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We narrowed to 1,148 results for: Gene

Showing: 641 - 660 of 1148 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... vector for gene expression pINDUCER21 (ORF-EG) - Inducible lentiviral gene expression ...destination vector for gene expression Lentiviral backbones made by Addgene Addgene has an extensive collection...host cell and are designed for a user to insert a gene of interest. They may contain tags or fusion proteins...These vectors allow you to test the function of your gene of interest in a controlled environment under various...we will assume that you want to express a gene; however, we have included a function section for if you...If you want to drive expression of your favorite gene, you will need a plasmid with a promoter that...mTagBFP2 tag pNIC-GST-TEV-GG - Cloning of target gene with N-terminal GST tag and TEV cleavage Transient...

  2. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Drosophila ID Plasmid Gene/Insert Promoter PI Publication Return to top Plant ID Plasmid Gene/Insert Promoter...elegans ID Plasmid Gene/Insert Promoter PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter...CRISPR plasmids for activating or upregulating gene expression in various model organisms. CRISPR...peptide can increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator...activator to promoter or regulatory regions of your gene of interest. If the plasmid that you choose does... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...

  3. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...learn what Addgene can do to help. More resources on the Addgene blog Twenty Years of Addgene Sharing: ...researchers around the globe. Addgene’s educational resources As Addgene built out our scientific support...other videos on Addgene’s YouTube channel.   Figure 4: Global viewership of the Addgene YouTube channel...about virus, I learned from Addgene,” or “I learned to clone from the Addgene website.” We delight in seeing...When Addgene opened in 2004, we focused on creating a repository that made it easy to share plasmids,...accessible, due to a wide range of logistical barriers. Addgene worked to identify, reduce, and remove these logistical...accessible weren’t always the same thing, shaped both Addgene’s founding and our growth into the company we are...

  4. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...knock down gene expression by interfering with transcription. Design your gRNA to target your gene of interest...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Epigenetics To make targeted epigenetic modifications... to epigenetic modifiers. Design your gRNA to target a specific promoter or enhancer for your gene of ...at a much lower efficiency than NHEJ. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...populations of mutants for directed evolution. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...edits via homology-directed repair (HDR). ID Plasmid Gene/Insert Promoter Selectable Marker Publication Prime... the desired edits on an RT template. ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication...

  5. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...Community Addgene: In 2012 your lab first deposited plasmids with Addgene. In 2014, you joined Addgene's Advisory...lab. In June 2014, Dr. Fujii joined Addgene's Advisory Board.  Addgene: Your lab has worked extensively ... enChIP through Addgene. Retroviral Expression System for enChIP using CRISPR Addgene: Your lab recently...the plasmids of these systems to Addgene in the near future. Addgene: Has your lab been working on any...first knew Addgene around 2010 and found the scheme very nice at first sight in that Addgene takes care...ourselves without Addgene. So, when I received an invitation to join the Advisory Board of Addgene, I accepted...Hodaka Fujii lab's Addgene page and click the "Subscribe to Alerts" link in the Addgene Alerts box. References...

  6. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...expected to grow with the ever-expanding genetic toolbox generated by the continuous effort of numerous scientists...). GFP is the most popular, most widely used genetically encoded fluorescent probe. Several factors contribute...probe, it is the first choice when selecting a genetically encoded fluorescent tag. There are, however, ...1998). Another mutation (A206K) is necessary to generate the strictly monomeric EGFP variant, mEGFP (Zacharias..., followed by an intramolecular reaction that generates the chromophore (Tsien, 1998). Importantly, this...Thorn, 2017). These fluorescent analogues are generally added to the bathing solution and require uptake...adding GFP may alter diffusion rates. Smaller genetically encoded probes are based on cofactor binding ...

  7. Tetracycline Inducible Expression

    Type
    Collection
    ...promoter upstream of your gene of interest. If your gene of interest should be generally active and only turned...Tet-On Systems for Doxycycline-inducible Gene Expression . Curr Gene Ther, 16 (3), 156–167. https://doi.org...Tet-On system for regulated gene expression through viral evolution . Gene Ther, 13 (19), 1382–1390. https...downstream gene via its activation domain than simply removing the repressor. 2nd and 3rd Generation Systems...Learn about tetracycline-regulated gene expression and find plasmids for your experiments using tet- ...repressible promoters are powerful tools to control gene expression and study biological targets. The tetracycline... for rapid, efficient, and reversible control of gene expression in eukaryotes. To avoid secondary effects...

  8. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...If you’ve ordered a pooled library from Addgene, you may have been a little surprised at how this powerful...deposited many types of pooled libraries here at Addgene, including cDNA, barcoding, screening, and gRNA...gRNA libraries. Most of the pooled libraries at Addgene are lentiviral pooled libraries, though other types...types exist.  When you buy a pooled library from Addgene, you can either order a DNA pooled library, which...single tube, or a pre-made viral prep library. Addgene’s pooled libraries are typically DNA preps, but ... A) Amplification, validation, and lentiviral generation of a pooled library. An aliquot of the pooled...experiments. Pooled library lentiviral preps are generally aliquoted to reduce the number of freeze-thaw ...

  9. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    ... institution’s TTO or legal office first. Generally, Addgene is also waiting to receive that legal agreement...materials from Addgene can qualify. We also reward improvement against one’s own record Mention Addgene Master...question? Refer them to Addgene’s Tech Transfer Team: techtransfer@addgene.org Congratulations to the...between a TTO and Addgene, whose terms apply to all future deposits of materials with Addgene Master Reception...between a TTO and Addgene, whose terms apply to all future materials requests from Addgene MTA: material ...commercialization Additional resources on Addgene.org Addgene Help Center: Tech Transfer Technology Transfer...arrival of the newest plasmid you requested from Addgene...the plasmid that will help you run that last ...

  10. Engaging with science and society at pgEd

    Type
    Blog Post
    ... Resources on Addgene.org Learn how Addgene engages with the community on our Addgene gives back page...Initiatives for the Personal Genetics Education Project (pgEd). Advances in genetic technologies and other ...broadens the conversation about genetics pgEd, based at the Department of Genetics of Harvard Medical School...existing inequalities and injustices. The Personal Genetics Education Project (pgEd), where I currently work...work, strives to broaden the conversations about genetics and facilitate dialogue between scientists and...the personal, social and ethical implications of genetics. In these conversations, pgEd strives to be inclusive...and empowered to make their own decisions about genetics, both as individual consumers of technologies ...

  11. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...Resources at Addgene.org Find pooled libraries at Addgene Find basic lab protocols at Addgene ...As a tech at Addgene, part of my job, in addition to preparing DNA and generating/replenishing kits, ...information from the workshop. Future workshops for Addgene Addgene strives to be a great resource for the research...Technician at Addgene who is interested in medicine and music.       Additional Resources on the Addgene Blog ... use what we learn to improve processes in the Addgene lab. In this post, I’ll tell you a little bit about...excited to go there because both LabCentral and Addgene aim to make scientific advancement easier. NEBNext...extract libraries from previously fragmented DNA.The general process for library preparation using this kit ...

  12. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...your desired edit are all you need to generate a knock-in.   General considerations for designing a repair.... A Mouse Geneticist's practical guide to CRISPR applications. Singh P, et al. Genetics 114.169771; ...accurate mechanism for DSB repair. The process is generally error-free provided the DNA template used for ... DNA to the target sequence to be modified. In general, the insertion sites of the modification should...bases can be difficult, thus dsDNA plasmids are generally preferred for large insertions such as fluorescent...efficiency with circular plasmid templates is generally low; to increase the frequency of edits, researchers...targeting region from the vector. Alternatively, PCR-generated or commercially synthesized linear dsDNA templates...

  13. Pooled Library Amplifications

    Type
    Blog Post
    ...Libraries Resources on Addgene.org Addgene's Guide to Plasmid Pooled Libraries  Addgene's Pooled Library Collection...chemical transformations are generally cheaper and easier. One library that Addgene distributes with a chemical...contact us at deposit@addgene.org. Happy amplifying! More resources on the Addgene blog Genome-Wide Screening...If you’re depositing a pooled library with Addgene, you may be surprised to learn that we ask for an ...recombination and loss of plasmid diversity, so Addgene strives to distribute aliquots of the original ...requesting scientist receives a pooled library from Addgene, they’ll need to amplify the sample for further...developed for each individual pooled library; generalized amplification protocols tend not to work as well...

  14. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Plant ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Xenopus ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  15. The Twelve Days of CRISPR

    Type
    Blog Post
    ... CRISPR, Addgene gave to me: a primer on prime editing.  On the second day of CRISPR, Addgene gave to ...On the fourth day of CRISPR, Addgene gave to me: ways to use optogenetics to control CRISPR editing.  ... day of CRISPR, Addgene gave to me: updates on base editing from the first generation of base editors ...the eighth generation (and dual base editors, too!).  On the seventh day of CRISPR, Addgene gave to me... of CRISPR, Addgene gave to me: anti-CRISPR proteins!  On the ninth day of CRISPR, Addgene gave to me:...🎶 On the 12 days of CRISPR, Addgene gave to me… 🎶 A few years ago we used the #12DaysOfCRIPSR hashtag...detect nucleic acids.  On the third day of CRISPR, Addgene gave to me: tips and tricks for designing gRNAs...

  16. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ...no weak links in the chain.  Addgene: What about the Streptococcus pyogenes protein domain made it ideal...cover it. Addgene: OK, thanks for your time, and thanks for depositing your plasmids with Addgene. Howarth...lab at the University of Oxford is dedicated to generating new tools to manipulate biology based on molecular...based on a protein isolated from Streptococcus pyogenes that locks itself together, to produce irreversible...another important step forward by dissecting that S. pyogenes protein into three parts. Their efforts yielded... from the “S” in Streptococcus and the “py” in pyogenes), capable of locking two peptide tags together... and its potential uses. Developing a new tool Addgene: Where did you get the idea to develop SpyLigase...

  17. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...reference, Addgene commonly prepares NGS samples using both pre- and post-amplification DNA and generates PCR...CRISPR Libraries Resources on Addgene.org Browse Pooled Libraries at Addgene ...In addition to single plasmids, Addgene also distributes pooled plasmid libraries containing hundreds...million plasmids. These libraries are some of Addgene’s most exciting and versatile offerings! We recently...plasmids are amplified and none are lost. Each Addgene library has its own depositor transformation and...components of the library are represented. Typically, Addgene uses electrocompetent Stbl4 cells when performing...transformation efficiency. For a typical library, Addgene usually does four to eight electroporation reactions...

  18. Plant Plasmids and Resources

    Type
    Collection
    ... a new window) - Tools for gene and protein expression and mapping of gene expression in plants. Figshare...from genome engineering and gene regulation to fluorescent proteins. Addgene’s collection includes plasmids...plasmids containing promoters that drive general and species-specific gene expression (see the column ‘promoter...vectors: A modular plasmid assembly kit for multigene expression, gene silencing, and silencing rescue in plants...protocol (Link opens in a new window) for Targeted Gene Mutation in Rice - Yinong Yang. The MoClo CRISPR...a high efficiency intron-optimized SpCas9-coding gene, zCas9i, and an intronized Cas9 series of vectors...The following collections can be used for studying gene expression in plants: Maize Transcription Factor...

  19. Synthetic Biology - Networks and Gene Regulation

    Type
    Collection
    ...Synthetic Biology Networks and Gene Regulation Synthetic Biology: Networks and Gene Regulation SynBio Resources...Addgene's collection of synthetic biology plasmids related to Networks and Gene Regulation Plasmid...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...regulatory elements, pre-assembled genetic circuits, and higher level gene networks. Examples Include: promoters...repressors and activators logic gates Networks and Gene Regulation Plasmids Search the table by keyword ...plasmids available from this depositor's lab. Plasmid Gene/Insert Vector Type Promoter PI Publication Back ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  20. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...', style='' %} Resources on the Addgene blog Subscribe to the Addgene blog Read our viral vector blog...blog posts Learn more about Addgene Resources on the Addgene website Find plasmids for your research  ...Addgene has so much to celebrate! As we enter this new growth phase of the organization and the expansion...and the opportunities we have for the future. At Addgene we will never ever stop planning, perfecting, and...new projects. Diversification is happening. The Addgene team will have some fun challenges this year and...get excited about making their first deposit to Addgene. We are also lauded by another set of stakeholders...we received some entirely unsolicited praise: “Addgene, for one, is a sterling example of collaboration...
Showing: 641 - 660 of 1148 results