We narrowed to 727 results for: ACE

... coat Close-toed shoes Gloves Eye protection and face shields, as needed Guidelines Your lab coat should...remain in the laboratory. When traveling between lab spaces, take your glove off of one hand and use your bare... on the type of sleeve. Lastly, don’t touch your face! Close-toed shoes and long pants should be worn ...your feet. Eye protection such as goggles and/or face shields can be used as needed. For BSL-2 work always...

...selection curve below. Carbenicillin is used here in place of ampicillin because carbenicillin is more stable... on top of the agar and gently spread over the surface until the liquid is mostly absorbed (there is a...visible volume of pooled liquid remaining on the surface). We use the micropipette tip itself to do the ...suspension onto the agar and gently spread over the surface until the liquid is mostly absorbed. The spreading...

...two methods: Method #1 Place the mixed oligos in a 1.5mL microfuge tube. Place tube in 90-95°C hot block...temperature (~45 minutes). Method #2 Place mixed oligos in a PCR tube. Place tube in a thermocycler programmed...

...an automated ELISA plate washer. Seal the plate. Place the plate on a microplate shaker set at 400 rpm ...an automated ELISA plate washer. Seal the plate Place the plate on a microplate shaker set at 400 rpm ...

... opens in a new window) (3,180 cm 2 - the same surface area as 21 x T-175 flasks). Cell stacks provide...these cells are very loosely attached to the dish surface and should be handled carefully. Avoid touching...other than PES. AAV particles stick to many other surfaces, but do not stick to PES. Using a PES filter will... toward the cap. If the media touches the cap, replace it with a new one before putting the CS5 in the...

...oligos are cloned into the AgeI and EcoRI sites in place of the stuffer. The AgeI site is destroyed in most...one 7kb and one 1.9kb. Cut out the 7kb band and place in a sterile microcentrifuge tube. TIP: When visualizing... the media to remove the transfection reagent. Replace with 5 mL fresh DMEM + 10% FBS + penicillin/streptomycin...20 hours. Remove the virus-containing media and replace with fresh media. Do not add puromycin until at...should be worn at all times. Use plastic pipettes in place of glass pipettes or needles. Liquid waste should...

...at a lower voltage. You will want to have enough space around each band to cut without having DNA in other...Notes: To protect the UV box, it is a good idea to place the gel on a glass plate if available. Unlike the...than a certain total volume of gel per reaction. Place the gel in a labeled microfuge tube. Using a scale...

... from light. Procedure Section 1: Seeding cells Place a sterile poly-D-lysine coated coverslip in each...Alternative fixation methods such as methanol or acetone may be better for some applications. Remove the...mounting medium to the microscope slide. Gently place the coverslip on the microscope slide with the cell...

...dissolved in dH 2 O. *Carbenicillin can be used in place of ampicillin. Carbenicillin is more stable, so ...later if your forget your bottle in the autoclave. Place the gel mix in the autoclave and run on a setting...to allow them to dry. After overnight drying, we place the plates in a plastic bag with an absorbent material...

...Protocol Option #2) Water-saturated Phenol 2 M Sodium Acetate pH 4 Chloroform/Isoamyl alcohol (49:1) 75% Ethanol...hazards for these reagents. Work in a well-ventilated space and under a fume hood when working with the volatile...sequentially to 1 mL of lysate: Add 0.1 mL of 2 M sodium acetate (pH 4.0), mix thoroughly by inversion. Add 1 mL...

...until dried before overnight incubation if needed. Place 100 mL sterile LB at 4 ℃. Day 2 (morning) Before...transformation efficiency! Ensure that no arcing is taking place during electroporation. Arcing would manifest as...obtain sufficient intact pooled library even in the face of a recombined fraction but care must be taken ...

...cells AAV Purification by Iodixanol Gradient Ultracentrifugation Protocol Mulitchannel Pipetting Technique...personal protective equipment, setting up a clean workspace, and maintaining sterility while working. AAV ...

...Day 1: Transduce cells Day 2 (am): Remove media, replace with fresh media Day 4–5: Count fluorescent cells...Incubate for 48–72 h. Gently aspirate media and replace with 1 mL of PBS. Calculate the fraction of fluorescent-positive...

...2 (am): 18 h post transfection - Remove media, replace with fresh media Day 3 or more (am): Observe fluorescence...following morning, carefully aspirate the media. Replace the media with 15 mL of DMEM complete. Incubate...

... 2 (am): 18 h post-transfection. Remove media, replace with fresh media. Day 3-4 (am): Harvest virus Equipment...following morning, carefully aspirate the media. Replace the media with 10 mL of DMEM Complete or OptiPro...

...the lowest power objective to find your sample. Place your slide (or other sample type) on the microscope...stage. If using a slide, you can secure it into place using the metal clips on the stage. Turn on the ...

... is an excellent resource for choosing primers. Place thin-walled PCR tubes on ice Set up a 50 μL reaction...Reverse Primers DO NOT get added to a master mix. Place reaction tubes in PCR machine. Set annealing temperature...

...and transduce Cells Day 2–3 (am): Remove media, replace with fresh media containing selection reagent Day...important to monitor these cells regularly and replace the cell media. Cell death by some cells in the...

...versatile and allows for nearly any piece of DNA to be placed into a backbone vector of choice with minimal limitations... important to have nice crisp bands and to have space to cut out the bands. Because of this we recommend...

...remove the white sticker on the bottom of the gel. Place the gel in the electrophoresis chamber and secure...where dye is visible. Section 2: Staining the Gel Place the gel in a plastic tray with 100 mL of deionized...