to confer resistance to the herbicide chlorsulfuron...mouse monoclonal antibodies targeting the human genes and are recommended for use in immunohistochemistry...contributing to the Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [...Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...indicator ONE-GO biosensors kit Antibodies for neurodegeneration research   Optimized prime editing in dicots...applicable to dicot species. In addition, they generated offspring tomato plants that inherited the genomic...single-molecule fluorescence techniques. After generating a series of single- and double-tagged constructs...

  7. Degrading DNA with Cascade-Cas3

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    Blog Post
    ...therapeutics. Imagine deleting a problematic copy of a gene that causes a debilitating disease! This is, of ... and applications with the type I CRISPR system. Gene and Genome Editing, 3–4, 100013. https://doi.org...crime, Cascade. Find Cascade-Cas3 plasmids in Addgene's collection! A “complex” system Cas3 is part of...that the cell can use to repair. Figure 3: General overview of the Cascade-Cas3 mechanism. Created... CRISPR-Transposons Additional resources on Addgene.org CRISPR guide Cascade-Cas3 Plasmid collection...

  8. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ... your gene of interest plus two packaging plasmids (three packaging plasmids for 3rd generation lentivirus...produce adeno-associated virus type 2 vectors. Human Gene Therapy, 13(16), 1935–43. https://doi.org/10.1089...Your Lentivirus Preps More resources on Addgene.org Addgene's virus protocols Viral Plasmids and Resources...Insect cells are a less common option for the generation of viruses, though they are a popular choice ...much larger packaging capacity — up to 38 kb! Generally, insect cell production methods produce lower ...Resources and references More resources on the Addgene blog Viral Vectors 101: Producing Your rAAV AAV...

  9. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ... depending on whether the heavy and light chain genes are contained to one plasmid or if they’ve been ... L, Gervais C, Grazzini E, Durocher Y. Transient Gene Expression in Suspension HEK293-EBNA1 Cells. Methods...Antibodies Resources on addgene.org Addgene's Antibody Purification Protocol Addgene's Video Antibody Purification...of recombinant antibodies (rAbs) exist (though Addgene has a great collection of ready-to-use rAbs and... are contained in (Randolph et. al., 2018). At Addgene, we ensure the efficacy of our antibodies through...Resources and references More resources on the Addgene blog Introduction to Antibodies Plasmid-based Recombinant...Purification Protocol Addgene's Antibody Guide References Trimmer JS. Recombinant Antibodies in Basic Neuroscience...

  10. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...recombinant adeno-associated viral vectors." Human Gene Therapy ja (2019). PubMed PMID: 31140327. PubMed...vector genome titers by droplet digital PCR." Human gene therapy methods 25.2 (2013): 115-125. PubMed PMID...found that generating the standard curve for qPCR can be challenging to get right. Here at Addgene, we use...droplet quality. Using a droplet generator, the sample and the droplet generation oil are moved through small...Since Addgene began producing AAV vector preparations, we have used quantitative PCR to evaluate the ...virus before adding your sample to a mastermix. At Addgene, we typically load 3 dilutions onto our ddPCR plate...Pipette slowly If you are using a manual droplet generator, you will have to be careful about transferring...

  11. Tips for arabidopsis transformation

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    Blog Post
    ...transcriptional and translational dynamics of genes and proteins in living plants, to complementing mutant phenotypes...potato contains Agrobacterium T-DNAs with expressed genes: An example of a naturally transgenic food crop....simplifying plant genetic engineering Using arabidopsis in the classroom Resources on Addgene.org Find plant... transformation. There are many motivations to generate transgenic Arabidopsis, from studying transcriptional... floral drip or dip transformation method. The general steps for this method include: Cloning and transforming...Glazebrook, 2002). Find plant plasmid resources at Addgene  Choosing an expression system Choosing an appropriate...appropriate expression vector is the first step in generating transgenic Arabidopsis. There are a bunch of ...

  12. Deciphering the Mysteries of Behavior with Viral Vectors

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    Blog Post
    .... With the ability of viral vectors to fine tune gene expression temporally and spatially, exciting times... insights into the complex relationship between genes, neurons, and behavior. There are several other ...Scientists from the University of Geneva (Creed et al., 2015) used optogenetics to support these existing findings...are combining genetic manipulations with the forced swim test understanding the genetic background of ...Guide to Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...behavior. We’ve previously discussed some of the general considerations for performing behavioral tests,...within the LC of mice. When those neurons are chemogenetically activated, norepinephrine release causes a...

  13. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post
    ...plasmid (pEffector) expresses all seven required genes and the CRISPR RNA under the same promoter, and ...genomic insertions and/or deletions, to multiplexed gene knockouts (Vo et al., 2020), to multi-copy genomic...a wide variety of applications - as mutagenesis tools for genetic studies (e.g. using mariner), as a method...Medical Center. DNA transposons are ubiquitous genetic elements capable of spreading within and between... CRISPR-Cas, these systems represent the next generation of tools for efficient and programmable insertions... specifically recognized during transposition, genetic cargos must be flanked by the L and R ends - thus... at programmable insertions of even very large genetic cargo, at one or multiple desired genomic loci....

  14. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...negative bacteria periplasm. Efforts to tag endogenous genes for proteins in these compartments using CRISPR ...tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila...quantitative imaging experiments and cell physiology in general. Pitfalls of Fluorescent Protein Fusions Observing...cellular compartments other than the cytoplasm. Generally, FPs evolved in or were engineered for use in ...N-glycosylation consensus sequences in popular FPs. This generated a palette of inert green, cyan, yellow, and blue...standard FPs. The moxFPs are now available through Addgene Many thanks to our guest bloggers Erik L. Snapp...Central PMCID: PMC2150754. Resources Avaliable at Addgene Read Our Blog Post on Protein Tags Read Other ...

  15. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...promoter. Use this for doxycycline inducible (tet-off) gene expression! We've also added AAV1 and AAV5 preparations...variants of a new optogenetic tool called PdCO, deposited by the Yizhar lab. Addgene is now offering AAV1... Brian O'Neill.  More resources on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector...What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share...to our viral vectors repository since March.  Addgene is now offering an AAV2 CAG-driven tTA expresser...AAV5 preparations of several variants of a new optogenetic tool called PdCO.  This is an "optoGPCR" (coupled...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...

  16. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    ...Gladys will use plasmids containing a variety of HPV genes as positive controls in her PCR screens. She hopes... Sciences in Ghana will be receiving the third Addgene/Seeding Labs plasmid grant and plans to use the...future. Future awardees We will be opening the Addgene/Seeding Labs Plasmid Grant to a third round of ...inspiring research. Additional Resources on the Addgene Blog Learn How We Raised Money for Rare Disease...Grants to Rare Disease Research Resources on Addgene.org Check out our Cancer Pages Learn about Global...

  17. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ...Resources on the Addgene Blog Important Considerations When Using AAVs AAVs Are Great Tools for Gene Expression...Check out Addgene’s Step-By-Step Protocol Pressed For Time? Order Ready-to-Use AAV from Addgene ...Expression in Mammals Resources on Addgene.org Find AAV Packaging and Transfer Plasmids Here New to Making...

  18. Editor's Choice, July 2016

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    Blog Post
    ...the Cell Matter? AAV: A Versatile Viral Tool for Gene Expression in Mammals Recombinase-based State Machines...excellent posts from July: Addgene at the ASM Microbe 2016 Conference Generating Mouse Models Using CRISPR... them. July 2016 was a fantastic month for the Addgene blog. We achieved roughly 50,000 views over the...perturbations to how CRISPR has made it much easier to generate mouse models.   Cpf1 Update: Comparison to Cas9... Science Communications Intern (and soon to be Addgene Scientist!) Mary Gearing, this post focuses on ...

  19. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ...6 letter representation of the gene (or DNA sequence). pBACKBONE-hGene Tip: Often researchers will add...pBACKBONE-Flag-hGene(Q295A)-EGFP  Wildtype version: pBACKBONE-Flag-hGene-EGFP or pBACKBONE-Flag-hGene(wt)-EGFP...insert, you would list it first. pBACKBONE-Flag-hGene If there was also an EGFP fused to the C-terminal... would list it after the insert. pBACKBONE-Flag-hGene-EGFP Other considerations  If your insert contains...be included in the plasmid name. Mutations are generally listed as the amino acid change and not a nucleotide...

  20. Antibody Plasmid Collection

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    Collection
    ...-mouse" or "anti-rabbit") ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions ...Find information on Addgene's collection of plasmid-based antibodies, including monoclonal antibodies...Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant antibodies...application-specific validation and quality control by Addgene as well as by our trusted partner labs. Learn More...animals or hybridoma cells, but are difficult to generate due to expense and time. An alternative approach...fragment libraries from chickens Methods for the generation of chicken monoclonal antibody fragments by phage... 2015 Oct;167(2):95-102. Joanna Bereta Sybody Generation Toolbox for making synthetic nanobodies (sybodies...
Showing: 641 - 660 of 1141 results