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We narrowed to 1,146 results for: Gene

Showing: 641 - 660 of 1146 results

  1. AAV Molecular Tools

    Type
    Collection
    ...Long Overexpression Tools These AAV encode various genes for overexpression. ID Name Expression System Activity...Ready-to-use AAV available from Addgene's viral service encoding tet-off transactivators and tools for...

  2. Making CRISPR Plasmids Using Fragmid

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    Blog Post
    ...for stable fly cell lines; Hygromycin resistance gene; for genome integration by spontaneous insertion...own Fragmid components! Ensure your backbone and genes contain only the Bbsl cut sites needed for the Fragmid...plasmids on the Addgene website. Step 4: Naming your assembled plasmid and generating the vector file....2024.100519 More resources from Addgene Plasmids 101: Modular Cloning Addgene's CRISPR Guide Plasmids 101: ...been looking for just the right CRISPR vectors on Addgene and found instead ones that were… pretty close,...select the components they want for each module, generate the vector file, and quickly clone their vector...Fragmid components are available for request on the Addgene website, both as individual plasmids and as the...

  3. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...you attempt to verify the starting methionine of a gene but the blastx results start the alignment at a ...unlikely to have an exact entry for your favorite gene fused to an epitope tag or fusion protein. If your... our Snapgene-powered plasmid maps. Resources on Addgene.org Sequence Analysis of your Addgene Plasmid...This post was updated on Dec 4, 2017. At Addgene, we continually use the Basic Local Alignment Search...process has changed with the introduction of next generation sequencing!  Choosing a BLAST program Of the...databases, and elements of the BLAST search pages. At Addgene, we use blastn to identify any discrepancies in... viewing blastx results. When using blastx at Addgene, we use the default “Non-redundant protein sequences...

  4. 8 Great Depositor Research Stories

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    Blog Post
    ...tool for large inserts.  DAP arrays for multiplex gene editing: Guest blogger Qichen Yuan tells us about... share on our blog, please reach out to news@addgene.org - we'd love to hear from you!       ...

  5. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    Blog Post
    ...years, but lay readers may be less familiar with gene drives and their ramifications. Readers unfamiliar...in Doug Prasher, who originally isolated the GFP gene but was not included amongst the Nobel prize winners...time on Addgene’s role in the rapid dissemination of these technologies, proposing that “Addgene, with ...contributed to the steadily increasing distrust the general public has in science and scientists (as discussed...replace scientists themselves.  The sections on optogenetics and CRISPR of course address some of the ethical...has been a key driver of the technology.” As an Addgene vet of 12 years, I’m very familiar with the role...I discussed The State of Science with the five Addgene colleagues who read it with me, the consistent ...

  6. 10 Basic tips for mammalian cell culture

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    Blog Post
    ...Sana on LinkedIn.   References 1. Yin, R., et al. "Gene expression profiling analysis of bisphenol A-induced...induces cytoskeletal disorganization, apoptosis, and gene expression disregulation on HeLa cells." Journal...Also, avoid unnecessary talking because talking generates microbe-laden aerosols that may enter the hood...many times and could be contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage...Second  Edition), 2012. Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian...Plasmids 101: Mammalian Vectors Resources on Addgene.org Find mammalian plasmids for your research Find...

  7. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...17179932. Additional Resources on the Addgene Blog AAVs CREATed for Gene Delivery to the CNS and PNS Advanced...Neuroscientist's View Control Gene Expression with FLEx Vectors Resources on Addgene.org AAV Vector for TVA Expression...selectively infect a genetically defined cell population. While the EnvA genetic sequences are supplied...combination with molecular tools (e.g., optogenetic or chemogenetic receptors) enables complex control of...enter cells and deliver a variety of cargoes, genetically engineered neurotropic viruses emerged as powerful...individual neurons they infect. However, they generally are not transmitted to adjacent neurons via synapses...of the fact that RABV is an enveloped virus. In general, viral envelopes dictate infection of target cells...

  8. 5 Tips for Troubleshooting Viral Transductions

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    Blog Post
    ... selectable with the antibiotic whose resistance gene is encoded in the viral vector. This antibiotic ...Martin L. Yarmush. "Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent...aren’t working, you can check if virus is being generated by your transfected packaging cells. This check...and transduction. Once you’ve harvested, it’s generally a good idea to titer your virus. Getting an accurate...standardize your experiments later. Stay tuned to the Addgene blog and be sure to check out our viral vector ... PMCID: PMC330758. Additional Resources on the Addgene Blog Learn All about Adenovirus Associated Virus...Lentiviral Vectors Additional Resources on the Addgene.org Check out Our Viral Vector Guide Pages Find ...

  9. Which Fluorescent Protein Should I Use?

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    Blog Post
    ...Medicine. He is interested in everything related to gene expression, particularly at the RNA level. He maintains...fluorescent proteins and imaging technologies to study gene expression, nuclear localization, and dynamics” ...flux, etc). The most commonly used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others...More examples of these biosensors can be found at Addgene. Split FPs – some FPs (e.g. GFP, Venus) can be...proteins interact. Oligomerization: The first generations of FPs were prone to oligomerize. This may affect...More Helpful Websites & Resources: FP guide at Addgene Interactive Visualization of Fluorescent Protein...

  10. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    ...research (check out this article in the GSA’s blog Genes to Genomes about a TMPRSS2 plasmid from Roger Reeve...! Thank you depositors! In fact, this year, Addgene depositors quickly stepped up to make COVID-19 ... 2,300 COVID-19 related plasmids currently in Addgene’s repository which have been requested about 1,500...whether you are a depositor or not. We will have Addgene trivia (with prizes!), polls, and shout outs, and... and we’ll be featuring the work of the Addgene community. Perhaps you’ll find plasmids and new tools ...We’ll also take a look back on some fascinating Addgene history that you might not have known about. Here's...Thursday: Throwback Thursday (Blue Flame awards and Addgene history) Friday: Wrap up! To show the magnitude...

  11. Antibody Plasmid Collection

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    Collection
    ...-mouse" or "anti-rabbit") ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions ...Find information on Addgene's collection of plasmid-based antibodies, including monoclonal antibodies...Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant antibodies...application-specific validation and quality control by Addgene as well as by our trusted partner labs. Learn More...animals or hybridoma cells, but are difficult to generate due to expense and time. An alternative approach...fragment libraries from chickens Methods for the generation of chicken monoclonal antibody fragments by phage... 2015 Oct;167(2):95-102. Joanna Bereta Sybody Generation Toolbox for making synthetic nanobodies (sybodies...

  12. Distribution to Industry

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    Collection
    ...currently available to industry scientists. ID Plasmid Gene/Insert PI Kits Kit name Type PI Description High...Packaging from Addgene Parkinson's Research from The Michael J Fox Foundation Search Use Addgene's Search to...protection for material providers. Addgene's deposit agreements allow Addgene to distribute plasmids on behalf...can be submitted to Addgene through your user account or by email to mta@addgene.org . After we review ...A portion of Addgene's collection is available to qualified for-profit entities. Addgene reserves the ...Addgene has a growing collection of plasmids that are available to industry. Learn more about the available...assembling standardized parts to create eukaryotic multigene constructs. ONE-GO Biosensors Kit Fluorescent ...

  13. Deisseroth INTRSECT Collection

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    Collection
    ...opsins , genetically-encoded calcium indicators, and rabies targeting genes. Recombinases Addgene ID Plasmid...definitions (e.g. neurons defined by a single gene or projection). Intersectional expression approaches ... in well-defined cellular sub-populations has generally been limited to single-component cellular definitions...cells based on a doubly-specified combination of genetic and/or anatomical-defined parameters, by placing...Operating Procedure (Link opens in a new window) with general principles for working with INTRSECT recombinases...pAAV-CaMKIIa-Flpo None No Single recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral...Vcre No Dual recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral...

  14. The Pleiades Promoter Project

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    Collection
    ... disorders by enabling region- and cell-specific gene-delivery in the mouse brain through these human ...List of Pleiades MiniPromoters MiniPromoter Source Gene Construct Reporter Ple2 ADORA2A pEMS1142 EGFP/NLS...

  15. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post
    ...plasmid (pEffector) expresses all seven required genes and the CRISPR RNA under the same promoter, and ...genomic insertions and/or deletions, to multiplexed gene knockouts (Vo et al., 2020), to multi-copy genomic...a wide variety of applications - as mutagenesis tools for genetic studies (e.g. using mariner), as a method...Medical Center. DNA transposons are ubiquitous genetic elements capable of spreading within and between... CRISPR-Cas, these systems represent the next generation of tools for efficient and programmable insertions... specifically recognized during transposition, genetic cargos must be flanked by the L and R ends - thus... at programmable insertions of even very large genetic cargo, at one or multiple desired genomic loci....

  16. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    .... With the ability of viral vectors to fine tune gene expression temporally and spatially, exciting times... insights into the complex relationship between genes, neurons, and behavior. There are several other ...Scientists from the University of Geneva (Creed et al., 2015) used optogenetics to support these existing findings...are combining genetic manipulations with the forced swim test understanding the genetic background of ...Guide to Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...behavior. We’ve previously discussed some of the general considerations for performing behavioral tests,...within the LC of mice. When those neurons are chemogenetically activated, norepinephrine release causes a...

  17. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...Right: T1 tomato plants that inherited an edited gene to confer resistance to the herbicide chlorsulfuron...mouse monoclonal antibodies targeting the human genes and are recommended for use in immunohistochemistry...contributing to the Addgene Antibody Data Hub.  Figure 5: Immunohistochemistry with Addgene's Anti-APP [...Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...indicator ONE-GO biosensors kit Antibodies for neurodegeneration research   Optimized prime editing in dicots...applicable to dicot species. In addition, they generated offspring tomato plants that inherited the genomic...single-molecule fluorescence techniques. After generating a series of single- and double-tagged constructs...

  18. Degrading DNA with Cascade-Cas3

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    Blog Post
    ...therapeutics. Imagine deleting a problematic copy of a gene that causes a debilitating disease! This is, of ... and applications with the type I CRISPR system. Gene and Genome Editing, 3–4, 100013. https://doi.org...crime, Cascade. Find Cascade-Cas3 plasmids in Addgene's collection! A “complex” system Cas3 is part of...that the cell can use to repair. Figure 3: General overview of the Cascade-Cas3 mechanism. Created... CRISPR-Transposons Additional resources on Addgene.org CRISPR guide Cascade-Cas3 Plasmid collection...

  19. Production of Virus in Insect Versus Mammalian Cells

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    Blog Post
    ... your gene of interest plus two packaging plasmids (three packaging plasmids for 3rd generation lentivirus...produce adeno-associated virus type 2 vectors. Human Gene Therapy, 13(16), 1935–43. https://doi.org/10.1089...Your Lentivirus Preps More resources on Addgene.org Addgene's virus protocols Viral Plasmids and Resources...Insect cells are a less common option for the generation of viruses, though they are a popular choice ...much larger packaging capacity — up to 38 kb! Generally, insect cell production methods produce lower ...Resources and references More resources on the Addgene blog Viral Vectors 101: Producing Your rAAV AAV...

  20. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ... depending on whether the heavy and light chain genes are contained to one plasmid or if they’ve been ... L, Gervais C, Grazzini E, Durocher Y. Transient Gene Expression in Suspension HEK293-EBNA1 Cells. Methods...Antibodies Resources on addgene.org Addgene's Antibody Purification Protocol Addgene's Video Antibody Purification...of recombinant antibodies (rAbs) exist (though Addgene has a great collection of ready-to-use rAbs and... are contained in (Randolph et. al., 2018). At Addgene, we ensure the efficacy of our antibodies through...Resources and references More resources on the Addgene blog Introduction to Antibodies Plasmid-based Recombinant...Purification Protocol Addgene's Antibody Guide References Trimmer JS. Recombinant Antibodies in Basic Neuroscience...
Showing: 641 - 660 of 1146 results